Experiment: Rifampicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1256 and Pf1N1B4_1257 are separated by 117 nucleotides Pf1N1B4_1257 and Pf1N1B4_1258 are separated by 274 nucleotides Pf1N1B4_1258 and Pf1N1B4_1259 are separated by 8 nucleotides
Pf1N1B4_1256 - Transcriptional regulator, LysR family, at 1,437,176 to 1,438,105
_1256
Pf1N1B4_1257 - Bifunctional protein: zinc-containing alcohol dehydrogenase; quinone oxidoreductase ( NADPH:quinone reductase) (EC 1.1.1.-); Similar to arginate lyase, at 1,438,223 to 1,439,236
_1257
Pf1N1B4_1258 - FIG00956405: hypothetical protein, at 1,439,511 to 1,439,681
_1258
Pf1N1B4_1259 - Multidrug resistance transporter, Bcr/CflA family, at 1,439,690 to 1,440,886
_1259
Position (kb)
1438
1439
1440 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1437.369 kb on - strand, within Pf1N1B4_1256 at 1437.385 kb on + strand, within Pf1N1B4_1256 at 1437.385 kb on + strand, within Pf1N1B4_1256 at 1437.386 kb on - strand, within Pf1N1B4_1256 at 1437.530 kb on + strand, within Pf1N1B4_1256 at 1437.611 kb on + strand, within Pf1N1B4_1256 at 1437.673 kb on - strand, within Pf1N1B4_1256 at 1437.903 kb on + strand, within Pf1N1B4_1256 at 1438.024 kb on + strand at 1438.024 kb on + strand at 1438.024 kb on + strand at 1438.024 kb on + strand at 1438.025 kb on - strand at 1438.161 kb on + strand at 1438.162 kb on - strand at 1438.162 kb on - strand at 1438.222 kb on + strand at 1438.223 kb on - strand at 1438.223 kb on - strand at 1438.238 kb on + strand at 1438.238 kb on + strand at 1438.238 kb on + strand at 1438.239 kb on - strand at 1438.239 kb on - strand at 1438.239 kb on - strand at 1438.241 kb on + strand at 1438.242 kb on - strand at 1438.242 kb on - strand at 1438.487 kb on + strand, within Pf1N1B4_1257 at 1438.558 kb on + strand at 1438.558 kb on + strand, within Pf1N1B4_1257 at 1439.033 kb on + strand, within Pf1N1B4_1257 at 1439.033 kb on + strand, within Pf1N1B4_1257 at 1439.034 kb on - strand, within Pf1N1B4_1257 at 1439.034 kb on - strand, within Pf1N1B4_1257 at 1439.034 kb on - strand, within Pf1N1B4_1257 at 1439.034 kb on - strand, within Pf1N1B4_1257 at 1439.034 kb on - strand, within Pf1N1B4_1257 at 1439.034 kb on - strand, within Pf1N1B4_1257 at 1439.034 kb on - strand at 1439.249 kb on + strand at 1439.290 kb on - strand at 1439.342 kb on + strand at 1439.342 kb on + strand at 1439.342 kb on + strand at 1439.343 kb on - strand at 1439.343 kb on - strand at 1439.366 kb on + strand at 1439.366 kb on + strand at 1439.366 kb on + strand at 1439.366 kb on + strand at 1439.366 kb on + strand at 1439.366 kb on + strand at 1439.366 kb on + strand at 1439.367 kb on - strand at 1439.367 kb on - strand at 1439.367 kb on - strand at 1439.426 kb on + strand at 1439.426 kb on + strand at 1439.427 kb on - strand at 1439.437 kb on - strand at 1439.465 kb on + strand at 1439.465 kb on + strand at 1439.465 kb on + strand at 1439.466 kb on - strand at 1439.466 kb on - strand at 1439.466 kb on - strand at 1439.466 kb on - strand at 1439.479 kb on + strand at 1439.479 kb on + strand at 1439.493 kb on + strand at 1439.494 kb on - strand at 1439.494 kb on - strand at 1439.524 kb on + strand at 1439.613 kb on - strand, within Pf1N1B4_1258 at 1439.613 kb on - strand, within Pf1N1B4_1258 at 1439.813 kb on + strand, within Pf1N1B4_1259 at 1439.814 kb on - strand at 1439.814 kb on - strand, within Pf1N1B4_1259 at 1439.936 kb on + strand, within Pf1N1B4_1259 at 1439.937 kb on - strand, within Pf1N1B4_1259 at 1439.937 kb on - strand, within Pf1N1B4_1259 at 1440.042 kb on + strand, within Pf1N1B4_1259 at 1440.042 kb on + strand at 1440.042 kb on + strand, within Pf1N1B4_1259 at 1440.043 kb on - strand, within Pf1N1B4_1259 at 1440.043 kb on - strand, within Pf1N1B4_1259 at 1440.043 kb on - strand at 1440.177 kb on + strand, within Pf1N1B4_1259 at 1440.178 kb on - strand, within Pf1N1B4_1259 at 1440.178 kb on - strand, within Pf1N1B4_1259 at 1440.213 kb on + strand, within Pf1N1B4_1259 at 1440.213 kb on + strand, within Pf1N1B4_1259 at 1440.214 kb on - strand, within Pf1N1B4_1259
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml remove 1,437,369 - Pf1N1B4_1256 0.21 -1.0 1,437,385 + Pf1N1B4_1256 0.22 -0.5 1,437,385 + Pf1N1B4_1256 0.22 -0.1 1,437,386 - Pf1N1B4_1256 0.23 +0.1 1,437,530 + Pf1N1B4_1256 0.38 +0.7 1,437,611 + Pf1N1B4_1256 0.47 -0.2 1,437,673 - Pf1N1B4_1256 0.53 -1.2 1,437,903 + Pf1N1B4_1256 0.78 +0.1 1,438,024 + -0.5 1,438,024 + -0.3 1,438,024 + +1.0 1,438,024 + +0.1 1,438,025 - +0.6 1,438,161 + -0.5 1,438,162 - +1.3 1,438,162 - -0.6 1,438,222 + +2.1 1,438,223 - -1.0 1,438,223 - -0.5 1,438,238 + -1.0 1,438,238 + +0.3 1,438,238 + +1.1 1,438,239 - +0.0 1,438,239 - -0.3 1,438,239 - -0.6 1,438,241 + +1.5 1,438,242 - +0.1 1,438,242 - +0.8 1,438,487 + Pf1N1B4_1257 0.26 -2.0 1,438,558 + +1.1 1,438,558 + Pf1N1B4_1257 0.33 -1.2 1,439,033 + Pf1N1B4_1257 0.80 -0.1 1,439,033 + Pf1N1B4_1257 0.80 -0.3 1,439,034 - Pf1N1B4_1257 0.80 -1.2 1,439,034 - Pf1N1B4_1257 0.80 -1.7 1,439,034 - Pf1N1B4_1257 0.80 -2.2 1,439,034 - Pf1N1B4_1257 0.80 -1.3 1,439,034 - Pf1N1B4_1257 0.80 +0.0 1,439,034 - Pf1N1B4_1257 0.80 -0.4 1,439,034 - +0.8 1,439,249 + +1.9 1,439,290 - -0.3 1,439,342 + -0.9 1,439,342 + +1.9 1,439,342 + -2.5 1,439,343 - -2.0 1,439,343 - -0.0 1,439,366 + +1.1 1,439,366 + +0.4 1,439,366 + +0.5 1,439,366 + +1.1 1,439,366 + +1.7 1,439,366 + +0.1 1,439,366 + -1.9 1,439,367 - -0.5 1,439,367 - +0.5 1,439,367 - +0.2 1,439,426 + -0.6 1,439,426 + -0.3 1,439,427 - +0.0 1,439,437 - -0.9 1,439,465 + +0.5 1,439,465 + +1.4 1,439,465 + +0.8 1,439,466 - +0.1 1,439,466 - -0.2 1,439,466 - +1.1 1,439,466 - -0.9 1,439,479 + -0.7 1,439,479 + -0.4 1,439,493 + +0.7 1,439,494 - -0.2 1,439,494 - +1.7 1,439,524 + +1.1 1,439,613 - Pf1N1B4_1258 0.60 +0.4 1,439,613 - Pf1N1B4_1258 0.60 -0.1 1,439,813 + Pf1N1B4_1259 0.10 +1.5 1,439,814 - +0.1 1,439,814 - Pf1N1B4_1259 0.10 +0.5 1,439,936 + Pf1N1B4_1259 0.21 -1.2 1,439,937 - Pf1N1B4_1259 0.21 -0.5 1,439,937 - Pf1N1B4_1259 0.21 -1.2 1,440,042 + Pf1N1B4_1259 0.29 -2.0 1,440,042 + -1.2 1,440,042 + Pf1N1B4_1259 0.29 -0.5 1,440,043 - Pf1N1B4_1259 0.29 +0.1 1,440,043 - Pf1N1B4_1259 0.29 -0.3 1,440,043 - +0.1 1,440,177 + Pf1N1B4_1259 0.41 +1.1 1,440,178 - Pf1N1B4_1259 0.41 -0.9 1,440,178 - Pf1N1B4_1259 0.41 +0.7 1,440,213 + Pf1N1B4_1259 0.44 -2.0 1,440,213 + Pf1N1B4_1259 0.44 +1.2 1,440,214 - Pf1N1B4_1259 0.44 +1.1
Or see this region's nucleotide sequence