Experiment: KB-half, Pseudo11 supernatant, 100 uM FeCl3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_24945 and AO356_24950 overlap by 4 nucleotides AO356_24950 and AO356_24955 are separated by 191 nucleotides AO356_24955 and AO356_24960 overlap by 4 nucleotides
AO356_24945: AO356_24945 - pyridine nucleotide-disulfide oxidoreductase, at 5,651,701 to 5,652,957
_24945
AO356_24950: AO356_24950 - (2Fe-2S)-binding protein, at 5,652,954 to 5,653,190
_24950
AO356_24955: AO356_24955 - D-amino acid oxidase, at 5,653,382 to 5,654,497
_24955
AO356_24960: AO356_24960 - hydroxyproline-2-epimerase, at 5,654,494 to 5,655,426
_24960
Position (kb) 5653
5654
5655 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5652.454 kb on + strand, within AO356_24945 at 5652.663 kb on - strand, within AO356_24945 at 5652.751 kb on + strand, within AO356_24945 at 5652.752 kb on - strand, within AO356_24945 at 5652.752 kb on - strand, within AO356_24945 at 5652.752 kb on - strand, within AO356_24945 at 5652.752 kb on - strand, within AO356_24945 at 5653.180 kb on - strand at 5653.190 kb on + strand at 5653.191 kb on - strand at 5653.191 kb on - strand at 5653.210 kb on + strand at 5653.211 kb on - strand at 5653.211 kb on - strand at 5653.423 kb on - strand at 5653.423 kb on - strand at 5653.641 kb on + strand, within AO356_24955 at 5653.641 kb on + strand, within AO356_24955 at 5653.641 kb on + strand, within AO356_24955 at 5653.641 kb on + strand, within AO356_24955 at 5653.642 kb on - strand, within AO356_24955 at 5653.804 kb on - strand, within AO356_24955 at 5654.109 kb on - strand, within AO356_24955 at 5654.561 kb on + strand at 5654.736 kb on - strand, within AO356_24960 at 5654.736 kb on - strand, within AO356_24960 at 5654.802 kb on + strand, within AO356_24960 at 5654.860 kb on + strand, within AO356_24960 at 5654.867 kb on + strand, within AO356_24960 at 5654.867 kb on + strand, within AO356_24960 at 5654.867 kb on + strand, within AO356_24960 at 5654.868 kb on - strand, within AO356_24960 at 5655.017 kb on + strand, within AO356_24960
Per-strain Table
Position Strand Gene LocusTag Fraction KB-half, Pseudo11 supernatant, 100 uM FeCl3 remove 5,652,454 + AO356_24945 0.60 -2.2 5,652,663 - AO356_24945 0.77 -0.2 5,652,751 + AO356_24945 0.84 -0.2 5,652,752 - AO356_24945 0.84 -0.2 5,652,752 - AO356_24945 0.84 -0.2 5,652,752 - AO356_24945 0.84 +0.4 5,652,752 - AO356_24945 0.84 -2.2 5,653,180 - -1.2 5,653,190 + +0.4 5,653,191 - -1.2 5,653,191 - -1.2 5,653,210 + +0.6 5,653,211 - -0.2 5,653,211 - +0.8 5,653,423 - -1.2 5,653,423 - +0.4 5,653,641 + AO356_24955 0.23 -1.7 5,653,641 + AO356_24955 0.23 +1.4 5,653,641 + AO356_24955 0.23 -2.5 5,653,641 + AO356_24955 0.23 -0.2 5,653,642 - AO356_24955 0.23 -3.2 5,653,804 - AO356_24955 0.38 -2.2 5,654,109 - AO356_24955 0.65 +0.4 5,654,561 + -1.2 5,654,736 - AO356_24960 0.26 -0.2 5,654,736 - AO356_24960 0.26 +0.8 5,654,802 + AO356_24960 0.33 +0.8 5,654,860 + AO356_24960 0.39 +0.1 5,654,867 + AO356_24960 0.40 -0.2 5,654,867 + AO356_24960 0.40 -1.2 5,654,867 + AO356_24960 0.40 -0.2 5,654,868 - AO356_24960 0.40 -2.2 5,655,017 + AO356_24960 0.56 +1.2
Or see this region's nucleotide sequence