Experiment: KB-half, Pseudo11 supernatant, 100 uM FeCl3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_24815 and AO356_24820 are separated by 340 nucleotides AO356_24820 and AO356_24825 are separated by 129 nucleotides AO356_24825 and AO356_24830 overlap by 8 nucleotides
AO356_24815: AO356_24815 - diguanylate cyclase, at 5,623,852 to 5,626,425
_24815
AO356_24820: AO356_24820 - calcium-binding protein, at 5,626,766 to 5,627,152
_24820
AO356_24825: AO356_24825 - hypothetical protein, at 5,627,282 to 5,628,127
_24825
AO356_24830: AO356_24830 - hypothetical protein, at 5,628,120 to 5,628,881
_24830
Position (kb)
5626
5627
5628 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5625.996 kb on + strand, within AO356_24815 at 5626.094 kb on + strand, within AO356_24815 at 5626.095 kb on - strand, within AO356_24815 at 5626.124 kb on + strand, within AO356_24815 at 5626.124 kb on + strand, within AO356_24815 at 5626.426 kb on - strand at 5626.431 kb on + strand at 5626.431 kb on + strand at 5626.432 kb on - strand at 5626.432 kb on - strand at 5626.432 kb on - strand at 5626.493 kb on + strand at 5626.597 kb on + strand at 5626.598 kb on - strand at 5626.601 kb on + strand at 5626.602 kb on - strand at 5626.602 kb on - strand at 5626.602 kb on - strand at 5626.602 kb on - strand at 5626.687 kb on + strand at 5626.688 kb on - strand at 5626.691 kb on + strand at 5626.692 kb on - strand at 5626.738 kb on + strand at 5626.739 kb on - strand at 5626.761 kb on - strand at 5626.761 kb on - strand at 5626.877 kb on + strand, within AO356_24820 at 5627.078 kb on + strand, within AO356_24820 at 5627.079 kb on - strand, within AO356_24820 at 5627.079 kb on - strand, within AO356_24820 at 5627.579 kb on + strand, within AO356_24825 at 5627.580 kb on - strand, within AO356_24825 at 5627.603 kb on + strand, within AO356_24825 at 5627.603 kb on + strand, within AO356_24825 at 5627.603 kb on + strand, within AO356_24825 at 5627.715 kb on - strand, within AO356_24825 at 5627.906 kb on + strand, within AO356_24825
Per-strain Table
Position Strand Gene LocusTag Fraction KB-half, Pseudo11 supernatant, 100 uM FeCl3 remove 5,625,996 + AO356_24815 0.83 -0.1 5,626,094 + AO356_24815 0.87 -1.7 5,626,095 - AO356_24815 0.87 +1.4 5,626,124 + AO356_24815 0.88 +0.9 5,626,124 + AO356_24815 0.88 +0.2 5,626,426 - -1.1 5,626,431 + +0.4 5,626,431 + -0.1 5,626,432 - -0.6 5,626,432 - -1.8 5,626,432 - -1.1 5,626,493 + -0.1 5,626,597 + +0.9 5,626,598 - +0.2 5,626,601 + +1.4 5,626,602 - +0.4 5,626,602 - -1.1 5,626,602 - -0.7 5,626,602 - +0.4 5,626,687 + +0.7 5,626,688 - -0.1 5,626,691 + -0.1 5,626,692 - -1.1 5,626,738 + -1.1 5,626,739 - +0.9 5,626,761 - +0.4 5,626,761 - -0.3 5,626,877 + AO356_24820 0.29 +0.1 5,627,078 + AO356_24820 0.81 -1.1 5,627,079 - AO356_24820 0.81 -1.1 5,627,079 - AO356_24820 0.81 +2.0 5,627,579 + AO356_24825 0.35 +1.4 5,627,580 - AO356_24825 0.35 +1.2 5,627,603 + AO356_24825 0.38 -0.6 5,627,603 + AO356_24825 0.38 -1.1 5,627,603 + AO356_24825 0.38 +1.9 5,627,715 - AO356_24825 0.51 -0.1 5,627,906 + AO356_24825 0.74 +2.2
Or see this region's nucleotide sequence