Experiment: KB-half, Pseudo11 supernatant, 100 uM FeCl3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_23510 and AO356_23515 are separated by 24 nucleotides AO356_23515 and AO356_23520 are separated by 61 nucleotides AO356_23520 and AO356_23525 are separated by 2 nucleotides
AO356_23510: AO356_23510 - amino acid ABC transporter permease, at 5,272,069 to 5,272,758
_23510
AO356_23515: AO356_23515 - ABC transporter substrate-binding protein, at 5,272,783 to 5,273,553
_23515
AO356_23520: AO356_23520 - fructoselysine kinase, at 5,273,615 to 5,274,469
_23520
AO356_23525: AO356_23525 - phosphosugar isomerase, at 5,274,472 to 5,275,485
_23525
Position (kb)
5273
5274
5275 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5272.812 kb on - strand at 5272.812 kb on - strand at 5272.815 kb on + strand at 5272.816 kb on - strand at 5272.816 kb on - strand at 5272.832 kb on + strand at 5272.832 kb on + strand at 5272.832 kb on + strand at 5272.832 kb on + strand at 5272.899 kb on + strand, within AO356_23515 at 5272.899 kb on + strand, within AO356_23515 at 5272.900 kb on - strand, within AO356_23515 at 5273.126 kb on + strand, within AO356_23515 at 5273.126 kb on + strand, within AO356_23515 at 5273.127 kb on - strand, within AO356_23515 at 5273.237 kb on + strand, within AO356_23515 at 5273.237 kb on + strand, within AO356_23515 at 5273.237 kb on + strand, within AO356_23515 at 5273.237 kb on + strand, within AO356_23515 at 5273.238 kb on - strand, within AO356_23515 at 5273.544 kb on - strand at 5273.544 kb on - strand at 5273.580 kb on - strand at 5274.103 kb on - strand, within AO356_23520 at 5274.138 kb on + strand, within AO356_23520 at 5274.138 kb on + strand, within AO356_23520 at 5274.138 kb on + strand, within AO356_23520 at 5274.139 kb on - strand, within AO356_23520 at 5274.139 kb on - strand, within AO356_23520 at 5274.307 kb on - strand, within AO356_23520 at 5274.343 kb on - strand, within AO356_23520 at 5274.375 kb on - strand, within AO356_23520 at 5274.406 kb on - strand at 5274.426 kb on + strand at 5274.427 kb on - strand at 5274.477 kb on - strand at 5274.494 kb on + strand at 5274.572 kb on + strand at 5274.572 kb on + strand at 5274.573 kb on - strand at 5274.573 kb on - strand at 5274.635 kb on + strand, within AO356_23525 at 5274.836 kb on + strand, within AO356_23525 at 5274.836 kb on + strand, within AO356_23525 at 5274.836 kb on + strand, within AO356_23525 at 5274.836 kb on + strand, within AO356_23525 at 5274.837 kb on - strand, within AO356_23525 at 5274.837 kb on - strand, within AO356_23525 at 5274.837 kb on - strand, within AO356_23525 at 5274.872 kb on + strand, within AO356_23525 at 5274.872 kb on + strand, within AO356_23525 at 5274.873 kb on - strand, within AO356_23525 at 5274.873 kb on - strand, within AO356_23525 at 5274.873 kb on - strand, within AO356_23525 at 5274.873 kb on - strand, within AO356_23525 at 5274.893 kb on + strand, within AO356_23525 at 5274.927 kb on + strand, within AO356_23525 at 5274.928 kb on - strand, within AO356_23525 at 5275.061 kb on + strand, within AO356_23525 at 5275.061 kb on + strand, within AO356_23525 at 5275.061 kb on + strand, within AO356_23525 at 5275.062 kb on - strand, within AO356_23525 at 5275.074 kb on + strand, within AO356_23525 at 5275.142 kb on - strand, within AO356_23525 at 5275.308 kb on - strand, within AO356_23525 at 5275.323 kb on - strand, within AO356_23525 at 5275.370 kb on + strand, within AO356_23525 at 5275.371 kb on - strand, within AO356_23525 at 5275.371 kb on - strand, within AO356_23525 at 5275.371 kb on - strand, within AO356_23525 at 5275.371 kb on - strand, within AO356_23525
Per-strain Table
Position Strand Gene LocusTag Fraction KB-half, Pseudo11 supernatant, 100 uM FeCl3 remove 5,272,812 - +1.4 5,272,812 - -0.2 5,272,815 + +1.0 5,272,816 - +0.6 5,272,816 - +1.0 5,272,832 + -0.0 5,272,832 + +1.8 5,272,832 + -0.7 5,272,832 + +0.6 5,272,899 + AO356_23515 0.15 -1.6 5,272,899 + AO356_23515 0.15 -0.4 5,272,900 - AO356_23515 0.15 +3.1 5,273,126 + AO356_23515 0.44 -2.0 5,273,126 + AO356_23515 0.44 -0.0 5,273,127 - AO356_23515 0.45 -1.0 5,273,237 + AO356_23515 0.59 -0.4 5,273,237 + AO356_23515 0.59 -2.0 5,273,237 + AO356_23515 0.59 -0.4 5,273,237 + AO356_23515 0.59 +1.0 5,273,238 - AO356_23515 0.59 -0.0 5,273,544 - -2.2 5,273,544 - -0.6 5,273,580 - -0.0 5,274,103 - AO356_23520 0.57 -2.6 5,274,138 + AO356_23520 0.61 -0.0 5,274,138 + AO356_23520 0.61 -1.0 5,274,138 + AO356_23520 0.61 -0.2 5,274,139 - AO356_23520 0.61 -1.0 5,274,139 - AO356_23520 0.61 -1.0 5,274,307 - AO356_23520 0.81 +2.0 5,274,343 - AO356_23520 0.85 -2.0 5,274,375 - AO356_23520 0.89 -1.0 5,274,406 - -1.0 5,274,426 + +1.6 5,274,427 - +1.0 5,274,477 - -1.0 5,274,494 + -0.0 5,274,572 + -0.3 5,274,572 + -0.5 5,274,573 - +0.3 5,274,573 - -2.6 5,274,635 + AO356_23525 0.16 -0.0 5,274,836 + AO356_23525 0.36 -1.8 5,274,836 + AO356_23525 0.36 +2.3 5,274,836 + AO356_23525 0.36 -0.7 5,274,836 + AO356_23525 0.36 +0.2 5,274,837 - AO356_23525 0.36 -0.0 5,274,837 - AO356_23525 0.36 -0.0 5,274,837 - AO356_23525 0.36 -2.0 5,274,872 + AO356_23525 0.39 -1.6 5,274,872 + AO356_23525 0.39 -1.0 5,274,873 - AO356_23525 0.40 -1.0 5,274,873 - AO356_23525 0.40 +1.1 5,274,873 - AO356_23525 0.40 +1.3 5,274,873 - AO356_23525 0.40 -0.0 5,274,893 + AO356_23525 0.42 -1.0 5,274,927 + AO356_23525 0.45 +1.4 5,274,928 - AO356_23525 0.45 -1.0 5,275,061 + AO356_23525 0.58 -0.8 5,275,061 + AO356_23525 0.58 -2.0 5,275,061 + AO356_23525 0.58 +0.6 5,275,062 - AO356_23525 0.58 +1.0 5,275,074 + AO356_23525 0.59 -0.0 5,275,142 - AO356_23525 0.66 -2.0 5,275,308 - AO356_23525 0.82 -1.0 5,275,323 - AO356_23525 0.84 -1.6 5,275,370 + AO356_23525 0.89 -0.4 5,275,371 - AO356_23525 0.89 +0.6 5,275,371 - AO356_23525 0.89 -1.0 5,275,371 - AO356_23525 0.89 -1.0 5,275,371 - AO356_23525 0.89 -1.0
Or see this region's nucleotide sequence