Experiment: KB-half, Pseudo11 supernatant, 100 uM FeCl3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_23455 and AO356_23460 are separated by 154 nucleotides AO356_23460 and AO356_23465 are separated by 56 nucleotides AO356_23465 and AO356_23470 are separated by 58 nucleotides
AO356_23455: AO356_23455 - diguanylate cyclase, at 5,257,486 to 5,259,132
_23455
AO356_23460: AO356_23460 - porin, at 5,259,287 to 5,259,985
_23460
AO356_23465: AO356_23465 - polyketide cyclase, at 5,260,042 to 5,260,512
_23465
AO356_23470: AO356_23470 - polyketide synthase, at 5,260,571 to 5,263,981
_23470
Position (kb) 5259
5260 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5258.375 kb on + strand, within AO356_23455 at 5258.376 kb on - strand, within AO356_23455 at 5258.376 kb on - strand, within AO356_23455 at 5258.382 kb on + strand, within AO356_23455 at 5258.834 kb on + strand, within AO356_23455 at 5258.834 kb on + strand, within AO356_23455 at 5258.834 kb on + strand, within AO356_23455 at 5258.835 kb on - strand, within AO356_23455 at 5258.854 kb on - strand, within AO356_23455 at 5258.982 kb on - strand at 5258.982 kb on - strand at 5258.987 kb on + strand at 5258.987 kb on + strand at 5258.987 kb on + strand at 5258.988 kb on - strand at 5258.988 kb on - strand at 5258.988 kb on - strand at 5259.134 kb on + strand at 5259.134 kb on + strand at 5259.134 kb on + strand at 5259.134 kb on + strand at 5259.134 kb on + strand at 5259.134 kb on + strand at 5259.134 kb on + strand at 5259.134 kb on + strand at 5259.134 kb on + strand at 5259.135 kb on - strand at 5259.135 kb on - strand at 5259.135 kb on - strand at 5259.135 kb on - strand at 5259.135 kb on - strand at 5259.135 kb on - strand at 5259.135 kb on - strand at 5259.135 kb on - strand at 5259.135 kb on - strand at 5259.135 kb on - strand at 5259.135 kb on - strand at 5259.135 kb on - strand at 5259.176 kb on - strand at 5259.289 kb on - strand at 5259.331 kb on - strand at 5259.405 kb on + strand, within AO356_23460 at 5259.406 kb on - strand, within AO356_23460 at 5259.442 kb on - strand, within AO356_23460 at 5259.442 kb on - strand, within AO356_23460 at 5259.543 kb on + strand, within AO356_23460 at 5259.565 kb on - strand, within AO356_23460 at 5259.565 kb on - strand, within AO356_23460 at 5259.565 kb on - strand, within AO356_23460 at 5259.609 kb on + strand, within AO356_23460 at 5259.609 kb on + strand, within AO356_23460 at 5259.678 kb on + strand, within AO356_23460 at 5259.888 kb on + strand, within AO356_23460 at 5259.889 kb on - strand, within AO356_23460 at 5259.977 kb on + strand at 5259.989 kb on - strand at 5260.014 kb on + strand at 5260.015 kb on - strand at 5260.045 kb on + strand at 5260.046 kb on - strand at 5260.175 kb on + strand, within AO356_23465 at 5260.656 kb on + strand at 5260.656 kb on + strand at 5260.656 kb on + strand at 5260.657 kb on - strand at 5260.657 kb on - strand at 5260.657 kb on - strand at 5260.657 kb on - strand at 5260.657 kb on - strand at 5260.824 kb on + strand at 5260.824 kb on + strand at 5260.824 kb on + strand at 5260.825 kb on - strand at 5260.825 kb on - strand at 5260.825 kb on - strand at 5260.864 kb on - strand at 5260.864 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB-half, Pseudo11 supernatant, 100 uM FeCl3 remove 5,258,375 + AO356_23455 0.54 -0.2 5,258,376 - AO356_23455 0.54 +0.4 5,258,376 - AO356_23455 0.54 +0.1 5,258,382 + AO356_23455 0.54 +1.2 5,258,834 + AO356_23455 0.82 +0.3 5,258,834 + AO356_23455 0.82 +0.3 5,258,834 + AO356_23455 0.82 +2.2 5,258,835 - AO356_23455 0.82 +0.3 5,258,854 - AO356_23455 0.83 -0.9 5,258,982 - -0.2 5,258,982 - +0.8 5,258,987 + +0.8 5,258,987 + -0.3 5,258,987 + +2.0 5,258,988 - -0.8 5,258,988 - +0.8 5,258,988 - -1.0 5,259,134 + -1.0 5,259,134 + +0.9 5,259,134 + -0.3 5,259,134 + -2.0 5,259,134 + +1.6 5,259,134 + +1.6 5,259,134 + -1.0 5,259,134 + -3.0 5,259,134 + -0.7 5,259,135 - +0.9 5,259,135 - +1.2 5,259,135 - -2.3 5,259,135 - -0.6 5,259,135 - +0.3 5,259,135 - -1.4 5,259,135 - -0.0 5,259,135 - -0.4 5,259,135 - +1.3 5,259,135 - -0.6 5,259,135 - -1.0 5,259,135 - -0.1 5,259,176 - -0.0 5,259,289 - +1.0 5,259,331 - +0.2 5,259,405 + AO356_23460 0.17 +0.3 5,259,406 - AO356_23460 0.17 -0.3 5,259,442 - AO356_23460 0.22 -0.4 5,259,442 - AO356_23460 0.22 +2.3 5,259,543 + AO356_23460 0.37 -1.0 5,259,565 - AO356_23460 0.40 +1.1 5,259,565 - AO356_23460 0.40 +0.8 5,259,565 - AO356_23460 0.40 -0.2 5,259,609 + AO356_23460 0.46 +1.6 5,259,609 + AO356_23460 0.46 -1.0 5,259,678 + AO356_23460 0.56 +1.0 5,259,888 + AO356_23460 0.86 -0.0 5,259,889 - AO356_23460 0.86 +1.6 5,259,977 + -0.7 5,259,989 - +2.2 5,260,014 + +1.0 5,260,015 - +0.3 5,260,045 + +0.8 5,260,046 - +1.0 5,260,175 + AO356_23465 0.28 -0.0 5,260,656 + -0.3 5,260,656 + +2.2 5,260,656 + -0.0 5,260,657 - -0.2 5,260,657 - +1.2 5,260,657 - -0.8 5,260,657 - -1.0 5,260,657 - +1.7 5,260,824 + -0.0 5,260,824 + +0.2 5,260,824 + -0.4 5,260,825 - +1.0 5,260,825 - -0.4 5,260,825 - +1.0 5,260,864 - -0.4 5,260,864 - -0.6
Or see this region's nucleotide sequence