Experiment: KB-half, Pseudo11 supernatant, 100 uM FeCl3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_05980 and AO356_05985 overlap by 4 nucleotides AO356_05985 and AO356_05990 are separated by 105 nucleotides AO356_05990 and AO356_05995 are separated by 3 nucleotides AO356_05995 and AO356_06000 are separated by 45 nucleotides
AO356_05980: AO356_05980 - UDP-N-acetylglucosamine acyltransferase, at 1,326,304 to 1,327,080
_05980
AO356_05985: AO356_05985 - 3-hydroxyacyl-ACP dehydratase, at 1,327,077 to 1,327,517
_05985
AO356_05990: AO356_05990 - UDP-3-O-(3-hydroxymyristoyl) glucosamine N-acyltransferase, at 1,327,623 to 1,328,678
_05990
AO356_05995: AO356_05995 - hypothetical protein, at 1,328,682 to 1,329,185
_05995
AO356_06000: AO356_06000 - outer membrane protein assembly factor BamA, at 1,329,231 to 1,331,606
_06000
Position (kb) 1327
1328
1329 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1327.611 kb on - strand at 1327.622 kb on - strand at 1328.684 kb on - strand at 1328.729 kb on - strand at 1329.059 kb on - strand, within AO356_05995 at 1329.059 kb on - strand, within AO356_05995 at 1329.059 kb on - strand, within AO356_05995 at 1329.098 kb on - strand, within AO356_05995 at 1329.180 kb on - strand at 1329.180 kb on - strand at 1329.180 kb on - strand at 1329.180 kb on - strand at 1329.233 kb on - strand at 1329.233 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB-half, Pseudo11 supernatant, 100 uM FeCl3 remove 1,327,611 - +0.5 1,327,622 - +0.1 1,328,684 - -1.5 1,328,729 - +0.5 1,329,059 - AO356_05995 0.75 -0.9 1,329,059 - AO356_05995 0.75 -2.5 1,329,059 - AO356_05995 0.75 +0.5 1,329,098 - AO356_05995 0.83 -2.1 1,329,180 - -2.8 1,329,180 - -2.5 1,329,180 - -2.1 1,329,180 - -1.5 1,329,233 - -0.8 1,329,233 - -1.5
Or see this region's nucleotide sequence