Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34245 and RR42_RS34250 are separated by 158 nucleotides RR42_RS34250 and RR42_RS34255 are separated by 218 nucleotides
RR42_RS34245: RR42_RS34245 - branched-chain alpha-ketoacid:ferredoxin oxidoreductase, fused (from data) , at 3,108,318 to 3,111,878
_RS34245
RR42_RS34250: RR42_RS34250 - LysR family transcriptional regulator, at 3,112,037 to 3,112,942
_RS34250
RR42_RS34255: RR42_RS34255 - aldehyde dehydrogenase, at 3,113,161 to 3,114,681
_RS34255
Position (kb)
3112
3113 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3111.069 kb on - strand, within RR42_RS34245 at 3111.070 kb on + strand, within RR42_RS34245 at 3111.070 kb on + strand, within RR42_RS34245 at 3111.085 kb on + strand, within RR42_RS34245 at 3111.305 kb on - strand, within RR42_RS34245 at 3111.314 kb on - strand, within RR42_RS34245 at 3111.334 kb on + strand, within RR42_RS34245 at 3111.512 kb on - strand, within RR42_RS34245 at 3111.814 kb on + strand at 3111.814 kb on + strand at 3111.814 kb on + strand at 3111.814 kb on + strand at 3111.815 kb on - strand at 3111.815 kb on - strand at 3111.815 kb on - strand at 3111.920 kb on + strand at 3111.920 kb on + strand at 3111.921 kb on - strand at 3111.921 kb on - strand at 3111.921 kb on - strand at 3111.921 kb on - strand at 3111.994 kb on + strand at 3111.994 kb on + strand at 3111.994 kb on + strand at 3111.995 kb on - strand at 3111.995 kb on - strand at 3111.995 kb on - strand at 3112.051 kb on + strand at 3112.051 kb on + strand at 3112.052 kb on - strand at 3112.144 kb on + strand, within RR42_RS34250 at 3112.144 kb on + strand, within RR42_RS34250 at 3112.145 kb on - strand, within RR42_RS34250 at 3112.343 kb on + strand, within RR42_RS34250 at 3112.343 kb on + strand, within RR42_RS34250 at 3112.343 kb on + strand, within RR42_RS34250 at 3112.344 kb on - strand, within RR42_RS34250 at 3112.344 kb on - strand, within RR42_RS34250 at 3112.344 kb on - strand, within RR42_RS34250 at 3112.344 kb on - strand, within RR42_RS34250 at 3112.358 kb on + strand, within RR42_RS34250 at 3112.359 kb on - strand, within RR42_RS34250 at 3112.499 kb on - strand, within RR42_RS34250 at 3112.554 kb on + strand, within RR42_RS34250 at 3112.625 kb on + strand, within RR42_RS34250 at 3112.625 kb on + strand, within RR42_RS34250 at 3112.625 kb on + strand, within RR42_RS34250 at 3112.626 kb on - strand, within RR42_RS34250 at 3112.626 kb on - strand, within RR42_RS34250 at 3112.626 kb on - strand, within RR42_RS34250 at 3112.626 kb on - strand, within RR42_RS34250 at 3112.626 kb on - strand, within RR42_RS34250 at 3112.626 kb on - strand, within RR42_RS34250 at 3112.670 kb on + strand, within RR42_RS34250 at 3112.768 kb on - strand, within RR42_RS34250 at 3112.785 kb on - strand, within RR42_RS34250 at 3112.941 kb on - strand at 3112.941 kb on - strand at 3113.050 kb on + strand at 3113.050 kb on + strand at 3113.051 kb on - strand at 3113.198 kb on - strand at 3113.215 kb on + strand at 3113.215 kb on + strand at 3113.215 kb on + strand at 3113.224 kb on + strand at 3113.264 kb on - strand at 3113.606 kb on - strand, within RR42_RS34255 at 3113.875 kb on - strand, within RR42_RS34255
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 3,111,069 - RR42_RS34245 0.77 -0.9 3,111,070 + RR42_RS34245 0.77 -3.9 3,111,070 + RR42_RS34245 0.77 -1.5 3,111,085 + RR42_RS34245 0.78 -0.8 3,111,305 - RR42_RS34245 0.84 -0.4 3,111,314 - RR42_RS34245 0.84 -3.9 3,111,334 + RR42_RS34245 0.85 -2.6 3,111,512 - RR42_RS34245 0.90 -2.7 3,111,814 + -1.6 3,111,814 + -1.0 3,111,814 + -2.2 3,111,814 + -0.0 3,111,815 - -3.5 3,111,815 - -4.4 3,111,815 - -1.4 3,111,920 + -2.4 3,111,920 + -5.3 3,111,921 - -0.4 3,111,921 - +0.3 3,111,921 - -1.3 3,111,921 - -3.3 3,111,994 + +1.0 3,111,994 + -1.0 3,111,994 + -2.3 3,111,995 - -0.1 3,111,995 - -0.4 3,111,995 - -2.7 3,112,051 + -4.4 3,112,051 + -2.0 3,112,052 - -3.0 3,112,144 + RR42_RS34250 0.12 -1.8 3,112,144 + RR42_RS34250 0.12 -3.0 3,112,145 - RR42_RS34250 0.12 -2.6 3,112,343 + RR42_RS34250 0.34 -0.4 3,112,343 + RR42_RS34250 0.34 -2.7 3,112,343 + RR42_RS34250 0.34 -3.4 3,112,344 - RR42_RS34250 0.34 -0.5 3,112,344 - RR42_RS34250 0.34 -4.0 3,112,344 - RR42_RS34250 0.34 -3.0 3,112,344 - RR42_RS34250 0.34 -2.9 3,112,358 + RR42_RS34250 0.35 -2.8 3,112,359 - RR42_RS34250 0.36 -1.8 3,112,499 - RR42_RS34250 0.51 -0.6 3,112,554 + RR42_RS34250 0.57 -3.3 3,112,625 + RR42_RS34250 0.65 -2.5 3,112,625 + RR42_RS34250 0.65 -3.3 3,112,625 + RR42_RS34250 0.65 -1.6 3,112,626 - RR42_RS34250 0.65 -2.7 3,112,626 - RR42_RS34250 0.65 -3.7 3,112,626 - RR42_RS34250 0.65 -1.6 3,112,626 - RR42_RS34250 0.65 -2.1 3,112,626 - RR42_RS34250 0.65 -1.2 3,112,626 - RR42_RS34250 0.65 -3.8 3,112,670 + RR42_RS34250 0.70 -1.4 3,112,768 - RR42_RS34250 0.81 -1.8 3,112,785 - RR42_RS34250 0.83 -2.8 3,112,941 - -1.1 3,112,941 - -0.6 3,113,050 + +1.4 3,113,050 + +0.0 3,113,051 - -0.2 3,113,198 - -1.1 3,113,215 + -1.0 3,113,215 + -0.3 3,113,215 + +0.2 3,113,224 + +1.0 3,113,264 - +0.5 3,113,606 - RR42_RS34255 0.29 +0.0 3,113,875 - RR42_RS34255 0.47 -1.0
Or see this region's nucleotide sequence