Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23545 and RR42_RS23550 are separated by 26 nucleotides RR42_RS23550 and RR42_RS23555 overlap by 8 nucleotides RR42_RS23555 and RR42_RS23560 overlap by 8 nucleotides RR42_RS23560 and RR42_RS23565 overlap by 14 nucleotides
RR42_RS23545: RR42_RS23545 - hypothetical protein, at 608,152 to 609,594
_RS23545
RR42_RS23550: RR42_RS23550 - flagellar export protein FliJ, at 609,621 to 610,076
_RS23550
RR42_RS23555: RR42_RS23555 - ATP synthase, at 610,069 to 611,532
_RS23555
RR42_RS23560: RR42_RS23560 - flagellar assembly protein FliH, at 611,525 to 612,382
_RS23560
RR42_RS23565: RR42_RS23565 - flagellar motor switch protein G, at 612,369 to 613,361
_RS23565
Position (kb)
610
611
612 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 609.075 kb on + strand, within RR42_RS23545 at 609.148 kb on - strand, within RR42_RS23545 at 609.334 kb on + strand, within RR42_RS23545 at 609.335 kb on - strand, within RR42_RS23545 at 609.585 kb on + strand at 609.667 kb on + strand, within RR42_RS23550 at 609.667 kb on + strand, within RR42_RS23550 at 609.667 kb on + strand, within RR42_RS23550 at 609.667 kb on + strand, within RR42_RS23550 at 609.668 kb on - strand, within RR42_RS23550 at 609.732 kb on - strand, within RR42_RS23550 at 609.732 kb on - strand, within RR42_RS23550 at 610.052 kb on + strand at 610.053 kb on - strand at 610.070 kb on + strand at 610.070 kb on + strand at 610.070 kb on + strand at 610.098 kb on + strand at 610.098 kb on + strand at 610.098 kb on + strand at 610.099 kb on - strand at 610.099 kb on - strand at 610.220 kb on + strand, within RR42_RS23555 at 610.221 kb on - strand, within RR42_RS23555 at 610.257 kb on - strand, within RR42_RS23555 at 610.266 kb on + strand, within RR42_RS23555 at 610.472 kb on + strand, within RR42_RS23555 at 610.472 kb on + strand, within RR42_RS23555 at 610.473 kb on - strand, within RR42_RS23555 at 610.673 kb on + strand, within RR42_RS23555 at 610.854 kb on + strand, within RR42_RS23555 at 611.362 kb on + strand, within RR42_RS23555 at 611.363 kb on - strand, within RR42_RS23555 at 611.523 kb on + strand at 611.524 kb on - strand at 611.524 kb on - strand at 611.524 kb on - strand at 611.524 kb on - strand at 611.789 kb on + strand, within RR42_RS23560 at 611.979 kb on + strand, within RR42_RS23560 at 611.979 kb on + strand, within RR42_RS23560 at 611.980 kb on - strand, within RR42_RS23560 at 612.028 kb on - strand, within RR42_RS23560 at 612.218 kb on - strand, within RR42_RS23560 at 612.376 kb on + strand at 612.376 kb on + strand at 612.376 kb on + strand at 612.377 kb on - strand at 612.377 kb on - strand at 612.399 kb on + strand at 612.430 kb on - strand at 612.430 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 609,075 + RR42_RS23545 0.64 +0.1 609,148 - RR42_RS23545 0.69 -0.3 609,334 + RR42_RS23545 0.82 +0.0 609,335 - RR42_RS23545 0.82 +0.7 609,585 + -1.2 609,667 + RR42_RS23550 0.10 +0.3 609,667 + RR42_RS23550 0.10 -0.5 609,667 + RR42_RS23550 0.10 +0.5 609,667 + RR42_RS23550 0.10 +0.5 609,668 - RR42_RS23550 0.10 -0.3 609,732 - RR42_RS23550 0.24 -0.9 609,732 - RR42_RS23550 0.24 +0.8 610,052 + -0.9 610,053 - -0.6 610,070 + +0.3 610,070 + +2.4 610,070 + +1.0 610,098 + -0.3 610,098 + +0.8 610,098 + +1.3 610,099 - -0.6 610,099 - +0.3 610,220 + RR42_RS23555 0.10 -1.4 610,221 - RR42_RS23555 0.10 -0.7 610,257 - RR42_RS23555 0.13 -0.5 610,266 + RR42_RS23555 0.13 -0.3 610,472 + RR42_RS23555 0.28 -0.6 610,472 + RR42_RS23555 0.28 +0.2 610,473 - RR42_RS23555 0.28 -0.8 610,673 + RR42_RS23555 0.41 -0.9 610,854 + RR42_RS23555 0.54 +0.1 611,362 + RR42_RS23555 0.88 +1.4 611,363 - RR42_RS23555 0.88 -0.1 611,523 + +1.0 611,524 - +0.1 611,524 - +0.1 611,524 - -0.0 611,524 - +3.1 611,789 + RR42_RS23560 0.31 -1.5 611,979 + RR42_RS23560 0.53 +0.2 611,979 + RR42_RS23560 0.53 -0.3 611,980 - RR42_RS23560 0.53 -0.7 612,028 - RR42_RS23560 0.59 +0.8 612,218 - RR42_RS23560 0.81 +1.0 612,376 + +0.2 612,376 + -0.3 612,376 + -0.7 612,377 - +1.3 612,377 - +0.3 612,399 + +1.8 612,430 - -2.0 612,430 - +0.6
Or see this region's nucleotide sequence