Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02305 and RR42_RS02310 are separated by 78 nucleotides RR42_RS02310 and RR42_RS02315 are separated by 15 nucleotides RR42_RS02315 and RR42_RS02320 are separated by 243 nucleotides
RR42_RS02305: RR42_RS02305 - porin, at 516,087 to 517,421
_RS02305
RR42_RS02310: RR42_RS02310 - NADPH-dependent 7-cyano-7-deazaguanine reductase, at 517,500 to 518,333
_RS02310
RR42_RS02315: RR42_RS02315 - threonine dehydratase, at 518,349 to 519,875
_RS02315
RR42_RS02320: RR42_RS02320 - trehalose phosphatase, at 520,119 to 520,904
_RS02320
Position (kb) 518
519
520 Strain fitness (log2 ratio) -2
-1
0
1
2 at 517.445 kb on + strand at 517.587 kb on - strand, within RR42_RS02310 at 517.700 kb on - strand, within RR42_RS02310 at 517.705 kb on - strand, within RR42_RS02310 at 517.870 kb on + strand, within RR42_RS02310 at 517.870 kb on + strand, within RR42_RS02310 at 517.871 kb on - strand, within RR42_RS02310 at 518.369 kb on - strand at 518.369 kb on - strand at 518.397 kb on + strand at 518.397 kb on + strand at 518.397 kb on + strand at 518.398 kb on - strand at 518.398 kb on - strand at 518.402 kb on - strand at 518.402 kb on - strand at 518.516 kb on + strand, within RR42_RS02315 at 518.587 kb on + strand, within RR42_RS02315 at 518.632 kb on + strand, within RR42_RS02315 at 518.632 kb on + strand, within RR42_RS02315 at 518.632 kb on + strand, within RR42_RS02315 at 518.757 kb on - strand, within RR42_RS02315 at 518.785 kb on + strand, within RR42_RS02315 at 518.995 kb on + strand, within RR42_RS02315 at 519.264 kb on + strand, within RR42_RS02315 at 519.265 kb on - strand, within RR42_RS02315 at 519.265 kb on - strand, within RR42_RS02315 at 519.296 kb on - strand, within RR42_RS02315 at 519.478 kb on + strand, within RR42_RS02315 at 519.479 kb on - strand, within RR42_RS02315 at 519.479 kb on - strand, within RR42_RS02315 at 519.479 kb on - strand, within RR42_RS02315 at 519.479 kb on - strand, within RR42_RS02315 at 519.491 kb on - strand, within RR42_RS02315 at 519.703 kb on + strand, within RR42_RS02315 at 519.797 kb on - strand at 519.797 kb on - strand at 519.826 kb on + strand at 519.826 kb on + strand at 519.856 kb on + strand at 519.856 kb on + strand at 519.857 kb on - strand at 519.918 kb on - strand at 519.918 kb on - strand at 520.066 kb on + strand at 520.112 kb on + strand at 520.113 kb on - strand at 520.130 kb on - strand at 520.497 kb on + strand, within RR42_RS02320 at 520.646 kb on + strand, within RR42_RS02320 at 520.697 kb on + strand, within RR42_RS02320 at 520.697 kb on + strand, within RR42_RS02320 at 520.698 kb on - strand, within RR42_RS02320
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 517,445 + +0.6 517,587 - RR42_RS02310 0.10 -1.3 517,700 - RR42_RS02310 0.24 -0.1 517,705 - RR42_RS02310 0.25 +1.2 517,870 + RR42_RS02310 0.44 +2.0 517,870 + RR42_RS02310 0.44 +0.8 517,871 - RR42_RS02310 0.44 -0.1 518,369 - +0.3 518,369 - -2.1 518,397 + +0.4 518,397 + +0.2 518,397 + +0.2 518,398 - +0.4 518,398 - -0.4 518,402 - -0.0 518,402 - +0.5 518,516 + RR42_RS02315 0.11 +1.4 518,587 + RR42_RS02315 0.16 -1.2 518,632 + RR42_RS02315 0.19 -0.3 518,632 + RR42_RS02315 0.19 -0.1 518,632 + RR42_RS02315 0.19 -0.8 518,757 - RR42_RS02315 0.27 -1.0 518,785 + RR42_RS02315 0.29 -0.6 518,995 + RR42_RS02315 0.42 +0.3 519,264 + RR42_RS02315 0.60 -2.1 519,265 - RR42_RS02315 0.60 +1.1 519,265 - RR42_RS02315 0.60 +0.7 519,296 - RR42_RS02315 0.62 -0.1 519,478 + RR42_RS02315 0.74 -0.6 519,479 - RR42_RS02315 0.74 -0.8 519,479 - RR42_RS02315 0.74 +1.0 519,479 - RR42_RS02315 0.74 -0.0 519,479 - RR42_RS02315 0.74 +0.1 519,491 - RR42_RS02315 0.75 -0.2 519,703 + RR42_RS02315 0.89 -1.7 519,797 - -0.5 519,797 - +0.6 519,826 + -0.8 519,826 + -2.5 519,856 + -0.1 519,856 + -0.2 519,857 - -0.1 519,918 - -0.1 519,918 - +0.1 520,066 + -0.4 520,112 + -0.4 520,113 - +0.8 520,130 - +1.2 520,497 + RR42_RS02320 0.48 -1.6 520,646 + RR42_RS02320 0.67 -1.8 520,697 + RR42_RS02320 0.74 -1.0 520,697 + RR42_RS02320 0.74 -1.3 520,698 - RR42_RS02320 0.74 +0.6
Or see this region's nucleotide sequence