Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01430 and RR42_RS01435 are separated by 96 nucleotides RR42_RS01435 and RR42_RS01440 are separated by 69 nucleotides RR42_RS01440 and RR42_RS01445 are separated by 155 nucleotides
RR42_RS01430: RR42_RS01430 - hypothetical protein, at 322,380 to 323,579
_RS01430
RR42_RS01435: RR42_RS01435 - short-chain dehydrogenase, at 323,676 to 324,422
_RS01435
RR42_RS01440: RR42_RS01440 - diguanylate cyclase, at 324,492 to 324,905
_RS01440
RR42_RS01445: RR42_RS01445 - tRNA 2-thiocytidine biosynthesis protein TtcA, at 325,061 to 326,002
_RS01445
Position (kb) 324
325 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 323.590 kb on + strand at 323.590 kb on + strand at 323.590 kb on + strand at 323.987 kb on + strand, within RR42_RS01435 at 323.993 kb on + strand, within RR42_RS01435 at 323.993 kb on + strand, within RR42_RS01435 at 324.321 kb on + strand, within RR42_RS01435 at 324.903 kb on + strand at 324.904 kb on - strand at 325.106 kb on + strand at 325.106 kb on + strand at 325.107 kb on - strand at 325.107 kb on - strand at 325.107 kb on - strand at 325.202 kb on + strand, within RR42_RS01445 at 325.202 kb on + strand, within RR42_RS01445 at 325.202 kb on + strand, within RR42_RS01445 at 325.203 kb on - strand, within RR42_RS01445 at 325.203 kb on - strand, within RR42_RS01445 at 325.203 kb on - strand, within RR42_RS01445 at 325.203 kb on - strand, within RR42_RS01445 at 325.203 kb on - strand, within RR42_RS01445 at 325.343 kb on + strand, within RR42_RS01445 at 325.343 kb on + strand, within RR42_RS01445 at 325.343 kb on + strand, within RR42_RS01445 at 325.343 kb on + strand, within RR42_RS01445 at 325.343 kb on + strand, within RR42_RS01445 at 325.344 kb on - strand, within RR42_RS01445 at 325.344 kb on - strand, within RR42_RS01445 at 325.344 kb on - strand, within RR42_RS01445 at 325.344 kb on - strand, within RR42_RS01445 at 325.344 kb on - strand, within RR42_RS01445 at 325.344 kb on - strand, within RR42_RS01445 at 325.344 kb on - strand, within RR42_RS01445 at 325.344 kb on - strand, within RR42_RS01445 at 325.368 kb on - strand, within RR42_RS01445 at 325.368 kb on - strand, within RR42_RS01445 at 325.445 kb on + strand, within RR42_RS01445 at 325.446 kb on - strand, within RR42_RS01445 at 325.446 kb on - strand, within RR42_RS01445 at 325.613 kb on + strand, within RR42_RS01445 at 325.641 kb on - strand, within RR42_RS01445 at 325.768 kb on + strand, within RR42_RS01445 at 325.768 kb on + strand, within RR42_RS01445 at 325.768 kb on + strand, within RR42_RS01445 at 325.768 kb on + strand, within RR42_RS01445 at 325.769 kb on - strand, within RR42_RS01445
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 323,590 + +1.2 323,590 + -0.2 323,590 + -0.1 323,987 + RR42_RS01435 0.42 -0.5 323,993 + RR42_RS01435 0.42 -0.3 323,993 + RR42_RS01435 0.42 +0.3 324,321 + RR42_RS01435 0.86 -3.3 324,903 + -0.2 324,904 - +0.6 325,106 + +0.0 325,106 + +0.1 325,107 - +0.3 325,107 - -0.2 325,107 - -0.0 325,202 + RR42_RS01445 0.15 -0.0 325,202 + RR42_RS01445 0.15 -0.0 325,202 + RR42_RS01445 0.15 -0.5 325,203 - RR42_RS01445 0.15 +1.0 325,203 - RR42_RS01445 0.15 -0.5 325,203 - RR42_RS01445 0.15 +0.2 325,203 - RR42_RS01445 0.15 +0.2 325,203 - RR42_RS01445 0.15 -0.2 325,343 + RR42_RS01445 0.30 -0.6 325,343 + RR42_RS01445 0.30 +1.1 325,343 + RR42_RS01445 0.30 -0.5 325,343 + RR42_RS01445 0.30 -0.2 325,343 + RR42_RS01445 0.30 +0.4 325,344 - RR42_RS01445 0.30 +0.6 325,344 - RR42_RS01445 0.30 +0.7 325,344 - RR42_RS01445 0.30 +0.7 325,344 - RR42_RS01445 0.30 -0.7 325,344 - RR42_RS01445 0.30 -0.2 325,344 - RR42_RS01445 0.30 -0.3 325,344 - RR42_RS01445 0.30 +0.8 325,344 - RR42_RS01445 0.30 +0.3 325,368 - RR42_RS01445 0.33 +0.9 325,368 - RR42_RS01445 0.33 -0.8 325,445 + RR42_RS01445 0.41 -0.3 325,446 - RR42_RS01445 0.41 +0.5 325,446 - RR42_RS01445 0.41 +0.7 325,613 + RR42_RS01445 0.59 -0.0 325,641 - RR42_RS01445 0.62 +0.1 325,768 + RR42_RS01445 0.75 -0.4 325,768 + RR42_RS01445 0.75 -0.1 325,768 + RR42_RS01445 0.75 -0.3 325,768 + RR42_RS01445 0.75 -0.3 325,769 - RR42_RS01445 0.75 -1.7
Or see this region's nucleotide sequence