Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_991

Experiment: Spectinomycin 0.05 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_990 and Pf1N1B4_991 are separated by 11 nucleotidesPf1N1B4_991 and Pf1N1B4_992 are separated by 15 nucleotidesPf1N1B4_992 and Pf1N1B4_993 are separated by 209 nucleotides Pf1N1B4_990 - Probable poly(beta-D-mannuronate) O-acetylase (EC 2.3.1.-), at 1,121,745 to 1,123,310 _990 Pf1N1B4_991 - alginate biosynthesis protein AlgJ, at 1,123,322 to 1,124,497 _991 Pf1N1B4_992 - alginate o-acetyltransferase AlgF, at 1,124,513 to 1,125,178 _992 Pf1N1B4_993 - Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22) / Mannose-6-phosphate isomerase (EC 5.3.1.8), at 1,125,388 to 1,126,839 _993 Position (kb) 1123 1124 1125Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 1122.447 kb on + strand, within Pf1N1B4_990at 1122.448 kb on - strandat 1122.462 kb on + strand, within Pf1N1B4_990at 1122.463 kb on - strand, within Pf1N1B4_990at 1122.463 kb on - strand, within Pf1N1B4_990at 1122.463 kb on - strand, within Pf1N1B4_990at 1122.463 kb on - strand, within Pf1N1B4_990at 1122.463 kb on - strand, within Pf1N1B4_990at 1122.480 kb on + strand, within Pf1N1B4_990at 1122.549 kb on + strand, within Pf1N1B4_990at 1122.549 kb on + strand, within Pf1N1B4_990at 1122.549 kb on + strand, within Pf1N1B4_990at 1122.549 kb on + strand, within Pf1N1B4_990at 1122.549 kb on + strand, within Pf1N1B4_990at 1122.550 kb on - strand, within Pf1N1B4_990at 1122.550 kb on - strand, within Pf1N1B4_990at 1122.550 kb on - strand, within Pf1N1B4_990at 1122.550 kb on - strand, within Pf1N1B4_990at 1122.550 kb on - strand, within Pf1N1B4_990at 1122.618 kb on + strand, within Pf1N1B4_990at 1122.619 kb on - strand, within Pf1N1B4_990at 1122.619 kb on - strand, within Pf1N1B4_990at 1122.619 kb on - strand, within Pf1N1B4_990at 1122.624 kb on + strand, within Pf1N1B4_990at 1122.624 kb on + strand, within Pf1N1B4_990at 1122.624 kb on + strand, within Pf1N1B4_990at 1122.624 kb on + strand, within Pf1N1B4_990at 1122.624 kb on + strand, within Pf1N1B4_990at 1122.624 kb on + strand, within Pf1N1B4_990at 1122.624 kb on + strand, within Pf1N1B4_990at 1122.624 kb on + strandat 1122.624 kb on + strand, within Pf1N1B4_990at 1122.624 kb on + strand, within Pf1N1B4_990at 1122.625 kb on - strand, within Pf1N1B4_990at 1122.625 kb on - strand, within Pf1N1B4_990at 1122.625 kb on - strandat 1122.625 kb on - strand, within Pf1N1B4_990at 1122.625 kb on - strand, within Pf1N1B4_990at 1122.625 kb on - strand, within Pf1N1B4_990at 1122.625 kb on - strand, within Pf1N1B4_990at 1122.625 kb on - strand, within Pf1N1B4_990at 1122.625 kb on - strand, within Pf1N1B4_990at 1122.625 kb on - strand, within Pf1N1B4_990at 1122.625 kb on - strand, within Pf1N1B4_990at 1122.625 kb on - strand, within Pf1N1B4_990at 1122.647 kb on + strand, within Pf1N1B4_990at 1122.648 kb on - strand, within Pf1N1B4_990at 1122.648 kb on - strand, within Pf1N1B4_990at 1122.666 kb on + strand, within Pf1N1B4_990at 1122.726 kb on + strand, within Pf1N1B4_990at 1122.726 kb on + strand, within Pf1N1B4_990at 1122.726 kb on + strand, within Pf1N1B4_990at 1122.726 kb on + strand, within Pf1N1B4_990at 1122.726 kb on + strand, within Pf1N1B4_990at 1122.726 kb on + strand, within Pf1N1B4_990at 1122.727 kb on - strand, within Pf1N1B4_990at 1122.727 kb on - strand, within Pf1N1B4_990at 1122.727 kb on - strand, within Pf1N1B4_990at 1122.727 kb on - strand, within Pf1N1B4_990at 1122.727 kb on - strand, within Pf1N1B4_990at 1122.727 kb on - strand, within Pf1N1B4_990at 1122.897 kb on + strand, within Pf1N1B4_990at 1122.897 kb on + strand, within Pf1N1B4_990at 1122.897 kb on + strand, within Pf1N1B4_990at 1122.897 kb on + strand, within Pf1N1B4_990at 1122.898 kb on - strand, within Pf1N1B4_990at 1122.898 kb on - strand, within Pf1N1B4_990at 1122.898 kb on - strand, within Pf1N1B4_990at 1122.898 kb on - strand, within Pf1N1B4_990at 1122.991 kb on - strand, within Pf1N1B4_990at 1122.991 kb on - strand, within Pf1N1B4_990at 1123.026 kb on + strand, within Pf1N1B4_990at 1123.026 kb on + strand, within Pf1N1B4_990at 1123.027 kb on - strand, within Pf1N1B4_990at 1123.027 kb on - strand, within Pf1N1B4_990at 1123.027 kb on - strand, within Pf1N1B4_990at 1123.155 kb on + strandat 1123.156 kb on - strandat 1123.192 kb on - strandat 1123.296 kb on + strandat 1123.296 kb on + strandat 1123.296 kb on + strandat 1123.296 kb on + strandat 1123.297 kb on - strandat 1123.297 kb on - strandat 1123.297 kb on - strandat 1123.322 kb on - strandat 1123.322 kb on - strandat 1123.322 kb on - strandat 1123.346 kb on + strandat 1123.346 kb on + strandat 1123.346 kb on + strandat 1123.346 kb on + strandat 1123.346 kb on + strandat 1123.346 kb on + strandat 1123.475 kb on + strand, within Pf1N1B4_991at 1123.476 kb on - strand, within Pf1N1B4_991at 1123.589 kb on + strand, within Pf1N1B4_991at 1123.589 kb on + strand, within Pf1N1B4_991at 1123.590 kb on - strand, within Pf1N1B4_991at 1123.590 kb on - strand, within Pf1N1B4_991at 1123.646 kb on + strand, within Pf1N1B4_991at 1123.646 kb on + strand, within Pf1N1B4_991at 1123.646 kb on + strand, within Pf1N1B4_991at 1123.646 kb on + strand, within Pf1N1B4_991at 1123.647 kb on - strand, within Pf1N1B4_991at 1123.647 kb on - strand, within Pf1N1B4_991at 1123.692 kb on + strandat 1123.692 kb on + strand, within Pf1N1B4_991at 1123.692 kb on + strand, within Pf1N1B4_991at 1123.692 kb on + strand, within Pf1N1B4_991at 1123.693 kb on - strand, within Pf1N1B4_991at 1123.693 kb on - strand, within Pf1N1B4_991at 1123.693 kb on - strand, within Pf1N1B4_991at 1123.693 kb on - strandat 1123.693 kb on - strand, within Pf1N1B4_991at 1123.769 kb on - strand, within Pf1N1B4_991at 1123.840 kb on - strand, within Pf1N1B4_991at 1124.189 kb on + strand, within Pf1N1B4_991at 1124.189 kb on + strand, within Pf1N1B4_991at 1124.189 kb on + strand, within Pf1N1B4_991at 1124.189 kb on + strand, within Pf1N1B4_991at 1124.190 kb on - strand, within Pf1N1B4_991at 1124.190 kb on - strand, within Pf1N1B4_991at 1124.300 kb on + strand, within Pf1N1B4_991at 1124.367 kb on - strand, within Pf1N1B4_991at 1124.422 kb on + strandat 1124.422 kb on + strandat 1124.422 kb on + strandat 1124.423 kb on - strandat 1124.423 kb on - strandat 1124.451 kb on + strandat 1124.452 kb on - strandat 1124.464 kb on + strandat 1124.464 kb on + strandat 1124.506 kb on + strandat 1124.506 kb on + strandat 1124.507 kb on - strandat 1124.524 kb on + strandat 1124.524 kb on + strandat 1124.525 kb on - strandat 1124.527 kb on + strandat 1124.527 kb on + strandat 1124.528 kb on - strandat 1124.555 kb on + strandat 1124.669 kb on + strand, within Pf1N1B4_992at 1124.669 kb on + strand, within Pf1N1B4_992at 1124.670 kb on - strand, within Pf1N1B4_992at 1124.747 kb on + strand, within Pf1N1B4_992at 1124.828 kb on + strand, within Pf1N1B4_992at 1124.829 kb on - strand, within Pf1N1B4_992at 1124.831 kb on + strand, within Pf1N1B4_992at 1124.831 kb on + strand, within Pf1N1B4_992at 1124.831 kb on + strand, within Pf1N1B4_992at 1124.831 kb on + strand, within Pf1N1B4_992at 1124.831 kb on + strand, within Pf1N1B4_992at 1124.831 kb on + strand, within Pf1N1B4_992at 1124.831 kb on + strand, within Pf1N1B4_992at 1124.831 kb on + strand, within Pf1N1B4_992at 1124.831 kb on + strand, within Pf1N1B4_992at 1124.831 kb on + strand, within Pf1N1B4_992at 1124.831 kb on + strand, within Pf1N1B4_992at 1124.831 kb on + strand, within Pf1N1B4_992at 1124.832 kb on - strand, within Pf1N1B4_992at 1124.832 kb on - strand, within Pf1N1B4_992at 1124.832 kb on - strand, within Pf1N1B4_992at 1124.832 kb on - strand, within Pf1N1B4_992at 1124.832 kb on - strand, within Pf1N1B4_992at 1124.832 kb on - strand, within Pf1N1B4_992at 1125.044 kb on + strand, within Pf1N1B4_992at 1125.044 kb on + strand, within Pf1N1B4_992at 1125.045 kb on - strand, within Pf1N1B4_992at 1125.113 kb on + strandat 1125.113 kb on + strandat 1125.113 kb on + strandat 1125.113 kb on + strandat 1125.113 kb on + strandat 1125.114 kb on - strandat 1125.114 kb on - strandat 1125.114 kb on - strandat 1125.114 kb on - strandat 1125.180 kb on + strandat 1125.181 kb on - strandat 1125.181 kb on - strandat 1125.181 kb on - strandat 1125.251 kb on + strandat 1125.251 kb on + strandat 1125.251 kb on + strandat 1125.252 kb on - strandat 1125.352 kb on + strandat 1125.352 kb on + strandat 1125.352 kb on + strandat 1125.352 kb on + strandat 1125.352 kb on + strandat 1125.352 kb on + strandat 1125.353 kb on - strandat 1125.381 kb on + strandat 1125.382 kb on - strandat 1125.382 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml
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1,122,447 + Pf1N1B4_990 0.45 +0.9
1,122,448 - -0.9
1,122,462 + Pf1N1B4_990 0.46 +0.8
1,122,463 - Pf1N1B4_990 0.46 +1.0
1,122,463 - Pf1N1B4_990 0.46 +0.5
1,122,463 - Pf1N1B4_990 0.46 -2.0
1,122,463 - Pf1N1B4_990 0.46 +2.1
1,122,463 - Pf1N1B4_990 0.46 -0.5
1,122,480 + Pf1N1B4_990 0.47 -1.5
1,122,549 + Pf1N1B4_990 0.51 -0.5
1,122,549 + Pf1N1B4_990 0.51 +0.1
1,122,549 + Pf1N1B4_990 0.51 -0.1
1,122,549 + Pf1N1B4_990 0.51 +0.8
1,122,549 + Pf1N1B4_990 0.51 +0.1
1,122,550 - Pf1N1B4_990 0.51 -1.5
1,122,550 - Pf1N1B4_990 0.51 -0.6
1,122,550 - Pf1N1B4_990 0.51 -0.1
1,122,550 - Pf1N1B4_990 0.51 +1.1
1,122,550 - Pf1N1B4_990 0.51 -1.1
1,122,618 + Pf1N1B4_990 0.56 -1.2
1,122,619 - Pf1N1B4_990 0.56 -1.2
1,122,619 - Pf1N1B4_990 0.56 -0.6
1,122,619 - Pf1N1B4_990 0.56 +2.5
1,122,624 + Pf1N1B4_990 0.56 -0.5
1,122,624 + Pf1N1B4_990 0.56 -1.5
1,122,624 + Pf1N1B4_990 0.56 -1.5
1,122,624 + Pf1N1B4_990 0.56 -1.2
1,122,624 + Pf1N1B4_990 0.56 +1.8
1,122,624 + Pf1N1B4_990 0.56 -0.7
1,122,624 + Pf1N1B4_990 0.56 -0.9
1,122,624 + -0.9
1,122,624 + Pf1N1B4_990 0.56 +0.3
1,122,624 + Pf1N1B4_990 0.56 -1.5
1,122,625 - Pf1N1B4_990 0.56 +0.3
1,122,625 - Pf1N1B4_990 0.56 +0.1
1,122,625 - -0.5
1,122,625 - Pf1N1B4_990 0.56 -1.2
1,122,625 - Pf1N1B4_990 0.56 -1.4
1,122,625 - Pf1N1B4_990 0.56 +0.1
1,122,625 - Pf1N1B4_990 0.56 +1.1
1,122,625 - Pf1N1B4_990 0.56 -0.5
1,122,625 - Pf1N1B4_990 0.56 -0.6
1,122,625 - Pf1N1B4_990 0.56 -0.9
1,122,625 - Pf1N1B4_990 0.56 +0.5
1,122,625 - Pf1N1B4_990 0.56 -1.2
1,122,647 + Pf1N1B4_990 0.58 -0.5
1,122,648 - Pf1N1B4_990 0.58 -1.7
1,122,648 - Pf1N1B4_990 0.58 +0.9
1,122,666 + Pf1N1B4_990 0.59 -0.8
1,122,726 + Pf1N1B4_990 0.63 +0.2
1,122,726 + Pf1N1B4_990 0.63 +1.1
1,122,726 + Pf1N1B4_990 0.63 -0.5
1,122,726 + Pf1N1B4_990 0.63 -0.9
1,122,726 + Pf1N1B4_990 0.63 +0.4
1,122,726 + Pf1N1B4_990 0.63 -0.9
1,122,727 - Pf1N1B4_990 0.63 -0.1
1,122,727 - Pf1N1B4_990 0.63 +1.8
1,122,727 - Pf1N1B4_990 0.63 -0.6
1,122,727 - Pf1N1B4_990 0.63 +2.1
1,122,727 - Pf1N1B4_990 0.63 -1.9
1,122,727 - Pf1N1B4_990 0.63 -1.2
1,122,897 + Pf1N1B4_990 0.74 +1.1
1,122,897 + Pf1N1B4_990 0.74 +1.1
1,122,897 + Pf1N1B4_990 0.74 -0.5
1,122,897 + Pf1N1B4_990 0.74 +1.5
1,122,898 - Pf1N1B4_990 0.74 -0.5
1,122,898 - Pf1N1B4_990 0.74 -1.5
1,122,898 - Pf1N1B4_990 0.74 +0.1
1,122,898 - Pf1N1B4_990 0.74 +0.3
1,122,991 - Pf1N1B4_990 0.80 +0.3
1,122,991 - Pf1N1B4_990 0.80 +1.1
1,123,026 + Pf1N1B4_990 0.82 +0.5
1,123,026 + Pf1N1B4_990 0.82 -0.3
1,123,027 - Pf1N1B4_990 0.82 -0.6
1,123,027 - Pf1N1B4_990 0.82 +0.1
1,123,027 - Pf1N1B4_990 0.82 -0.7
1,123,155 + +2.7
1,123,156 - +1.7
1,123,192 - -1.9
1,123,296 + +1.9
1,123,296 + -0.0
1,123,296 + -0.5
1,123,296 + +0.6
1,123,297 - -1.7
1,123,297 - +1.4
1,123,297 - -1.9
1,123,322 - +0.3
1,123,322 - -0.0
1,123,322 - +0.7
1,123,346 + +0.4
1,123,346 + +1.1
1,123,346 + -1.4
1,123,346 + +0.5
1,123,346 + -2.5
1,123,346 + +2.9
1,123,475 + Pf1N1B4_991 0.13 +0.9
1,123,476 - Pf1N1B4_991 0.13 -1.0
1,123,589 + Pf1N1B4_991 0.23 +2.5
1,123,589 + Pf1N1B4_991 0.23 +1.5
1,123,590 - Pf1N1B4_991 0.23 -1.1
1,123,590 - Pf1N1B4_991 0.23 -1.7
1,123,646 + Pf1N1B4_991 0.28 -1.6
1,123,646 + Pf1N1B4_991 0.28 -0.2
1,123,646 + Pf1N1B4_991 0.28 -0.4
1,123,646 + Pf1N1B4_991 0.28 +0.1
1,123,647 - Pf1N1B4_991 0.28 +2.7
1,123,647 - Pf1N1B4_991 0.28 +1.1
1,123,692 + -0.5
1,123,692 + Pf1N1B4_991 0.31 -1.3
1,123,692 + Pf1N1B4_991 0.31 +1.5
1,123,692 + Pf1N1B4_991 0.31 -2.3
1,123,693 - Pf1N1B4_991 0.32 -1.9
1,123,693 - Pf1N1B4_991 0.32 -0.2
1,123,693 - Pf1N1B4_991 0.32 +0.7
1,123,693 - -0.9
1,123,693 - Pf1N1B4_991 0.32 -1.2
1,123,769 - Pf1N1B4_991 0.38 -0.3
1,123,840 - Pf1N1B4_991 0.44 -0.4
1,124,189 + Pf1N1B4_991 0.74 +0.1
1,124,189 + Pf1N1B4_991 0.74 -0.6
1,124,189 + Pf1N1B4_991 0.74 +1.4
1,124,189 + Pf1N1B4_991 0.74 +1.3
1,124,190 - Pf1N1B4_991 0.74 +1.8
1,124,190 - Pf1N1B4_991 0.74 +1.1
1,124,300 + Pf1N1B4_991 0.83 +0.5
1,124,367 - Pf1N1B4_991 0.89 -0.2
1,124,422 + +0.5
1,124,422 + +0.1
1,124,422 + +0.1
1,124,423 - -0.5
1,124,423 - +0.5
1,124,451 + +1.7
1,124,452 - +1.1
1,124,464 + -1.9
1,124,464 + -0.4
1,124,506 + -1.3
1,124,506 + +1.7
1,124,507 - +0.7
1,124,524 + -0.5
1,124,524 + +0.1
1,124,525 - -0.0
1,124,527 + -0.1
1,124,527 + -0.1
1,124,528 - -0.4
1,124,555 + -1.5
1,124,669 + Pf1N1B4_992 0.23 +0.3
1,124,669 + Pf1N1B4_992 0.23 +1.0
1,124,670 - Pf1N1B4_992 0.24 -0.9
1,124,747 + Pf1N1B4_992 0.35 +4.6
1,124,828 + Pf1N1B4_992 0.47 -1.2
1,124,829 - Pf1N1B4_992 0.47 -0.5
1,124,831 + Pf1N1B4_992 0.48 +1.1
1,124,831 + Pf1N1B4_992 0.48 -0.2
1,124,831 + Pf1N1B4_992 0.48 -1.5
1,124,831 + Pf1N1B4_992 0.48 -1.9
1,124,831 + Pf1N1B4_992 0.48 +0.3
1,124,831 + Pf1N1B4_992 0.48 -0.1
1,124,831 + Pf1N1B4_992 0.48 +0.5
1,124,831 + Pf1N1B4_992 0.48 +2.7
1,124,831 + Pf1N1B4_992 0.48 +1.7
1,124,831 + Pf1N1B4_992 0.48 -0.3
1,124,831 + Pf1N1B4_992 0.48 +1.3
1,124,831 + Pf1N1B4_992 0.48 -0.3
1,124,832 - Pf1N1B4_992 0.48 -1.6
1,124,832 - Pf1N1B4_992 0.48 -0.9
1,124,832 - Pf1N1B4_992 0.48 -0.5
1,124,832 - Pf1N1B4_992 0.48 +0.5
1,124,832 - Pf1N1B4_992 0.48 -1.0
1,124,832 - Pf1N1B4_992 0.48 +0.1
1,125,044 + Pf1N1B4_992 0.80 -0.0
1,125,044 + Pf1N1B4_992 0.80 -0.5
1,125,045 - Pf1N1B4_992 0.80 +2.1
1,125,113 + +0.5
1,125,113 + -2.1
1,125,113 + +0.1
1,125,113 + +0.1
1,125,113 + -0.6
1,125,114 - -0.2
1,125,114 - -0.3
1,125,114 - +0.8
1,125,114 - -0.2
1,125,180 + +1.1
1,125,181 - -0.6
1,125,181 - -2.2
1,125,181 - +0.5
1,125,251 + +0.1
1,125,251 + +2.1
1,125,251 + +0.4
1,125,252 - -0.4
1,125,352 + +0.1
1,125,352 + +0.9
1,125,352 + +0.1
1,125,352 + -0.5
1,125,352 + +0.7
1,125,352 + +1.1
1,125,353 - -1.2
1,125,381 + +0.1
1,125,382 - +0.1
1,125,382 - +1.9

Or see this region's nucleotide sequence