Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_989 and Pf1N1B4_990 are separated by 189 nucleotides Pf1N1B4_990 and Pf1N1B4_991 are separated by 11 nucleotides
Pf1N1B4_989 - Alginate lyase precursor (EC 4.2.2.3), at 1,120,422 to 1,121,555
_989
Pf1N1B4_990 - Probable poly(beta-D-mannuronate) O-acetylase (EC 2.3.1.-), at 1,121,745 to 1,123,310
_990
Pf1N1B4_991 - alginate biosynthesis protein AlgJ, at 1,123,322 to 1,124,497
_991
Position (kb)
1121
1122
1123
1124 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1120.899 kb on + strand, within Pf1N1B4_989 at 1120.899 kb on + strand, within Pf1N1B4_989 at 1120.900 kb on - strand, within Pf1N1B4_989 at 1120.900 kb on - strand, within Pf1N1B4_989 at 1121.046 kb on + strand, within Pf1N1B4_989 at 1121.046 kb on + strand, within Pf1N1B4_989 at 1121.124 kb on + strand, within Pf1N1B4_989 at 1121.124 kb on + strand, within Pf1N1B4_989 at 1121.124 kb on + strand, within Pf1N1B4_989 at 1121.124 kb on + strand, within Pf1N1B4_989 at 1121.124 kb on + strand, within Pf1N1B4_989 at 1121.124 kb on + strand, within Pf1N1B4_989 at 1121.125 kb on - strand, within Pf1N1B4_989 at 1121.202 kb on + strand, within Pf1N1B4_989 at 1121.211 kb on + strand, within Pf1N1B4_989 at 1121.211 kb on + strand, within Pf1N1B4_989 at 1121.211 kb on + strand, within Pf1N1B4_989 at 1121.211 kb on + strand, within Pf1N1B4_989 at 1121.211 kb on + strand, within Pf1N1B4_989 at 1121.211 kb on + strand, within Pf1N1B4_989 at 1121.212 kb on - strand, within Pf1N1B4_989 at 1121.212 kb on - strand, within Pf1N1B4_989 at 1121.212 kb on - strand, within Pf1N1B4_989 at 1121.212 kb on - strand, within Pf1N1B4_989 at 1121.360 kb on + strand, within Pf1N1B4_989 at 1121.360 kb on + strand, within Pf1N1B4_989 at 1121.430 kb on + strand, within Pf1N1B4_989 at 1121.430 kb on + strand, within Pf1N1B4_989 at 1121.430 kb on + strand, within Pf1N1B4_989 at 1121.431 kb on - strand, within Pf1N1B4_989 at 1121.431 kb on - strand, within Pf1N1B4_989 at 1121.431 kb on - strand, within Pf1N1B4_989 at 1121.431 kb on - strand, within Pf1N1B4_989 at 1121.518 kb on - strand at 1121.554 kb on - strand at 1121.672 kb on + strand at 1121.672 kb on + strand at 1121.673 kb on - strand at 1121.749 kb on + strand at 1121.749 kb on + strand at 1121.749 kb on + strand at 1121.750 kb on - strand at 1121.750 kb on - strand at 1121.750 kb on - strand at 1121.802 kb on + strand at 1121.802 kb on + strand at 1121.802 kb on + strand at 1121.802 kb on + strand at 1121.802 kb on + strand at 1121.803 kb on - strand at 1121.803 kb on - strand at 1121.803 kb on - strand at 1121.803 kb on - strand at 1121.805 kb on + strand at 1121.806 kb on - strand at 1121.806 kb on - strand at 1121.806 kb on - strand at 1121.806 kb on - strand at 1121.806 kb on - strand at 1121.806 kb on - strand at 1121.806 kb on - strand at 1121.806 kb on - strand at 1121.853 kb on + strand at 1121.853 kb on + strand at 1121.853 kb on + strand at 1121.853 kb on + strand at 1121.853 kb on + strand at 1121.854 kb on - strand at 1121.854 kb on - strand at 1121.854 kb on - strand at 1121.854 kb on - strand at 1121.854 kb on - strand at 1121.863 kb on - strand at 1121.863 kb on - strand at 1121.919 kb on + strand, within Pf1N1B4_990 at 1121.919 kb on + strand, within Pf1N1B4_990 at 1121.920 kb on - strand, within Pf1N1B4_990 at 1122.028 kb on - strand, within Pf1N1B4_990 at 1122.129 kb on + strand, within Pf1N1B4_990 at 1122.129 kb on + strand, within Pf1N1B4_990 at 1122.130 kb on - strand, within Pf1N1B4_990 at 1122.130 kb on - strand, within Pf1N1B4_990 at 1122.130 kb on - strand, within Pf1N1B4_990 at 1122.150 kb on + strand, within Pf1N1B4_990 at 1122.151 kb on - strand, within Pf1N1B4_990 at 1122.151 kb on - strand, within Pf1N1B4_990 at 1122.151 kb on - strand, within Pf1N1B4_990 at 1122.151 kb on - strand, within Pf1N1B4_990 at 1122.151 kb on - strand, within Pf1N1B4_990 at 1122.151 kb on - strand, within Pf1N1B4_990 at 1122.151 kb on - strand, within Pf1N1B4_990 at 1122.151 kb on - strand, within Pf1N1B4_990 at 1122.166 kb on - strand, within Pf1N1B4_990 at 1122.447 kb on + strand, within Pf1N1B4_990 at 1122.448 kb on - strand at 1122.462 kb on + strand, within Pf1N1B4_990 at 1122.463 kb on - strand, within Pf1N1B4_990 at 1122.463 kb on - strand, within Pf1N1B4_990 at 1122.463 kb on - strand, within Pf1N1B4_990 at 1122.463 kb on - strand, within Pf1N1B4_990 at 1122.463 kb on - strand, within Pf1N1B4_990 at 1122.480 kb on + strand, within Pf1N1B4_990 at 1122.549 kb on + strand, within Pf1N1B4_990 at 1122.549 kb on + strand, within Pf1N1B4_990 at 1122.549 kb on + strand, within Pf1N1B4_990 at 1122.549 kb on + strand, within Pf1N1B4_990 at 1122.549 kb on + strand, within Pf1N1B4_990 at 1122.550 kb on - strand, within Pf1N1B4_990 at 1122.550 kb on - strand, within Pf1N1B4_990 at 1122.550 kb on - strand, within Pf1N1B4_990 at 1122.550 kb on - strand, within Pf1N1B4_990 at 1122.550 kb on - strand, within Pf1N1B4_990 at 1122.618 kb on + strand, within Pf1N1B4_990 at 1122.619 kb on - strand, within Pf1N1B4_990 at 1122.619 kb on - strand, within Pf1N1B4_990 at 1122.619 kb on - strand, within Pf1N1B4_990 at 1122.624 kb on + strand, within Pf1N1B4_990 at 1122.624 kb on + strand, within Pf1N1B4_990 at 1122.624 kb on + strand, within Pf1N1B4_990 at 1122.624 kb on + strand, within Pf1N1B4_990 at 1122.624 kb on + strand, within Pf1N1B4_990 at 1122.624 kb on + strand, within Pf1N1B4_990 at 1122.624 kb on + strand, within Pf1N1B4_990 at 1122.624 kb on + strand at 1122.624 kb on + strand, within Pf1N1B4_990 at 1122.624 kb on + strand, within Pf1N1B4_990 at 1122.625 kb on - strand, within Pf1N1B4_990 at 1122.625 kb on - strand, within Pf1N1B4_990 at 1122.625 kb on - strand at 1122.625 kb on - strand, within Pf1N1B4_990 at 1122.625 kb on - strand, within Pf1N1B4_990 at 1122.625 kb on - strand, within Pf1N1B4_990 at 1122.625 kb on - strand, within Pf1N1B4_990 at 1122.625 kb on - strand, within Pf1N1B4_990 at 1122.625 kb on - strand, within Pf1N1B4_990 at 1122.625 kb on - strand, within Pf1N1B4_990 at 1122.625 kb on - strand, within Pf1N1B4_990 at 1122.625 kb on - strand, within Pf1N1B4_990 at 1122.647 kb on + strand, within Pf1N1B4_990 at 1122.648 kb on - strand, within Pf1N1B4_990 at 1122.648 kb on - strand, within Pf1N1B4_990 at 1122.666 kb on + strand, within Pf1N1B4_990 at 1122.726 kb on + strand, within Pf1N1B4_990 at 1122.726 kb on + strand, within Pf1N1B4_990 at 1122.726 kb on + strand, within Pf1N1B4_990 at 1122.726 kb on + strand, within Pf1N1B4_990 at 1122.726 kb on + strand, within Pf1N1B4_990 at 1122.726 kb on + strand, within Pf1N1B4_990 at 1122.727 kb on - strand, within Pf1N1B4_990 at 1122.727 kb on - strand, within Pf1N1B4_990 at 1122.727 kb on - strand, within Pf1N1B4_990 at 1122.727 kb on - strand, within Pf1N1B4_990 at 1122.727 kb on - strand, within Pf1N1B4_990 at 1122.727 kb on - strand, within Pf1N1B4_990 at 1122.897 kb on + strand, within Pf1N1B4_990 at 1122.897 kb on + strand, within Pf1N1B4_990 at 1122.897 kb on + strand, within Pf1N1B4_990 at 1122.897 kb on + strand, within Pf1N1B4_990 at 1122.898 kb on - strand, within Pf1N1B4_990 at 1122.898 kb on - strand, within Pf1N1B4_990 at 1122.898 kb on - strand, within Pf1N1B4_990 at 1122.898 kb on - strand, within Pf1N1B4_990 at 1122.991 kb on - strand, within Pf1N1B4_990 at 1122.991 kb on - strand, within Pf1N1B4_990 at 1123.026 kb on + strand, within Pf1N1B4_990 at 1123.026 kb on + strand, within Pf1N1B4_990 at 1123.027 kb on - strand, within Pf1N1B4_990 at 1123.027 kb on - strand, within Pf1N1B4_990 at 1123.027 kb on - strand, within Pf1N1B4_990 at 1123.155 kb on + strand at 1123.156 kb on - strand at 1123.192 kb on - strand at 1123.296 kb on + strand at 1123.296 kb on + strand at 1123.296 kb on + strand at 1123.296 kb on + strand at 1123.297 kb on - strand at 1123.297 kb on - strand at 1123.297 kb on - strand at 1123.322 kb on - strand at 1123.322 kb on - strand at 1123.322 kb on - strand at 1123.346 kb on + strand at 1123.346 kb on + strand at 1123.346 kb on + strand at 1123.346 kb on + strand at 1123.346 kb on + strand at 1123.346 kb on + strand at 1123.475 kb on + strand, within Pf1N1B4_991 at 1123.476 kb on - strand, within Pf1N1B4_991 at 1123.589 kb on + strand, within Pf1N1B4_991 at 1123.589 kb on + strand, within Pf1N1B4_991 at 1123.590 kb on - strand, within Pf1N1B4_991 at 1123.590 kb on - strand, within Pf1N1B4_991 at 1123.646 kb on + strand, within Pf1N1B4_991 at 1123.646 kb on + strand, within Pf1N1B4_991 at 1123.646 kb on + strand, within Pf1N1B4_991 at 1123.646 kb on + strand, within Pf1N1B4_991 at 1123.647 kb on - strand, within Pf1N1B4_991 at 1123.647 kb on - strand, within Pf1N1B4_991 at 1123.692 kb on + strand at 1123.692 kb on + strand, within Pf1N1B4_991 at 1123.692 kb on + strand, within Pf1N1B4_991 at 1123.692 kb on + strand, within Pf1N1B4_991 at 1123.693 kb on - strand, within Pf1N1B4_991 at 1123.693 kb on - strand, within Pf1N1B4_991 at 1123.693 kb on - strand, within Pf1N1B4_991 at 1123.693 kb on - strand at 1123.693 kb on - strand, within Pf1N1B4_991 at 1123.769 kb on - strand, within Pf1N1B4_991 at 1123.840 kb on - strand, within Pf1N1B4_991 at 1124.189 kb on + strand, within Pf1N1B4_991 at 1124.189 kb on + strand, within Pf1N1B4_991 at 1124.189 kb on + strand, within Pf1N1B4_991 at 1124.189 kb on + strand, within Pf1N1B4_991 at 1124.190 kb on - strand, within Pf1N1B4_991 at 1124.190 kb on - strand, within Pf1N1B4_991 at 1124.300 kb on + strand, within Pf1N1B4_991
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 1,120,899 + Pf1N1B4_989 0.42 +0.1 1,120,899 + Pf1N1B4_989 0.42 -1.2 1,120,900 - Pf1N1B4_989 0.42 +2.1 1,120,900 - Pf1N1B4_989 0.42 +0.2 1,121,046 + Pf1N1B4_989 0.55 -1.7 1,121,046 + Pf1N1B4_989 0.55 +0.5 1,121,124 + Pf1N1B4_989 0.62 +0.1 1,121,124 + Pf1N1B4_989 0.62 -2.0 1,121,124 + Pf1N1B4_989 0.62 +0.7 1,121,124 + Pf1N1B4_989 0.62 +0.9 1,121,124 + Pf1N1B4_989 0.62 -1.3 1,121,124 + Pf1N1B4_989 0.62 +0.1 1,121,125 - Pf1N1B4_989 0.62 +0.8 1,121,202 + Pf1N1B4_989 0.69 -1.0 1,121,211 + Pf1N1B4_989 0.70 +0.4 1,121,211 + Pf1N1B4_989 0.70 -0.4 1,121,211 + Pf1N1B4_989 0.70 +0.1 1,121,211 + Pf1N1B4_989 0.70 +0.8 1,121,211 + Pf1N1B4_989 0.70 -2.7 1,121,211 + Pf1N1B4_989 0.70 -0.3 1,121,212 - Pf1N1B4_989 0.70 +1.5 1,121,212 - Pf1N1B4_989 0.70 -0.9 1,121,212 - Pf1N1B4_989 0.70 -1.9 1,121,212 - Pf1N1B4_989 0.70 -1.5 1,121,360 + Pf1N1B4_989 0.83 -0.6 1,121,360 + Pf1N1B4_989 0.83 +0.5 1,121,430 + Pf1N1B4_989 0.89 -0.3 1,121,430 + Pf1N1B4_989 0.89 -1.2 1,121,430 + Pf1N1B4_989 0.89 +0.3 1,121,431 - Pf1N1B4_989 0.89 +1.5 1,121,431 - Pf1N1B4_989 0.89 +1.1 1,121,431 - Pf1N1B4_989 0.89 +0.1 1,121,431 - Pf1N1B4_989 0.89 -2.0 1,121,518 - +0.6 1,121,554 - -0.9 1,121,672 + -0.7 1,121,672 + +0.8 1,121,673 - -0.8 1,121,749 + +1.5 1,121,749 + -2.0 1,121,749 + -0.5 1,121,750 - +0.9 1,121,750 - +0.8 1,121,750 - +1.7 1,121,802 + +0.1 1,121,802 + +0.3 1,121,802 + -2.0 1,121,802 + +0.3 1,121,802 + -1.7 1,121,803 - +0.5 1,121,803 - +2.4 1,121,803 - -0.3 1,121,803 - -0.3 1,121,805 + +1.1 1,121,806 - +0.5 1,121,806 - -1.2 1,121,806 - +0.4 1,121,806 - -1.9 1,121,806 - +0.5 1,121,806 - -1.0 1,121,806 - +1.1 1,121,806 - +0.7 1,121,853 + -0.2 1,121,853 + +0.7 1,121,853 + +0.7 1,121,853 + +0.5 1,121,853 + +1.7 1,121,854 - -0.5 1,121,854 - +4.1 1,121,854 - +0.1 1,121,854 - +0.4 1,121,854 - +0.4 1,121,863 - -0.7 1,121,863 - -1.3 1,121,919 + Pf1N1B4_990 0.11 +0.3 1,121,919 + Pf1N1B4_990 0.11 -1.0 1,121,920 - Pf1N1B4_990 0.11 +0.3 1,122,028 - Pf1N1B4_990 0.18 -1.5 1,122,129 + Pf1N1B4_990 0.25 +1.1 1,122,129 + Pf1N1B4_990 0.25 +2.3 1,122,130 - Pf1N1B4_990 0.25 +0.0 1,122,130 - Pf1N1B4_990 0.25 -0.4 1,122,130 - Pf1N1B4_990 0.25 +0.5 1,122,150 + Pf1N1B4_990 0.26 +0.1 1,122,151 - Pf1N1B4_990 0.26 +0.0 1,122,151 - Pf1N1B4_990 0.26 +0.0 1,122,151 - Pf1N1B4_990 0.26 +0.7 1,122,151 - Pf1N1B4_990 0.26 +0.9 1,122,151 - Pf1N1B4_990 0.26 +1.1 1,122,151 - Pf1N1B4_990 0.26 -1.0 1,122,151 - Pf1N1B4_990 0.26 +2.5 1,122,151 - Pf1N1B4_990 0.26 +0.8 1,122,166 - Pf1N1B4_990 0.27 -0.9 1,122,447 + Pf1N1B4_990 0.45 +0.9 1,122,448 - -0.9 1,122,462 + Pf1N1B4_990 0.46 +0.8 1,122,463 - Pf1N1B4_990 0.46 +1.0 1,122,463 - Pf1N1B4_990 0.46 +0.5 1,122,463 - Pf1N1B4_990 0.46 -2.0 1,122,463 - Pf1N1B4_990 0.46 +2.1 1,122,463 - Pf1N1B4_990 0.46 -0.5 1,122,480 + Pf1N1B4_990 0.47 -1.5 1,122,549 + Pf1N1B4_990 0.51 -0.5 1,122,549 + Pf1N1B4_990 0.51 +0.1 1,122,549 + Pf1N1B4_990 0.51 -0.1 1,122,549 + Pf1N1B4_990 0.51 +0.8 1,122,549 + Pf1N1B4_990 0.51 +0.1 1,122,550 - Pf1N1B4_990 0.51 -1.5 1,122,550 - Pf1N1B4_990 0.51 -0.6 1,122,550 - Pf1N1B4_990 0.51 -0.1 1,122,550 - Pf1N1B4_990 0.51 +1.1 1,122,550 - Pf1N1B4_990 0.51 -1.1 1,122,618 + Pf1N1B4_990 0.56 -1.2 1,122,619 - Pf1N1B4_990 0.56 -1.2 1,122,619 - Pf1N1B4_990 0.56 -0.6 1,122,619 - Pf1N1B4_990 0.56 +2.5 1,122,624 + Pf1N1B4_990 0.56 -0.5 1,122,624 + Pf1N1B4_990 0.56 -1.5 1,122,624 + Pf1N1B4_990 0.56 -1.5 1,122,624 + Pf1N1B4_990 0.56 -1.2 1,122,624 + Pf1N1B4_990 0.56 +1.8 1,122,624 + Pf1N1B4_990 0.56 -0.7 1,122,624 + Pf1N1B4_990 0.56 -0.9 1,122,624 + -0.9 1,122,624 + Pf1N1B4_990 0.56 +0.3 1,122,624 + Pf1N1B4_990 0.56 -1.5 1,122,625 - Pf1N1B4_990 0.56 +0.3 1,122,625 - Pf1N1B4_990 0.56 +0.1 1,122,625 - -0.5 1,122,625 - Pf1N1B4_990 0.56 -1.2 1,122,625 - Pf1N1B4_990 0.56 -1.4 1,122,625 - Pf1N1B4_990 0.56 +0.1 1,122,625 - Pf1N1B4_990 0.56 +1.1 1,122,625 - Pf1N1B4_990 0.56 -0.5 1,122,625 - Pf1N1B4_990 0.56 -0.6 1,122,625 - Pf1N1B4_990 0.56 -0.9 1,122,625 - Pf1N1B4_990 0.56 +0.5 1,122,625 - Pf1N1B4_990 0.56 -1.2 1,122,647 + Pf1N1B4_990 0.58 -0.5 1,122,648 - Pf1N1B4_990 0.58 -1.7 1,122,648 - Pf1N1B4_990 0.58 +0.9 1,122,666 + Pf1N1B4_990 0.59 -0.8 1,122,726 + Pf1N1B4_990 0.63 +0.2 1,122,726 + Pf1N1B4_990 0.63 +1.1 1,122,726 + Pf1N1B4_990 0.63 -0.5 1,122,726 + Pf1N1B4_990 0.63 -0.9 1,122,726 + Pf1N1B4_990 0.63 +0.4 1,122,726 + Pf1N1B4_990 0.63 -0.9 1,122,727 - Pf1N1B4_990 0.63 -0.1 1,122,727 - Pf1N1B4_990 0.63 +1.8 1,122,727 - Pf1N1B4_990 0.63 -0.6 1,122,727 - Pf1N1B4_990 0.63 +2.1 1,122,727 - Pf1N1B4_990 0.63 -1.9 1,122,727 - Pf1N1B4_990 0.63 -1.2 1,122,897 + Pf1N1B4_990 0.74 +1.1 1,122,897 + Pf1N1B4_990 0.74 +1.1 1,122,897 + Pf1N1B4_990 0.74 -0.5 1,122,897 + Pf1N1B4_990 0.74 +1.5 1,122,898 - Pf1N1B4_990 0.74 -0.5 1,122,898 - Pf1N1B4_990 0.74 -1.5 1,122,898 - Pf1N1B4_990 0.74 +0.1 1,122,898 - Pf1N1B4_990 0.74 +0.3 1,122,991 - Pf1N1B4_990 0.80 +0.3 1,122,991 - Pf1N1B4_990 0.80 +1.1 1,123,026 + Pf1N1B4_990 0.82 +0.5 1,123,026 + Pf1N1B4_990 0.82 -0.3 1,123,027 - Pf1N1B4_990 0.82 -0.6 1,123,027 - Pf1N1B4_990 0.82 +0.1 1,123,027 - Pf1N1B4_990 0.82 -0.7 1,123,155 + +2.7 1,123,156 - +1.7 1,123,192 - -1.9 1,123,296 + +1.9 1,123,296 + -0.0 1,123,296 + -0.5 1,123,296 + +0.6 1,123,297 - -1.7 1,123,297 - +1.4 1,123,297 - -1.9 1,123,322 - +0.3 1,123,322 - -0.0 1,123,322 - +0.7 1,123,346 + +0.4 1,123,346 + +1.1 1,123,346 + -1.4 1,123,346 + +0.5 1,123,346 + -2.5 1,123,346 + +2.9 1,123,475 + Pf1N1B4_991 0.13 +0.9 1,123,476 - Pf1N1B4_991 0.13 -1.0 1,123,589 + Pf1N1B4_991 0.23 +2.5 1,123,589 + Pf1N1B4_991 0.23 +1.5 1,123,590 - Pf1N1B4_991 0.23 -1.1 1,123,590 - Pf1N1B4_991 0.23 -1.7 1,123,646 + Pf1N1B4_991 0.28 -1.6 1,123,646 + Pf1N1B4_991 0.28 -0.2 1,123,646 + Pf1N1B4_991 0.28 -0.4 1,123,646 + Pf1N1B4_991 0.28 +0.1 1,123,647 - Pf1N1B4_991 0.28 +2.7 1,123,647 - Pf1N1B4_991 0.28 +1.1 1,123,692 + -0.5 1,123,692 + Pf1N1B4_991 0.31 -1.3 1,123,692 + Pf1N1B4_991 0.31 +1.5 1,123,692 + Pf1N1B4_991 0.31 -2.3 1,123,693 - Pf1N1B4_991 0.32 -1.9 1,123,693 - Pf1N1B4_991 0.32 -0.2 1,123,693 - Pf1N1B4_991 0.32 +0.7 1,123,693 - -0.9 1,123,693 - Pf1N1B4_991 0.32 -1.2 1,123,769 - Pf1N1B4_991 0.38 -0.3 1,123,840 - Pf1N1B4_991 0.44 -0.4 1,124,189 + Pf1N1B4_991 0.74 +0.1 1,124,189 + Pf1N1B4_991 0.74 -0.6 1,124,189 + Pf1N1B4_991 0.74 +1.4 1,124,189 + Pf1N1B4_991 0.74 +1.3 1,124,190 - Pf1N1B4_991 0.74 +1.8 1,124,190 - Pf1N1B4_991 0.74 +1.1 1,124,300 + Pf1N1B4_991 0.83 +0.5
Or see this region's nucleotide sequence