Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_986 and Pf1N1B4_987 are separated by 26 nucleotides Pf1N1B4_987 and Pf1N1B4_988 are separated by 10 nucleotides
Pf1N1B4_986 - outer membrane protein AlgE, at 1,115,836 to 1,117,323
_986
Pf1N1B4_987 - Poly(beta-D-mannuronate) C5 epimerase precursor (EC 5.1.3.-), at 1,117,350 to 1,118,939
_987
Pf1N1B4_988 - Alginate biosynthesis protein AlgX, at 1,118,950 to 1,120,410
_988
Position (kb)
1117
1118
1119 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1116.607 kb on + strand, within Pf1N1B4_986 at 1116.607 kb on + strand, within Pf1N1B4_986 at 1116.608 kb on - strand, within Pf1N1B4_986 at 1116.637 kb on + strand, within Pf1N1B4_986 at 1116.637 kb on + strand, within Pf1N1B4_986 at 1116.756 kb on + strand, within Pf1N1B4_986 at 1116.806 kb on - strand, within Pf1N1B4_986 at 1116.827 kb on - strand, within Pf1N1B4_986 at 1116.863 kb on - strand, within Pf1N1B4_986 at 1116.971 kb on - strand, within Pf1N1B4_986 at 1116.988 kb on + strand, within Pf1N1B4_986 at 1116.988 kb on + strand at 1116.988 kb on + strand, within Pf1N1B4_986 at 1116.988 kb on + strand, within Pf1N1B4_986 at 1116.988 kb on + strand, within Pf1N1B4_986 at 1116.989 kb on - strand, within Pf1N1B4_986 at 1116.989 kb on - strand, within Pf1N1B4_986 at 1116.989 kb on - strand, within Pf1N1B4_986 at 1117.159 kb on + strand, within Pf1N1B4_986 at 1117.159 kb on + strand, within Pf1N1B4_986 at 1117.159 kb on + strand, within Pf1N1B4_986 at 1117.159 kb on + strand, within Pf1N1B4_986 at 1117.160 kb on - strand, within Pf1N1B4_986 at 1117.160 kb on - strand, within Pf1N1B4_986 at 1117.160 kb on - strand, within Pf1N1B4_986 at 1117.160 kb on - strand, within Pf1N1B4_986 at 1117.160 kb on - strand, within Pf1N1B4_986 at 1117.160 kb on - strand, within Pf1N1B4_986 at 1117.262 kb on - strand at 1117.283 kb on - strand at 1117.500 kb on + strand at 1117.501 kb on - strand at 1117.501 kb on - strand at 1117.501 kb on - strand at 1117.501 kb on - strand at 1117.570 kb on - strand, within Pf1N1B4_987 at 1117.744 kb on - strand, within Pf1N1B4_987 at 1117.744 kb on - strand, within Pf1N1B4_987 at 1117.744 kb on - strand, within Pf1N1B4_987 at 1117.774 kb on + strand, within Pf1N1B4_987 at 1117.785 kb on + strand, within Pf1N1B4_987 at 1117.786 kb on - strand, within Pf1N1B4_987 at 1117.786 kb on - strand, within Pf1N1B4_987 at 1118.047 kb on - strand, within Pf1N1B4_987 at 1118.047 kb on - strand, within Pf1N1B4_987 at 1118.047 kb on - strand, within Pf1N1B4_987 at 1118.090 kb on + strand, within Pf1N1B4_987 at 1118.090 kb on + strand, within Pf1N1B4_987 at 1118.090 kb on + strand, within Pf1N1B4_987 at 1118.091 kb on - strand at 1118.091 kb on - strand, within Pf1N1B4_987 at 1118.091 kb on - strand, within Pf1N1B4_987 at 1118.091 kb on - strand at 1118.091 kb on - strand, within Pf1N1B4_987 at 1118.091 kb on - strand, within Pf1N1B4_987 at 1118.108 kb on + strand, within Pf1N1B4_987 at 1118.108 kb on + strand, within Pf1N1B4_987 at 1118.109 kb on - strand, within Pf1N1B4_987 at 1118.209 kb on - strand, within Pf1N1B4_987 at 1118.214 kb on + strand, within Pf1N1B4_987 at 1118.250 kb on + strand, within Pf1N1B4_987 at 1118.250 kb on + strand, within Pf1N1B4_987 at 1118.250 kb on + strand, within Pf1N1B4_987 at 1118.250 kb on + strand, within Pf1N1B4_987 at 1118.250 kb on + strand, within Pf1N1B4_987 at 1118.251 kb on - strand, within Pf1N1B4_987 at 1118.251 kb on - strand, within Pf1N1B4_987 at 1118.325 kb on + strand, within Pf1N1B4_987 at 1118.325 kb on + strand, within Pf1N1B4_987 at 1118.354 kb on + strand, within Pf1N1B4_987 at 1118.355 kb on - strand, within Pf1N1B4_987 at 1118.355 kb on - strand, within Pf1N1B4_987 at 1118.355 kb on - strand, within Pf1N1B4_987 at 1118.355 kb on - strand, within Pf1N1B4_987 at 1118.355 kb on - strand, within Pf1N1B4_987 at 1118.355 kb on - strand, within Pf1N1B4_987 at 1118.400 kb on + strand, within Pf1N1B4_987 at 1118.436 kb on - strand, within Pf1N1B4_987 at 1118.461 kb on - strand, within Pf1N1B4_987 at 1118.472 kb on + strand, within Pf1N1B4_987 at 1118.473 kb on - strand, within Pf1N1B4_987 at 1118.473 kb on - strand, within Pf1N1B4_987 at 1118.502 kb on + strand at 1118.502 kb on + strand, within Pf1N1B4_987 at 1118.503 kb on - strand, within Pf1N1B4_987 at 1118.567 kb on + strand at 1118.634 kb on + strand, within Pf1N1B4_987 at 1118.784 kb on + strand at 1118.785 kb on - strand at 1118.944 kb on - strand at 1118.949 kb on + strand at 1118.950 kb on - strand at 1118.973 kb on - strand at 1118.973 kb on - strand at 1119.048 kb on - strand at 1119.115 kb on + strand, within Pf1N1B4_988 at 1119.115 kb on + strand, within Pf1N1B4_988 at 1119.115 kb on + strand, within Pf1N1B4_988 at 1119.116 kb on - strand, within Pf1N1B4_988 at 1119.116 kb on - strand, within Pf1N1B4_988 at 1119.116 kb on - strand, within Pf1N1B4_988 at 1119.227 kb on - strand, within Pf1N1B4_988 at 1119.349 kb on + strand, within Pf1N1B4_988 at 1119.350 kb on - strand, within Pf1N1B4_988 at 1119.350 kb on - strand, within Pf1N1B4_988 at 1119.350 kb on - strand, within Pf1N1B4_988 at 1119.377 kb on - strand, within Pf1N1B4_988 at 1119.412 kb on + strand, within Pf1N1B4_988 at 1119.413 kb on - strand, within Pf1N1B4_988 at 1119.481 kb on + strand, within Pf1N1B4_988 at 1119.481 kb on + strand, within Pf1N1B4_988 at 1119.482 kb on - strand, within Pf1N1B4_988 at 1119.482 kb on - strand, within Pf1N1B4_988 at 1119.482 kb on - strand, within Pf1N1B4_988 at 1119.511 kb on + strand, within Pf1N1B4_988 at 1119.603 kb on + strand, within Pf1N1B4_988 at 1119.603 kb on + strand, within Pf1N1B4_988 at 1119.603 kb on + strand, within Pf1N1B4_988 at 1119.603 kb on + strand, within Pf1N1B4_988 at 1119.604 kb on - strand, within Pf1N1B4_988 at 1119.635 kb on + strand at 1119.635 kb on + strand, within Pf1N1B4_988 at 1119.682 kb on + strand, within Pf1N1B4_988 at 1119.682 kb on + strand, within Pf1N1B4_988 at 1119.683 kb on - strand, within Pf1N1B4_988 at 1119.683 kb on - strand, within Pf1N1B4_988 at 1119.683 kb on - strand, within Pf1N1B4_988 at 1119.683 kb on - strand, within Pf1N1B4_988 at 1119.683 kb on - strand, within Pf1N1B4_988 at 1119.683 kb on - strand, within Pf1N1B4_988 at 1119.763 kb on - strand, within Pf1N1B4_988 at 1119.796 kb on + strand, within Pf1N1B4_988 at 1119.796 kb on + strand, within Pf1N1B4_988 at 1119.797 kb on - strand, within Pf1N1B4_988 at 1119.797 kb on - strand, within Pf1N1B4_988 at 1119.797 kb on - strand, within Pf1N1B4_988 at 1119.797 kb on - strand, within Pf1N1B4_988 at 1119.889 kb on + strand, within Pf1N1B4_988 at 1119.890 kb on - strand, within Pf1N1B4_988
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 1,116,607 + Pf1N1B4_986 0.52 +1.7 1,116,607 + Pf1N1B4_986 0.52 -0.3 1,116,608 - Pf1N1B4_986 0.52 -1.1 1,116,637 + Pf1N1B4_986 0.54 -0.9 1,116,637 + Pf1N1B4_986 0.54 -0.2 1,116,756 + Pf1N1B4_986 0.62 +2.3 1,116,806 - Pf1N1B4_986 0.65 -0.2 1,116,827 - Pf1N1B4_986 0.67 -2.2 1,116,863 - Pf1N1B4_986 0.69 -0.9 1,116,971 - Pf1N1B4_986 0.76 +0.4 1,116,988 + Pf1N1B4_986 0.77 +0.3 1,116,988 + +2.1 1,116,988 + Pf1N1B4_986 0.77 -0.5 1,116,988 + Pf1N1B4_986 0.77 -0.7 1,116,988 + Pf1N1B4_986 0.77 -1.2 1,116,989 - Pf1N1B4_986 0.77 +1.0 1,116,989 - Pf1N1B4_986 0.77 +1.9 1,116,989 - Pf1N1B4_986 0.77 +0.1 1,117,159 + Pf1N1B4_986 0.89 +0.4 1,117,159 + Pf1N1B4_986 0.89 +0.1 1,117,159 + Pf1N1B4_986 0.89 -0.4 1,117,159 + Pf1N1B4_986 0.89 -0.2 1,117,160 - Pf1N1B4_986 0.89 -0.3 1,117,160 - Pf1N1B4_986 0.89 -0.3 1,117,160 - Pf1N1B4_986 0.89 +0.7 1,117,160 - Pf1N1B4_986 0.89 +0.9 1,117,160 - Pf1N1B4_986 0.89 +1.0 1,117,160 - Pf1N1B4_986 0.89 -1.3 1,117,262 - -1.9 1,117,283 - -0.5 1,117,500 + +1.1 1,117,501 - -2.2 1,117,501 - +3.1 1,117,501 - +0.4 1,117,501 - -1.2 1,117,570 - Pf1N1B4_987 0.14 +0.4 1,117,744 - Pf1N1B4_987 0.25 -1.5 1,117,744 - Pf1N1B4_987 0.25 +0.3 1,117,744 - Pf1N1B4_987 0.25 +1.0 1,117,774 + Pf1N1B4_987 0.27 +1.1 1,117,785 + Pf1N1B4_987 0.27 +0.5 1,117,786 - Pf1N1B4_987 0.27 +1.4 1,117,786 - Pf1N1B4_987 0.27 +0.4 1,118,047 - Pf1N1B4_987 0.44 +0.7 1,118,047 - Pf1N1B4_987 0.44 +0.7 1,118,047 - Pf1N1B4_987 0.44 -0.3 1,118,090 + Pf1N1B4_987 0.47 +0.8 1,118,090 + Pf1N1B4_987 0.47 -1.7 1,118,090 + Pf1N1B4_987 0.47 +0.7 1,118,091 - +1.1 1,118,091 - Pf1N1B4_987 0.47 -0.5 1,118,091 - Pf1N1B4_987 0.47 -0.6 1,118,091 - +0.5 1,118,091 - Pf1N1B4_987 0.47 +0.6 1,118,091 - Pf1N1B4_987 0.47 -1.4 1,118,108 + Pf1N1B4_987 0.48 -0.5 1,118,108 + Pf1N1B4_987 0.48 +0.6 1,118,109 - Pf1N1B4_987 0.48 -0.7 1,118,209 - Pf1N1B4_987 0.54 -1.3 1,118,214 + Pf1N1B4_987 0.54 +0.5 1,118,250 + Pf1N1B4_987 0.57 +0.4 1,118,250 + Pf1N1B4_987 0.57 +0.2 1,118,250 + Pf1N1B4_987 0.57 +1.1 1,118,250 + Pf1N1B4_987 0.57 -1.5 1,118,250 + Pf1N1B4_987 0.57 +2.3 1,118,251 - Pf1N1B4_987 0.57 +0.5 1,118,251 - Pf1N1B4_987 0.57 -0.6 1,118,325 + Pf1N1B4_987 0.61 -0.7 1,118,325 + Pf1N1B4_987 0.61 +0.5 1,118,354 + Pf1N1B4_987 0.63 +0.6 1,118,355 - Pf1N1B4_987 0.63 +0.4 1,118,355 - Pf1N1B4_987 0.63 -0.0 1,118,355 - Pf1N1B4_987 0.63 +0.8 1,118,355 - Pf1N1B4_987 0.63 +0.3 1,118,355 - Pf1N1B4_987 0.63 -1.0 1,118,355 - Pf1N1B4_987 0.63 -0.9 1,118,400 + Pf1N1B4_987 0.66 -0.7 1,118,436 - Pf1N1B4_987 0.68 -0.5 1,118,461 - Pf1N1B4_987 0.70 +1.1 1,118,472 + Pf1N1B4_987 0.71 +1.0 1,118,473 - Pf1N1B4_987 0.71 +0.6 1,118,473 - Pf1N1B4_987 0.71 -1.2 1,118,502 + -0.5 1,118,502 + Pf1N1B4_987 0.72 -0.9 1,118,503 - Pf1N1B4_987 0.73 +1.0 1,118,567 + +0.1 1,118,634 + Pf1N1B4_987 0.81 -2.0 1,118,784 + +1.1 1,118,785 - +0.6 1,118,944 - -1.2 1,118,949 + +0.5 1,118,950 - +0.7 1,118,973 - +0.1 1,118,973 - -1.9 1,119,048 - +0.4 1,119,115 + Pf1N1B4_988 0.11 +0.8 1,119,115 + Pf1N1B4_988 0.11 +0.2 1,119,115 + Pf1N1B4_988 0.11 +0.3 1,119,116 - Pf1N1B4_988 0.11 -0.6 1,119,116 - Pf1N1B4_988 0.11 -0.3 1,119,116 - Pf1N1B4_988 0.11 +0.4 1,119,227 - Pf1N1B4_988 0.19 -0.4 1,119,349 + Pf1N1B4_988 0.27 +0.5 1,119,350 - Pf1N1B4_988 0.27 -0.9 1,119,350 - Pf1N1B4_988 0.27 -0.0 1,119,350 - Pf1N1B4_988 0.27 -0.5 1,119,377 - Pf1N1B4_988 0.29 -0.5 1,119,412 + Pf1N1B4_988 0.32 +0.3 1,119,413 - Pf1N1B4_988 0.32 -1.3 1,119,481 + Pf1N1B4_988 0.36 +0.1 1,119,481 + Pf1N1B4_988 0.36 -0.5 1,119,482 - Pf1N1B4_988 0.36 +1.5 1,119,482 - Pf1N1B4_988 0.36 -0.2 1,119,482 - Pf1N1B4_988 0.36 +0.8 1,119,511 + Pf1N1B4_988 0.38 -0.2 1,119,603 + Pf1N1B4_988 0.45 -0.9 1,119,603 + Pf1N1B4_988 0.45 -0.2 1,119,603 + Pf1N1B4_988 0.45 +1.5 1,119,603 + Pf1N1B4_988 0.45 -0.2 1,119,604 - Pf1N1B4_988 0.45 -0.9 1,119,635 + -1.2 1,119,635 + Pf1N1B4_988 0.47 +0.8 1,119,682 + Pf1N1B4_988 0.50 -0.9 1,119,682 + Pf1N1B4_988 0.50 +2.1 1,119,683 - Pf1N1B4_988 0.50 +0.7 1,119,683 - Pf1N1B4_988 0.50 +1.5 1,119,683 - Pf1N1B4_988 0.50 -0.1 1,119,683 - Pf1N1B4_988 0.50 -0.6 1,119,683 - Pf1N1B4_988 0.50 -1.9 1,119,683 - Pf1N1B4_988 0.50 +1.1 1,119,763 - Pf1N1B4_988 0.56 +0.4 1,119,796 + Pf1N1B4_988 0.58 -1.4 1,119,796 + Pf1N1B4_988 0.58 +0.6 1,119,797 - Pf1N1B4_988 0.58 -1.0 1,119,797 - Pf1N1B4_988 0.58 +0.1 1,119,797 - Pf1N1B4_988 0.58 -0.2 1,119,797 - Pf1N1B4_988 0.58 -1.2 1,119,889 + Pf1N1B4_988 0.64 +0.8 1,119,890 - Pf1N1B4_988 0.64 -1.5
Or see this region's nucleotide sequence