Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_985 and Pf1N1B4_986 overlap by 4 nucleotides Pf1N1B4_986 and Pf1N1B4_987 are separated by 26 nucleotides
Pf1N1B4_985 - Alginate biosynthesis protein AlgK precursor, at 1,114,334 to 1,115,839
_985
Pf1N1B4_986 - outer membrane protein AlgE, at 1,115,836 to 1,117,323
_986
Pf1N1B4_987 - Poly(beta-D-mannuronate) C5 epimerase precursor (EC 5.1.3.-), at 1,117,350 to 1,118,939
_987
Position (kb)
1115
1116
1117
1118 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1114.851 kb on - strand, within Pf1N1B4_985 at 1114.851 kb on - strand, within Pf1N1B4_985 at 1114.973 kb on + strand, within Pf1N1B4_985 at 1114.973 kb on + strand, within Pf1N1B4_985 at 1114.973 kb on + strand, within Pf1N1B4_985 at 1114.974 kb on - strand, within Pf1N1B4_985 at 1114.974 kb on - strand, within Pf1N1B4_985 at 1114.974 kb on - strand, within Pf1N1B4_985 at 1114.974 kb on - strand, within Pf1N1B4_985 at 1115.039 kb on + strand at 1115.039 kb on + strand, within Pf1N1B4_985 at 1115.040 kb on - strand, within Pf1N1B4_985 at 1115.040 kb on - strand, within Pf1N1B4_985 at 1115.040 kb on - strand, within Pf1N1B4_985 at 1115.279 kb on + strand, within Pf1N1B4_985 at 1115.321 kb on + strand, within Pf1N1B4_985 at 1115.322 kb on - strand, within Pf1N1B4_985 at 1115.322 kb on - strand, within Pf1N1B4_985 at 1115.382 kb on - strand, within Pf1N1B4_985 at 1115.382 kb on - strand, within Pf1N1B4_985 at 1115.471 kb on + strand, within Pf1N1B4_985 at 1115.471 kb on + strand, within Pf1N1B4_985 at 1115.472 kb on - strand, within Pf1N1B4_985 at 1115.472 kb on - strand, within Pf1N1B4_985 at 1115.636 kb on + strand, within Pf1N1B4_985 at 1115.636 kb on + strand, within Pf1N1B4_985 at 1115.636 kb on + strand, within Pf1N1B4_985 at 1115.636 kb on + strand, within Pf1N1B4_985 at 1115.637 kb on - strand, within Pf1N1B4_985 at 1115.637 kb on - strand, within Pf1N1B4_985 at 1115.637 kb on - strand, within Pf1N1B4_985 at 1115.637 kb on - strand, within Pf1N1B4_985 at 1115.770 kb on + strand at 1115.835 kb on + strand at 1115.836 kb on - strand at 1115.836 kb on - strand at 1116.184 kb on + strand, within Pf1N1B4_986 at 1116.184 kb on + strand at 1116.185 kb on - strand at 1116.185 kb on - strand, within Pf1N1B4_986 at 1116.185 kb on - strand, within Pf1N1B4_986 at 1116.607 kb on + strand, within Pf1N1B4_986 at 1116.607 kb on + strand, within Pf1N1B4_986 at 1116.608 kb on - strand, within Pf1N1B4_986 at 1116.637 kb on + strand, within Pf1N1B4_986 at 1116.637 kb on + strand, within Pf1N1B4_986 at 1116.756 kb on + strand, within Pf1N1B4_986 at 1116.806 kb on - strand, within Pf1N1B4_986 at 1116.827 kb on - strand, within Pf1N1B4_986 at 1116.863 kb on - strand, within Pf1N1B4_986 at 1116.971 kb on - strand, within Pf1N1B4_986 at 1116.988 kb on + strand, within Pf1N1B4_986 at 1116.988 kb on + strand at 1116.988 kb on + strand, within Pf1N1B4_986 at 1116.988 kb on + strand, within Pf1N1B4_986 at 1116.988 kb on + strand, within Pf1N1B4_986 at 1116.989 kb on - strand, within Pf1N1B4_986 at 1116.989 kb on - strand, within Pf1N1B4_986 at 1116.989 kb on - strand, within Pf1N1B4_986 at 1117.159 kb on + strand, within Pf1N1B4_986 at 1117.159 kb on + strand, within Pf1N1B4_986 at 1117.159 kb on + strand, within Pf1N1B4_986 at 1117.159 kb on + strand, within Pf1N1B4_986 at 1117.160 kb on - strand, within Pf1N1B4_986 at 1117.160 kb on - strand, within Pf1N1B4_986 at 1117.160 kb on - strand, within Pf1N1B4_986 at 1117.160 kb on - strand, within Pf1N1B4_986 at 1117.160 kb on - strand, within Pf1N1B4_986 at 1117.160 kb on - strand, within Pf1N1B4_986 at 1117.262 kb on - strand at 1117.283 kb on - strand at 1117.500 kb on + strand at 1117.501 kb on - strand at 1117.501 kb on - strand at 1117.501 kb on - strand at 1117.501 kb on - strand at 1117.570 kb on - strand, within Pf1N1B4_987 at 1117.744 kb on - strand, within Pf1N1B4_987 at 1117.744 kb on - strand, within Pf1N1B4_987 at 1117.744 kb on - strand, within Pf1N1B4_987 at 1117.774 kb on + strand, within Pf1N1B4_987 at 1117.785 kb on + strand, within Pf1N1B4_987 at 1117.786 kb on - strand, within Pf1N1B4_987 at 1117.786 kb on - strand, within Pf1N1B4_987 at 1118.047 kb on - strand, within Pf1N1B4_987 at 1118.047 kb on - strand, within Pf1N1B4_987 at 1118.047 kb on - strand, within Pf1N1B4_987 at 1118.090 kb on + strand, within Pf1N1B4_987 at 1118.090 kb on + strand, within Pf1N1B4_987 at 1118.090 kb on + strand, within Pf1N1B4_987 at 1118.091 kb on - strand at 1118.091 kb on - strand, within Pf1N1B4_987 at 1118.091 kb on - strand, within Pf1N1B4_987 at 1118.091 kb on - strand at 1118.091 kb on - strand, within Pf1N1B4_987 at 1118.091 kb on - strand, within Pf1N1B4_987 at 1118.108 kb on + strand, within Pf1N1B4_987 at 1118.108 kb on + strand, within Pf1N1B4_987 at 1118.109 kb on - strand, within Pf1N1B4_987 at 1118.209 kb on - strand, within Pf1N1B4_987 at 1118.214 kb on + strand, within Pf1N1B4_987 at 1118.250 kb on + strand, within Pf1N1B4_987 at 1118.250 kb on + strand, within Pf1N1B4_987 at 1118.250 kb on + strand, within Pf1N1B4_987 at 1118.250 kb on + strand, within Pf1N1B4_987 at 1118.250 kb on + strand, within Pf1N1B4_987 at 1118.251 kb on - strand, within Pf1N1B4_987 at 1118.251 kb on - strand, within Pf1N1B4_987
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 1,114,851 - Pf1N1B4_985 0.34 +0.9 1,114,851 - Pf1N1B4_985 0.34 +0.7 1,114,973 + Pf1N1B4_985 0.42 -0.7 1,114,973 + Pf1N1B4_985 0.42 +1.5 1,114,973 + Pf1N1B4_985 0.42 +0.8 1,114,974 - Pf1N1B4_985 0.42 +0.1 1,114,974 - Pf1N1B4_985 0.42 +0.1 1,114,974 - Pf1N1B4_985 0.42 -1.2 1,114,974 - Pf1N1B4_985 0.42 +1.4 1,115,039 + -1.9 1,115,039 + Pf1N1B4_985 0.47 -0.7 1,115,040 - Pf1N1B4_985 0.47 +1.5 1,115,040 - Pf1N1B4_985 0.47 -2.7 1,115,040 - Pf1N1B4_985 0.47 +0.9 1,115,279 + Pf1N1B4_985 0.63 +0.3 1,115,321 + Pf1N1B4_985 0.66 -1.0 1,115,322 - Pf1N1B4_985 0.66 -1.7 1,115,322 - Pf1N1B4_985 0.66 +0.9 1,115,382 - Pf1N1B4_985 0.70 +0.6 1,115,382 - Pf1N1B4_985 0.70 +1.4 1,115,471 + Pf1N1B4_985 0.75 +1.1 1,115,471 + Pf1N1B4_985 0.75 +0.5 1,115,472 - Pf1N1B4_985 0.76 -2.2 1,115,472 - Pf1N1B4_985 0.76 -3.3 1,115,636 + Pf1N1B4_985 0.86 +0.4 1,115,636 + Pf1N1B4_985 0.86 +1.0 1,115,636 + Pf1N1B4_985 0.86 -0.9 1,115,636 + Pf1N1B4_985 0.86 -0.9 1,115,637 - Pf1N1B4_985 0.87 -0.1 1,115,637 - Pf1N1B4_985 0.87 -0.3 1,115,637 - Pf1N1B4_985 0.87 -0.0 1,115,637 - Pf1N1B4_985 0.87 -0.5 1,115,770 + +0.3 1,115,835 + +0.1 1,115,836 - -0.6 1,115,836 - -1.5 1,116,184 + Pf1N1B4_986 0.23 +0.9 1,116,184 + -1.2 1,116,185 - +0.7 1,116,185 - Pf1N1B4_986 0.23 -0.1 1,116,185 - Pf1N1B4_986 0.23 -0.7 1,116,607 + Pf1N1B4_986 0.52 +1.7 1,116,607 + Pf1N1B4_986 0.52 -0.3 1,116,608 - Pf1N1B4_986 0.52 -1.1 1,116,637 + Pf1N1B4_986 0.54 -0.9 1,116,637 + Pf1N1B4_986 0.54 -0.2 1,116,756 + Pf1N1B4_986 0.62 +2.3 1,116,806 - Pf1N1B4_986 0.65 -0.2 1,116,827 - Pf1N1B4_986 0.67 -2.2 1,116,863 - Pf1N1B4_986 0.69 -0.9 1,116,971 - Pf1N1B4_986 0.76 +0.4 1,116,988 + Pf1N1B4_986 0.77 +0.3 1,116,988 + +2.1 1,116,988 + Pf1N1B4_986 0.77 -0.5 1,116,988 + Pf1N1B4_986 0.77 -0.7 1,116,988 + Pf1N1B4_986 0.77 -1.2 1,116,989 - Pf1N1B4_986 0.77 +1.0 1,116,989 - Pf1N1B4_986 0.77 +1.9 1,116,989 - Pf1N1B4_986 0.77 +0.1 1,117,159 + Pf1N1B4_986 0.89 +0.4 1,117,159 + Pf1N1B4_986 0.89 +0.1 1,117,159 + Pf1N1B4_986 0.89 -0.4 1,117,159 + Pf1N1B4_986 0.89 -0.2 1,117,160 - Pf1N1B4_986 0.89 -0.3 1,117,160 - Pf1N1B4_986 0.89 -0.3 1,117,160 - Pf1N1B4_986 0.89 +0.7 1,117,160 - Pf1N1B4_986 0.89 +0.9 1,117,160 - Pf1N1B4_986 0.89 +1.0 1,117,160 - Pf1N1B4_986 0.89 -1.3 1,117,262 - -1.9 1,117,283 - -0.5 1,117,500 + +1.1 1,117,501 - -2.2 1,117,501 - +3.1 1,117,501 - +0.4 1,117,501 - -1.2 1,117,570 - Pf1N1B4_987 0.14 +0.4 1,117,744 - Pf1N1B4_987 0.25 -1.5 1,117,744 - Pf1N1B4_987 0.25 +0.3 1,117,744 - Pf1N1B4_987 0.25 +1.0 1,117,774 + Pf1N1B4_987 0.27 +1.1 1,117,785 + Pf1N1B4_987 0.27 +0.5 1,117,786 - Pf1N1B4_987 0.27 +1.4 1,117,786 - Pf1N1B4_987 0.27 +0.4 1,118,047 - Pf1N1B4_987 0.44 +0.7 1,118,047 - Pf1N1B4_987 0.44 +0.7 1,118,047 - Pf1N1B4_987 0.44 -0.3 1,118,090 + Pf1N1B4_987 0.47 +0.8 1,118,090 + Pf1N1B4_987 0.47 -1.7 1,118,090 + Pf1N1B4_987 0.47 +0.7 1,118,091 - +1.1 1,118,091 - Pf1N1B4_987 0.47 -0.5 1,118,091 - Pf1N1B4_987 0.47 -0.6 1,118,091 - +0.5 1,118,091 - Pf1N1B4_987 0.47 +0.6 1,118,091 - Pf1N1B4_987 0.47 -1.4 1,118,108 + Pf1N1B4_987 0.48 -0.5 1,118,108 + Pf1N1B4_987 0.48 +0.6 1,118,109 - Pf1N1B4_987 0.48 -0.7 1,118,209 - Pf1N1B4_987 0.54 -1.3 1,118,214 + Pf1N1B4_987 0.54 +0.5 1,118,250 + Pf1N1B4_987 0.57 +0.4 1,118,250 + Pf1N1B4_987 0.57 +0.2 1,118,250 + Pf1N1B4_987 0.57 +1.1 1,118,250 + Pf1N1B4_987 0.57 -1.5 1,118,250 + Pf1N1B4_987 0.57 +2.3 1,118,251 - Pf1N1B4_987 0.57 +0.5 1,118,251 - Pf1N1B4_987 0.57 -0.6
Or see this region's nucleotide sequence