Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_982 and Pf1N1B4_983 are separated by 183 nucleotides Pf1N1B4_983 and Pf1N1B4_984 are separated by 35 nucleotides
Pf1N1B4_982 - GDP-mannose 6-dehydrogenase (EC 1.1.1.132), at 1,110,132 to 1,111,448
_982
Pf1N1B4_983 - Alginate biosynthesis protein Alg8, at 1,111,632 to 1,113,113
_983
Pf1N1B4_984 - Alginate biosynthesis protein Alg44, at 1,113,149 to 1,114,318
_984
Position (kb)
1111
1112
1113
1114 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1110.634 kb on - strand, within Pf1N1B4_982 at 1110.703 kb on - strand at 1110.703 kb on - strand, within Pf1N1B4_982 at 1110.703 kb on - strand, within Pf1N1B4_982 at 1110.762 kb on + strand, within Pf1N1B4_982 at 1110.762 kb on + strand, within Pf1N1B4_982 at 1110.763 kb on - strand, within Pf1N1B4_982 at 1110.763 kb on - strand, within Pf1N1B4_982 at 1110.763 kb on - strand, within Pf1N1B4_982 at 1110.763 kb on - strand, within Pf1N1B4_982 at 1110.763 kb on - strand, within Pf1N1B4_982 at 1110.763 kb on - strand, within Pf1N1B4_982 at 1110.763 kb on - strand at 1110.763 kb on - strand, within Pf1N1B4_982 at 1110.763 kb on - strand, within Pf1N1B4_982 at 1110.763 kb on - strand, within Pf1N1B4_982 at 1110.897 kb on + strand at 1110.898 kb on - strand, within Pf1N1B4_982 at 1110.900 kb on + strand, within Pf1N1B4_982 at 1110.900 kb on + strand, within Pf1N1B4_982 at 1110.900 kb on + strand, within Pf1N1B4_982 at 1110.900 kb on + strand at 1110.900 kb on + strand, within Pf1N1B4_982 at 1110.901 kb on - strand, within Pf1N1B4_982 at 1110.901 kb on - strand, within Pf1N1B4_982 at 1110.901 kb on - strand, within Pf1N1B4_982 at 1110.901 kb on - strand, within Pf1N1B4_982 at 1110.901 kb on - strand, within Pf1N1B4_982 at 1110.901 kb on - strand, within Pf1N1B4_982 at 1110.901 kb on - strand, within Pf1N1B4_982 at 1110.901 kb on - strand, within Pf1N1B4_982 at 1110.963 kb on + strand, within Pf1N1B4_982 at 1111.186 kb on - strand, within Pf1N1B4_982 at 1111.186 kb on - strand, within Pf1N1B4_982 at 1111.186 kb on - strand, within Pf1N1B4_982 at 1111.203 kb on + strand, within Pf1N1B4_982 at 1111.204 kb on - strand, within Pf1N1B4_982 at 1111.233 kb on + strand, within Pf1N1B4_982 at 1111.234 kb on - strand, within Pf1N1B4_982 at 1111.234 kb on - strand, within Pf1N1B4_982 at 1111.234 kb on - strand, within Pf1N1B4_982 at 1111.234 kb on - strand, within Pf1N1B4_982 at 1111.234 kb on - strand, within Pf1N1B4_982 at 1111.234 kb on - strand, within Pf1N1B4_982 at 1111.463 kb on - strand at 1111.508 kb on - strand at 1111.538 kb on - strand at 1111.567 kb on + strand at 1111.567 kb on + strand at 1111.567 kb on + strand at 1111.568 kb on - strand at 1111.631 kb on + strand at 1111.680 kb on + strand at 1111.680 kb on + strand at 1111.680 kb on + strand at 1111.680 kb on + strand at 1111.681 kb on - strand at 1111.685 kb on - strand at 1111.691 kb on - strand at 1111.718 kb on + strand at 1111.718 kb on + strand at 1111.719 kb on - strand at 1111.751 kb on + strand at 1111.751 kb on + strand at 1111.752 kb on - strand at 1111.752 kb on - strand at 1111.752 kb on - strand at 1111.752 kb on - strand at 1111.752 kb on - strand at 1111.752 kb on - strand at 1111.752 kb on - strand at 1111.752 kb on - strand at 1111.830 kb on + strand, within Pf1N1B4_983 at 1111.830 kb on + strand at 1111.830 kb on + strand, within Pf1N1B4_983 at 1111.831 kb on - strand, within Pf1N1B4_983 at 1111.897 kb on + strand, within Pf1N1B4_983 at 1111.897 kb on + strand, within Pf1N1B4_983 at 1111.897 kb on + strand, within Pf1N1B4_983 at 1111.897 kb on + strand, within Pf1N1B4_983 at 1111.898 kb on - strand, within Pf1N1B4_983 at 1111.898 kb on - strand, within Pf1N1B4_983 at 1111.898 kb on - strand, within Pf1N1B4_983 at 1111.898 kb on - strand, within Pf1N1B4_983 at 1111.898 kb on - strand, within Pf1N1B4_983 at 1111.951 kb on - strand, within Pf1N1B4_983 at 1112.304 kb on + strand, within Pf1N1B4_983 at 1112.304 kb on + strand, within Pf1N1B4_983 at 1112.562 kb on + strand at 1112.562 kb on + strand, within Pf1N1B4_983 at 1112.562 kb on + strand, within Pf1N1B4_983 at 1112.563 kb on - strand, within Pf1N1B4_983 at 1112.563 kb on - strand, within Pf1N1B4_983 at 1112.563 kb on - strand, within Pf1N1B4_983 at 1112.563 kb on - strand, within Pf1N1B4_983 at 1112.563 kb on - strand, within Pf1N1B4_983 at 1112.669 kb on + strand, within Pf1N1B4_983 at 1112.743 kb on + strand, within Pf1N1B4_983 at 1112.823 kb on + strand, within Pf1N1B4_983 at 1112.858 kb on + strand, within Pf1N1B4_983 at 1112.858 kb on + strand, within Pf1N1B4_983 at 1112.859 kb on - strand, within Pf1N1B4_983 at 1112.916 kb on + strand, within Pf1N1B4_983 at 1112.916 kb on + strand, within Pf1N1B4_983 at 1112.917 kb on - strand, within Pf1N1B4_983 at 1112.920 kb on - strand, within Pf1N1B4_983 at 1112.920 kb on - strand, within Pf1N1B4_983 at 1112.920 kb on - strand, within Pf1N1B4_983 at 1112.920 kb on - strand, within Pf1N1B4_983 at 1112.920 kb on - strand, within Pf1N1B4_983 at 1112.952 kb on + strand, within Pf1N1B4_983 at 1112.952 kb on + strand at 1112.952 kb on + strand at 1112.953 kb on - strand, within Pf1N1B4_983 at 1112.953 kb on - strand, within Pf1N1B4_983 at 1113.131 kb on - strand at 1113.131 kb on - strand at 1113.148 kb on + strand at 1113.148 kb on + strand at 1113.174 kb on + strand at 1113.174 kb on + strand at 1113.174 kb on + strand at 1113.175 kb on - strand at 1113.175 kb on - strand at 1113.526 kb on - strand, within Pf1N1B4_984 at 1113.643 kb on + strand, within Pf1N1B4_984 at 1113.693 kb on - strand, within Pf1N1B4_984 at 1113.701 kb on + strand, within Pf1N1B4_984 at 1113.701 kb on + strand, within Pf1N1B4_984 at 1113.701 kb on + strand, within Pf1N1B4_984 at 1113.701 kb on + strand, within Pf1N1B4_984 at 1113.701 kb on + strand, within Pf1N1B4_984 at 1113.701 kb on + strand, within Pf1N1B4_984 at 1113.702 kb on - strand, within Pf1N1B4_984 at 1113.856 kb on - strand, within Pf1N1B4_984 at 1113.856 kb on - strand, within Pf1N1B4_984 at 1113.856 kb on - strand at 1113.989 kb on + strand, within Pf1N1B4_984
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 1,110,634 - Pf1N1B4_982 0.38 +0.1 1,110,703 - +0.5 1,110,703 - Pf1N1B4_982 0.43 -1.5 1,110,703 - Pf1N1B4_982 0.43 +1.2 1,110,762 + Pf1N1B4_982 0.48 +0.1 1,110,762 + Pf1N1B4_982 0.48 +1.1 1,110,763 - Pf1N1B4_982 0.48 -2.0 1,110,763 - Pf1N1B4_982 0.48 -0.9 1,110,763 - Pf1N1B4_982 0.48 +0.1 1,110,763 - Pf1N1B4_982 0.48 +0.4 1,110,763 - Pf1N1B4_982 0.48 +0.1 1,110,763 - Pf1N1B4_982 0.48 +1.5 1,110,763 - +1.1 1,110,763 - Pf1N1B4_982 0.48 +0.9 1,110,763 - Pf1N1B4_982 0.48 +0.5 1,110,763 - Pf1N1B4_982 0.48 -0.5 1,110,897 + -0.5 1,110,898 - Pf1N1B4_982 0.58 +0.1 1,110,900 + Pf1N1B4_982 0.58 +0.3 1,110,900 + Pf1N1B4_982 0.58 -0.3 1,110,900 + Pf1N1B4_982 0.58 -1.7 1,110,900 + +1.1 1,110,900 + Pf1N1B4_982 0.58 -1.2 1,110,901 - Pf1N1B4_982 0.58 -0.7 1,110,901 - Pf1N1B4_982 0.58 -0.9 1,110,901 - Pf1N1B4_982 0.58 -0.3 1,110,901 - Pf1N1B4_982 0.58 -1.9 1,110,901 - Pf1N1B4_982 0.58 +0.1 1,110,901 - Pf1N1B4_982 0.58 -0.5 1,110,901 - Pf1N1B4_982 0.58 -0.5 1,110,901 - Pf1N1B4_982 0.58 +1.8 1,110,963 + Pf1N1B4_982 0.63 -0.8 1,111,186 - Pf1N1B4_982 0.80 -0.2 1,111,186 - Pf1N1B4_982 0.80 -1.5 1,111,186 - Pf1N1B4_982 0.80 -0.5 1,111,203 + Pf1N1B4_982 0.81 +0.1 1,111,204 - Pf1N1B4_982 0.81 +1.0 1,111,233 + Pf1N1B4_982 0.84 -0.9 1,111,234 - Pf1N1B4_982 0.84 -0.2 1,111,234 - Pf1N1B4_982 0.84 +1.1 1,111,234 - Pf1N1B4_982 0.84 -2.7 1,111,234 - Pf1N1B4_982 0.84 +1.4 1,111,234 - Pf1N1B4_982 0.84 +0.8 1,111,234 - Pf1N1B4_982 0.84 -1.7 1,111,463 - +0.5 1,111,508 - +1.1 1,111,538 - -1.7 1,111,567 + -0.7 1,111,567 + -0.9 1,111,567 + -1.0 1,111,568 - -1.0 1,111,631 + -0.5 1,111,680 + +0.3 1,111,680 + -0.5 1,111,680 + +0.1 1,111,680 + +0.7 1,111,681 - +0.7 1,111,685 - +0.4 1,111,691 - +0.5 1,111,718 + +0.4 1,111,718 + +3.2 1,111,719 - +1.1 1,111,751 + +0.4 1,111,751 + +0.4 1,111,752 - -1.1 1,111,752 - +0.3 1,111,752 - +0.4 1,111,752 - +0.5 1,111,752 - -0.5 1,111,752 - -0.0 1,111,752 - +1.1 1,111,752 - +0.2 1,111,830 + Pf1N1B4_983 0.13 +0.2 1,111,830 + -1.5 1,111,830 + Pf1N1B4_983 0.13 +0.7 1,111,831 - Pf1N1B4_983 0.13 -1.0 1,111,897 + Pf1N1B4_983 0.18 -0.1 1,111,897 + Pf1N1B4_983 0.18 -0.5 1,111,897 + Pf1N1B4_983 0.18 -0.9 1,111,897 + Pf1N1B4_983 0.18 -0.5 1,111,898 - Pf1N1B4_983 0.18 -1.2 1,111,898 - Pf1N1B4_983 0.18 +0.0 1,111,898 - Pf1N1B4_983 0.18 +0.8 1,111,898 - Pf1N1B4_983 0.18 +0.3 1,111,898 - Pf1N1B4_983 0.18 -1.7 1,111,951 - Pf1N1B4_983 0.22 +0.5 1,112,304 + Pf1N1B4_983 0.45 +1.4 1,112,304 + Pf1N1B4_983 0.45 -0.2 1,112,562 + +0.8 1,112,562 + Pf1N1B4_983 0.63 +0.9 1,112,562 + Pf1N1B4_983 0.63 -0.8 1,112,563 - Pf1N1B4_983 0.63 -0.5 1,112,563 - Pf1N1B4_983 0.63 -0.0 1,112,563 - Pf1N1B4_983 0.63 -0.1 1,112,563 - Pf1N1B4_983 0.63 -0.2 1,112,563 - Pf1N1B4_983 0.63 -0.3 1,112,669 + Pf1N1B4_983 0.70 -0.5 1,112,743 + Pf1N1B4_983 0.75 -1.9 1,112,823 + Pf1N1B4_983 0.80 -0.3 1,112,858 + Pf1N1B4_983 0.83 -0.9 1,112,858 + Pf1N1B4_983 0.83 -1.1 1,112,859 - Pf1N1B4_983 0.83 +0.9 1,112,916 + Pf1N1B4_983 0.87 +0.1 1,112,916 + Pf1N1B4_983 0.87 -1.2 1,112,917 - Pf1N1B4_983 0.87 +1.3 1,112,920 - Pf1N1B4_983 0.87 +0.5 1,112,920 - Pf1N1B4_983 0.87 -0.0 1,112,920 - Pf1N1B4_983 0.87 +0.5 1,112,920 - Pf1N1B4_983 0.87 -1.2 1,112,920 - Pf1N1B4_983 0.87 -0.2 1,112,952 + Pf1N1B4_983 0.89 -1.2 1,112,952 + +2.4 1,112,952 + -1.7 1,112,953 - Pf1N1B4_983 0.89 +0.3 1,112,953 - Pf1N1B4_983 0.89 +0.4 1,113,131 - -0.1 1,113,131 - +0.4 1,113,148 + +0.1 1,113,148 + -0.3 1,113,174 + -0.2 1,113,174 + +0.3 1,113,174 + +2.3 1,113,175 - -2.0 1,113,175 - +1.5 1,113,526 - Pf1N1B4_984 0.32 -1.9 1,113,643 + Pf1N1B4_984 0.42 +1.4 1,113,693 - Pf1N1B4_984 0.46 +0.4 1,113,701 + Pf1N1B4_984 0.47 -0.9 1,113,701 + Pf1N1B4_984 0.47 -1.5 1,113,701 + Pf1N1B4_984 0.47 +1.5 1,113,701 + Pf1N1B4_984 0.47 +0.3 1,113,701 + Pf1N1B4_984 0.47 +1.4 1,113,701 + Pf1N1B4_984 0.47 +2.1 1,113,702 - Pf1N1B4_984 0.47 +2.3 1,113,856 - Pf1N1B4_984 0.60 -0.2 1,113,856 - Pf1N1B4_984 0.60 -2.8 1,113,856 - -0.9 1,113,989 + Pf1N1B4_984 0.72 -0.5
Or see this region's nucleotide sequence