Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_804 and Pf1N1B4_805 are separated by 34 nucleotides Pf1N1B4_805 and Pf1N1B4_806 are separated by 193 nucleotides
Pf1N1B4_804 - hypothetical protein, at 919,369 to 921,210
_804
Pf1N1B4_805 - hypothetical protein, at 921,245 to 922,516
_805
Pf1N1B4_806 - hypothetical protein, at 922,710 to 924,479
_806
Position (kb)
921
922
923 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 920.284 kb on + strand, within Pf1N1B4_804 at 920.284 kb on + strand, within Pf1N1B4_804 at 920.284 kb on + strand, within Pf1N1B4_804 at 920.284 kb on + strand, within Pf1N1B4_804 at 920.285 kb on - strand, within Pf1N1B4_804 at 920.285 kb on - strand, within Pf1N1B4_804 at 920.285 kb on - strand, within Pf1N1B4_804 at 920.285 kb on - strand, within Pf1N1B4_804 at 920.596 kb on + strand, within Pf1N1B4_804 at 920.642 kb on - strand, within Pf1N1B4_804 at 920.833 kb on + strand, within Pf1N1B4_804 at 920.833 kb on + strand, within Pf1N1B4_804 at 920.834 kb on - strand, within Pf1N1B4_804 at 920.834 kb on - strand, within Pf1N1B4_804 at 920.918 kb on + strand, within Pf1N1B4_804 at 920.918 kb on + strand, within Pf1N1B4_804 at 920.918 kb on + strand, within Pf1N1B4_804 at 920.918 kb on + strand, within Pf1N1B4_804 at 920.919 kb on - strand, within Pf1N1B4_804 at 920.919 kb on - strand, within Pf1N1B4_804 at 920.919 kb on - strand at 920.919 kb on - strand, within Pf1N1B4_804 at 920.919 kb on - strand at 920.919 kb on - strand, within Pf1N1B4_804 at 921.416 kb on + strand, within Pf1N1B4_805 at 921.416 kb on + strand, within Pf1N1B4_805 at 921.416 kb on + strand, within Pf1N1B4_805 at 921.416 kb on + strand, within Pf1N1B4_805 at 921.416 kb on + strand, within Pf1N1B4_805 at 921.417 kb on - strand, within Pf1N1B4_805 at 921.417 kb on - strand, within Pf1N1B4_805 at 921.476 kb on + strand, within Pf1N1B4_805 at 921.476 kb on + strand, within Pf1N1B4_805 at 921.476 kb on + strand, within Pf1N1B4_805 at 921.476 kb on + strand, within Pf1N1B4_805 at 921.477 kb on - strand, within Pf1N1B4_805 at 921.645 kb on - strand, within Pf1N1B4_805 at 921.824 kb on + strand, within Pf1N1B4_805 at 921.824 kb on + strand, within Pf1N1B4_805 at 921.824 kb on + strand, within Pf1N1B4_805 at 921.824 kb on + strand, within Pf1N1B4_805 at 921.825 kb on - strand, within Pf1N1B4_805 at 921.838 kb on + strand, within Pf1N1B4_805 at 921.839 kb on - strand, within Pf1N1B4_805 at 922.015 kb on + strand, within Pf1N1B4_805 at 922.016 kb on - strand, within Pf1N1B4_805 at 922.016 kb on - strand, within Pf1N1B4_805 at 922.127 kb on - strand, within Pf1N1B4_805 at 922.127 kb on - strand, within Pf1N1B4_805 at 922.149 kb on + strand, within Pf1N1B4_805 at 922.149 kb on + strand, within Pf1N1B4_805 at 922.149 kb on + strand, within Pf1N1B4_805 at 922.149 kb on + strand, within Pf1N1B4_805 at 922.149 kb on + strand, within Pf1N1B4_805 at 922.150 kb on - strand, within Pf1N1B4_805 at 922.271 kb on + strand, within Pf1N1B4_805 at 922.271 kb on + strand, within Pf1N1B4_805 at 922.271 kb on + strand, within Pf1N1B4_805 at 922.272 kb on - strand, within Pf1N1B4_805 at 922.272 kb on - strand, within Pf1N1B4_805 at 922.272 kb on - strand, within Pf1N1B4_805 at 922.320 kb on - strand, within Pf1N1B4_805 at 922.562 kb on - strand at 922.846 kb on + strand at 923.322 kb on - strand, within Pf1N1B4_806
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 920,284 + Pf1N1B4_804 0.50 -1.5 920,284 + Pf1N1B4_804 0.50 -0.9 920,284 + Pf1N1B4_804 0.50 -1.1 920,284 + Pf1N1B4_804 0.50 -0.7 920,285 - Pf1N1B4_804 0.50 +1.2 920,285 - Pf1N1B4_804 0.50 -1.9 920,285 - Pf1N1B4_804 0.50 -0.7 920,285 - Pf1N1B4_804 0.50 +0.1 920,596 + Pf1N1B4_804 0.67 -0.1 920,642 - Pf1N1B4_804 0.69 -1.8 920,833 + Pf1N1B4_804 0.79 -0.4 920,833 + Pf1N1B4_804 0.79 +0.5 920,834 - Pf1N1B4_804 0.80 +0.5 920,834 - Pf1N1B4_804 0.80 +1.7 920,918 + Pf1N1B4_804 0.84 -0.3 920,918 + Pf1N1B4_804 0.84 -0.5 920,918 + Pf1N1B4_804 0.84 -1.2 920,918 + Pf1N1B4_804 0.84 -0.9 920,919 - Pf1N1B4_804 0.84 +1.8 920,919 - Pf1N1B4_804 0.84 -1.2 920,919 - +2.1 920,919 - Pf1N1B4_804 0.84 -0.1 920,919 - -1.2 920,919 - Pf1N1B4_804 0.84 +0.8 921,416 + Pf1N1B4_805 0.13 -0.5 921,416 + Pf1N1B4_805 0.13 -1.7 921,416 + Pf1N1B4_805 0.13 +0.2 921,416 + Pf1N1B4_805 0.13 -0.7 921,416 + Pf1N1B4_805 0.13 -0.2 921,417 - Pf1N1B4_805 0.14 +0.7 921,417 - Pf1N1B4_805 0.14 -1.0 921,476 + Pf1N1B4_805 0.18 -0.9 921,476 + Pf1N1B4_805 0.18 +1.1 921,476 + Pf1N1B4_805 0.18 +1.1 921,476 + Pf1N1B4_805 0.18 -1.1 921,477 - Pf1N1B4_805 0.18 +0.7 921,645 - Pf1N1B4_805 0.31 -1.2 921,824 + Pf1N1B4_805 0.46 -0.2 921,824 + Pf1N1B4_805 0.46 +0.5 921,824 + Pf1N1B4_805 0.46 -1.5 921,824 + Pf1N1B4_805 0.46 +0.1 921,825 - Pf1N1B4_805 0.46 -0.5 921,838 + Pf1N1B4_805 0.47 -0.2 921,839 - Pf1N1B4_805 0.47 -0.3 922,015 + Pf1N1B4_805 0.61 +0.5 922,016 - Pf1N1B4_805 0.61 -0.9 922,016 - Pf1N1B4_805 0.61 +0.9 922,127 - Pf1N1B4_805 0.69 +0.3 922,127 - Pf1N1B4_805 0.69 +0.1 922,149 + Pf1N1B4_805 0.71 -0.6 922,149 + Pf1N1B4_805 0.71 -0.4 922,149 + Pf1N1B4_805 0.71 -1.7 922,149 + Pf1N1B4_805 0.71 +0.1 922,149 + Pf1N1B4_805 0.71 +1.0 922,150 - Pf1N1B4_805 0.71 -1.2 922,271 + Pf1N1B4_805 0.81 +0.1 922,271 + Pf1N1B4_805 0.81 -1.2 922,271 + Pf1N1B4_805 0.81 -0.5 922,272 - Pf1N1B4_805 0.81 -0.1 922,272 - Pf1N1B4_805 0.81 -0.7 922,272 - Pf1N1B4_805 0.81 -0.3 922,320 - Pf1N1B4_805 0.85 -0.5 922,562 - -0.0 922,846 + -0.9 923,322 - Pf1N1B4_806 0.35 -1.7
Or see this region's nucleotide sequence