Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_763

Experiment: Spectinomycin 0.05 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_762 and Pf1N1B4_763 overlap by 4 nucleotidesPf1N1B4_763 and Pf1N1B4_764 are separated by 115 nucleotides Pf1N1B4_762 - Ornithine carbamoyltransferase (EC 2.1.3.3), at 866,173 to 867,096 _762 Pf1N1B4_763 - Ferric iron ABC transporter, ATP-binding protein, at 867,093 to 868,202 _763 Pf1N1B4_764 - Uncharacterized isomerase yddE, PhzC-PhzF family, at 868,318 to 869,100 _764 Position (kb) 867 868 869Strain fitness (log2 ratio) -2 -1 0 1 2at 866.098 kb on + strandat 866.128 kb on - strandat 866.143 kb on + strandat 866.674 kb on + strand, within Pf1N1B4_762at 866.674 kb on + strand, within Pf1N1B4_762at 866.674 kb on + strand, within Pf1N1B4_762at 866.674 kb on + strand, within Pf1N1B4_762at 866.674 kb on + strand, within Pf1N1B4_762at 866.674 kb on + strand, within Pf1N1B4_762at 866.674 kb on + strand, within Pf1N1B4_762at 866.674 kb on + strand, within Pf1N1B4_762at 866.675 kb on - strand, within Pf1N1B4_762at 866.675 kb on - strand, within Pf1N1B4_762at 866.676 kb on + strand, within Pf1N1B4_762at 866.676 kb on + strand, within Pf1N1B4_762at 866.676 kb on + strand, within Pf1N1B4_762at 866.677 kb on - strand, within Pf1N1B4_762at 866.678 kb on + strand, within Pf1N1B4_762at 866.678 kb on + strand, within Pf1N1B4_762at 866.678 kb on + strand, within Pf1N1B4_762at 866.678 kb on + strand, within Pf1N1B4_762at 866.678 kb on + strandat 866.678 kb on + strand, within Pf1N1B4_762at 866.678 kb on + strand, within Pf1N1B4_762at 866.678 kb on + strand, within Pf1N1B4_762at 866.678 kb on + strand, within Pf1N1B4_762at 866.678 kb on + strand, within Pf1N1B4_762at 866.678 kb on + strand, within Pf1N1B4_762at 866.679 kb on - strand, within Pf1N1B4_762at 866.679 kb on - strand, within Pf1N1B4_762at 866.679 kb on - strand, within Pf1N1B4_762at 866.679 kb on - strand, within Pf1N1B4_762at 866.679 kb on - strand, within Pf1N1B4_762at 866.679 kb on - strand, within Pf1N1B4_762at 866.679 kb on - strand, within Pf1N1B4_762at 866.679 kb on - strand, within Pf1N1B4_762at 866.679 kb on - strand, within Pf1N1B4_762at 866.679 kb on - strand, within Pf1N1B4_762at 866.679 kb on - strand, within Pf1N1B4_762at 866.714 kb on + strand, within Pf1N1B4_762at 867.628 kb on + strand, within Pf1N1B4_763at 867.628 kb on + strand, within Pf1N1B4_763at 867.629 kb on - strandat 867.629 kb on - strand, within Pf1N1B4_763at 867.629 kb on - strand, within Pf1N1B4_763at 867.757 kb on - strand, within Pf1N1B4_763at 867.765 kb on + strand, within Pf1N1B4_763at 867.814 kb on - strand, within Pf1N1B4_763at 867.832 kb on + strand, within Pf1N1B4_763at 867.833 kb on - strand, within Pf1N1B4_763at 867.872 kb on - strand, within Pf1N1B4_763at 867.872 kb on - strand, within Pf1N1B4_763at 867.888 kb on + strand, within Pf1N1B4_763at 867.888 kb on + strand, within Pf1N1B4_763at 867.889 kb on - strand, within Pf1N1B4_763at 867.889 kb on - strand, within Pf1N1B4_763at 867.889 kb on - strand, within Pf1N1B4_763at 868.090 kb on + strand, within Pf1N1B4_763at 868.249 kb on - strandat 868.274 kb on + strandat 868.275 kb on - strandat 868.275 kb on - strandat 868.275 kb on - strandat 868.330 kb on + strandat 868.331 kb on - strandat 868.331 kb on - strandat 868.331 kb on - strandat 868.331 kb on - strandat 868.427 kb on + strand, within Pf1N1B4_764at 868.428 kb on - strand, within Pf1N1B4_764at 868.654 kb on + strand, within Pf1N1B4_764at 868.655 kb on - strand, within Pf1N1B4_764at 868.844 kb on + strand, within Pf1N1B4_764at 868.844 kb on + strand, within Pf1N1B4_764at 868.845 kb on - strand, within Pf1N1B4_764at 868.845 kb on - strand, within Pf1N1B4_764at 868.845 kb on - strand, within Pf1N1B4_764at 868.845 kb on - strand, within Pf1N1B4_764at 868.845 kb on - strand, within Pf1N1B4_764at 868.845 kb on - strand, within Pf1N1B4_764at 868.862 kb on + strand, within Pf1N1B4_764at 868.863 kb on - strand, within Pf1N1B4_764at 868.863 kb on - strand, within Pf1N1B4_764at 868.863 kb on - strand, within Pf1N1B4_764at 868.914 kb on + strand, within Pf1N1B4_764at 869.092 kb on + strandat 869.093 kb on - strandat 869.093 kb on - strandat 869.093 kb on - strandat 869.183 kb on + strandat 869.184 kb on - strandat 869.184 kb on - strandat 869.184 kb on - strandat 869.184 kb on - strandat 869.184 kb on - strandat 869.184 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml
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866,098 + +0.0
866,128 - -0.3
866,143 + +1.6
866,674 + Pf1N1B4_762 0.54 -1.3
866,674 + Pf1N1B4_762 0.54 +0.6
866,674 + Pf1N1B4_762 0.54 +0.8
866,674 + Pf1N1B4_762 0.54 -0.7
866,674 + Pf1N1B4_762 0.54 +1.3
866,674 + Pf1N1B4_762 0.54 +0.2
866,674 + Pf1N1B4_762 0.54 +0.2
866,674 + Pf1N1B4_762 0.54 +1.0
866,675 - Pf1N1B4_762 0.54 -1.5
866,675 - Pf1N1B4_762 0.54 -1.8
866,676 + Pf1N1B4_762 0.54 -0.8
866,676 + Pf1N1B4_762 0.54 -1.0
866,676 + Pf1N1B4_762 0.54 -1.0
866,677 - Pf1N1B4_762 0.55 +1.4
866,678 + Pf1N1B4_762 0.55 -1.5
866,678 + Pf1N1B4_762 0.55 +0.0
866,678 + Pf1N1B4_762 0.55 +1.0
866,678 + Pf1N1B4_762 0.55 +0.7
866,678 + +1.0
866,678 + Pf1N1B4_762 0.55 -0.2
866,678 + Pf1N1B4_762 0.55 +0.0
866,678 + Pf1N1B4_762 0.55 -1.5
866,678 + Pf1N1B4_762 0.55 +0.6
866,678 + Pf1N1B4_762 0.55 -0.8
866,678 + Pf1N1B4_762 0.55 +1.5
866,679 - Pf1N1B4_762 0.55 +1.0
866,679 - Pf1N1B4_762 0.55 -0.9
866,679 - Pf1N1B4_762 0.55 -0.1
866,679 - Pf1N1B4_762 0.55 -2.1
866,679 - Pf1N1B4_762 0.55 +0.7
866,679 - Pf1N1B4_762 0.55 +0.2
866,679 - Pf1N1B4_762 0.55 +0.5
866,679 - Pf1N1B4_762 0.55 +0.0
866,679 - Pf1N1B4_762 0.55 -1.3
866,679 - Pf1N1B4_762 0.55 +0.6
866,679 - Pf1N1B4_762 0.55 +0.6
866,714 + Pf1N1B4_762 0.59 -1.0
867,628 + Pf1N1B4_763 0.48 -2.1
867,628 + Pf1N1B4_763 0.48 -1.0
867,629 - +0.0
867,629 - Pf1N1B4_763 0.48 -0.2
867,629 - Pf1N1B4_763 0.48 +1.5
867,757 - Pf1N1B4_763 0.60 +0.0
867,765 + Pf1N1B4_763 0.61 -0.5
867,814 - Pf1N1B4_763 0.65 -2.3
867,832 + Pf1N1B4_763 0.67 -1.3
867,833 - Pf1N1B4_763 0.67 -0.5
867,872 - Pf1N1B4_763 0.70 -2.7
867,872 - Pf1N1B4_763 0.70 -2.0
867,888 + Pf1N1B4_763 0.72 +0.0
867,888 + Pf1N1B4_763 0.72 -0.5
867,889 - Pf1N1B4_763 0.72 +0.8
867,889 - Pf1N1B4_763 0.72 +0.0
867,889 - Pf1N1B4_763 0.72 +0.3
868,090 + Pf1N1B4_763 0.90 -1.7
868,249 - -1.5
868,274 + +1.0
868,275 - -1.0
868,275 - +2.0
868,275 - -1.0
868,330 + -1.0
868,331 - -1.4
868,331 - -1.0
868,331 - +1.3
868,331 - -0.4
868,427 + Pf1N1B4_764 0.14 -0.5
868,428 - Pf1N1B4_764 0.14 +1.0
868,654 + Pf1N1B4_764 0.43 -0.8
868,655 - Pf1N1B4_764 0.43 +0.1
868,844 + Pf1N1B4_764 0.67 -0.3
868,844 + Pf1N1B4_764 0.67 +0.5
868,845 - Pf1N1B4_764 0.67 -1.0
868,845 - Pf1N1B4_764 0.67 -1.1
868,845 - Pf1N1B4_764 0.67 -2.0
868,845 - Pf1N1B4_764 0.67 +0.3
868,845 - Pf1N1B4_764 0.67 +1.0
868,845 - Pf1N1B4_764 0.67 -1.0
868,862 + Pf1N1B4_764 0.69 -1.3
868,863 - Pf1N1B4_764 0.70 +0.8
868,863 - Pf1N1B4_764 0.70 +1.0
868,863 - Pf1N1B4_764 0.70 -1.5
868,914 + Pf1N1B4_764 0.76 +0.0
869,092 + +0.4
869,093 - +0.1
869,093 - -0.5
869,093 - +2.0
869,183 + +1.0
869,184 - -0.3
869,184 - -0.1
869,184 - +0.5
869,184 - +1.6
869,184 - +0.5
869,184 - -0.5

Or see this region's nucleotide sequence