Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_708 and Pf1N1B4_709 are separated by 96 nucleotides Pf1N1B4_709 and Pf1N1B4_710 are separated by 6 nucleotides Pf1N1B4_710 and Pf1N1B4_711 are separated by 176 nucleotides Pf1N1B4_711 and Pf1N1B4_712 are separated by 113 nucleotides
Pf1N1B4_708 - Chromosome initiation inhibitor, at 808,026 to 808,925
_708
Pf1N1B4_709 - FIG00953860: hypothetical protein, at 809,022 to 809,423
_709
Pf1N1B4_710 - Transporter, LysE family, at 809,430 to 810,032
_710
Pf1N1B4_711 - Superoxide dismutase [Fe] (EC 1.15.1.1), at 810,209 to 810,805
_711
Pf1N1B4_712 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 810,919 to 811,287
_712
Position (kb) 809
810
811 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 808.549 kb on - strand, within Pf1N1B4_708 at 808.908 kb on + strand at 808.908 kb on + strand at 808.909 kb on - strand at 808.909 kb on - strand at 808.916 kb on - strand at 809.088 kb on + strand, within Pf1N1B4_709 at 809.088 kb on + strand, within Pf1N1B4_709 at 809.088 kb on + strand, within Pf1N1B4_709 at 809.088 kb on + strand, within Pf1N1B4_709 at 809.088 kb on + strand, within Pf1N1B4_709 at 809.089 kb on - strand, within Pf1N1B4_709 at 809.089 kb on - strand, within Pf1N1B4_709 at 809.089 kb on - strand, within Pf1N1B4_709 at 809.220 kb on + strand, within Pf1N1B4_709 at 809.221 kb on - strand, within Pf1N1B4_709 at 809.221 kb on - strand at 809.221 kb on - strand, within Pf1N1B4_709 at 809.221 kb on - strand, within Pf1N1B4_709 at 809.341 kb on - strand, within Pf1N1B4_709 at 809.341 kb on - strand at 809.393 kb on - strand at 809.421 kb on + strand at 809.429 kb on + strand at 809.429 kb on + strand at 809.430 kb on - strand at 809.430 kb on - strand at 809.430 kb on - strand at 809.442 kb on + strand at 809.442 kb on + strand at 809.442 kb on + strand at 809.442 kb on + strand at 809.442 kb on + strand at 809.442 kb on + strand at 809.669 kb on - strand, within Pf1N1B4_710 at 809.669 kb on - strand at 809.744 kb on - strand at 809.806 kb on - strand at 809.865 kb on + strand, within Pf1N1B4_710 at 809.865 kb on + strand, within Pf1N1B4_710 at 809.866 kb on - strand, within Pf1N1B4_710 at 809.866 kb on - strand, within Pf1N1B4_710 at 809.893 kb on + strand, within Pf1N1B4_710 at 809.893 kb on + strand, within Pf1N1B4_710 at 809.912 kb on - strand, within Pf1N1B4_710 at 810.017 kb on - strand at 810.036 kb on - strand at 810.059 kb on + strand at 810.102 kb on + strand at 810.102 kb on + strand at 810.102 kb on + strand at 810.102 kb on + strand at 810.102 kb on + strand at 810.103 kb on - strand at 810.103 kb on - strand at 810.103 kb on - strand at 810.103 kb on - strand at 810.130 kb on + strand at 810.130 kb on + strand at 810.130 kb on + strand at 810.196 kb on + strand at 810.196 kb on + strand at 810.196 kb on + strand at 810.196 kb on + strand at 810.803 kb on + strand at 810.803 kb on + strand at 810.803 kb on + strand at 810.803 kb on + strand at 810.804 kb on - strand at 810.920 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 808,549 - Pf1N1B4_708 0.58 -2.0 808,908 + -1.8 808,908 + -2.0 808,909 - -1.2 808,909 - -0.6 808,916 - -0.0 809,088 + Pf1N1B4_709 0.16 -0.0 809,088 + Pf1N1B4_709 0.16 +0.0 809,088 + Pf1N1B4_709 0.16 -1.0 809,088 + Pf1N1B4_709 0.16 +0.2 809,088 + Pf1N1B4_709 0.16 +1.0 809,089 - Pf1N1B4_709 0.17 +2.0 809,089 - Pf1N1B4_709 0.17 -0.5 809,089 - Pf1N1B4_709 0.17 -0.9 809,220 + Pf1N1B4_709 0.49 -0.4 809,221 - Pf1N1B4_709 0.50 -0.0 809,221 - +1.0 809,221 - Pf1N1B4_709 0.50 -3.9 809,221 - Pf1N1B4_709 0.50 -0.8 809,341 - Pf1N1B4_709 0.79 -1.8 809,341 - -0.6 809,393 - -0.1 809,421 + -0.2 809,429 + +0.4 809,429 + -0.8 809,430 - -1.2 809,430 - +0.2 809,430 - -2.0 809,442 + -1.1 809,442 + -1.0 809,442 + -1.4 809,442 + +1.0 809,442 + -0.1 809,442 + -1.8 809,669 - Pf1N1B4_710 0.40 -1.3 809,669 - -0.0 809,744 - +1.0 809,806 - -0.6 809,865 + Pf1N1B4_710 0.72 -0.6 809,865 + Pf1N1B4_710 0.72 -1.2 809,866 - Pf1N1B4_710 0.72 -0.8 809,866 - Pf1N1B4_710 0.72 -0.0 809,893 + Pf1N1B4_710 0.77 -1.6 809,893 + Pf1N1B4_710 0.77 -2.3 809,912 - Pf1N1B4_710 0.80 -0.6 810,017 - -2.3 810,036 - -2.0 810,059 + -0.6 810,102 + -0.0 810,102 + -0.0 810,102 + +1.0 810,102 + -0.8 810,102 + +0.5 810,103 - +1.0 810,103 - -1.0 810,103 - -0.6 810,103 - -1.0 810,130 + +1.0 810,130 + +0.6 810,130 + -1.6 810,196 + -0.6 810,196 + +0.5 810,196 + +0.4 810,196 + -0.2 810,803 + -0.6 810,803 + -1.6 810,803 + -2.3 810,803 + -0.5 810,804 - +1.0 810,920 + +1.4
Or see this region's nucleotide sequence