Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_696 and Pf1N1B4_697 are separated by 159 nucleotides Pf1N1B4_697 and Pf1N1B4_698 are separated by 115 nucleotides Pf1N1B4_698 and Pf1N1B4_699 are separated by 155 nucleotides
Pf1N1B4_696 - Acetolactate synthase, large subunit (EC 2.2.1.6), at 794,978 to 796,615
_696
Pf1N1B4_697 - Glycine cleavage system transcriptional activator, at 796,775 to 797,656
_697
Pf1N1B4_698 - Rrf2-linked NADH-flavin reductase, at 797,772 to 798,386
_698
Pf1N1B4_699 - Metallo-beta-lactamase superfamily protein PA0057, at 798,542 to 799,423
_699
Position (kb) 796
797
798 Strain fitness (log2 ratio) -2
-1
0
1
2 at 796.356 kb on + strand, within Pf1N1B4_696 at 796.356 kb on + strand, within Pf1N1B4_696 at 796.583 kb on + strand at 796.595 kb on + strand at 796.595 kb on + strand at 796.595 kb on + strand at 796.595 kb on + strand at 796.595 kb on + strand at 796.595 kb on + strand at 796.596 kb on - strand at 796.596 kb on - strand at 796.596 kb on - strand at 796.663 kb on + strand at 796.663 kb on + strand at 796.664 kb on - strand at 796.664 kb on - strand at 796.664 kb on - strand at 796.729 kb on + strand at 796.739 kb on + strand at 796.740 kb on - strand at 796.740 kb on - strand at 796.742 kb on + strand at 796.743 kb on - strand at 796.743 kb on - strand at 796.761 kb on + strand at 796.762 kb on - strand at 796.762 kb on - strand at 796.762 kb on - strand at 796.762 kb on - strand at 796.919 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.205 kb on - strand, within Pf1N1B4_697 at 797.567 kb on + strand, within Pf1N1B4_697 at 797.570 kb on + strand at 797.787 kb on - strand at 797.804 kb on - strand at 797.804 kb on - strand at 797.804 kb on - strand at 797.855 kb on + strand, within Pf1N1B4_698 at 797.856 kb on - strand, within Pf1N1B4_698 at 797.856 kb on - strand, within Pf1N1B4_698 at 797.912 kb on + strand, within Pf1N1B4_698 at 797.990 kb on + strand, within Pf1N1B4_698 at 797.991 kb on - strand, within Pf1N1B4_698 at 797.991 kb on - strand, within Pf1N1B4_698 at 798.028 kb on + strand, within Pf1N1B4_698 at 798.028 kb on + strand, within Pf1N1B4_698 at 798.028 kb on + strand, within Pf1N1B4_698 at 798.028 kb on + strand, within Pf1N1B4_698 at 798.029 kb on - strand, within Pf1N1B4_698 at 798.029 kb on - strand, within Pf1N1B4_698 at 798.029 kb on - strand, within Pf1N1B4_698
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 796,356 + Pf1N1B4_696 0.84 +1.6 796,356 + Pf1N1B4_696 0.84 +0.4 796,583 + -2.5 796,595 + -1.0 796,595 + -0.0 796,595 + +1.0 796,595 + -1.0 796,595 + -1.2 796,595 + -1.4 796,596 - +0.7 796,596 - -0.0 796,596 - +1.6 796,663 + +2.5 796,663 + -0.9 796,664 - -0.4 796,664 - +0.3 796,664 - +1.5 796,729 + -0.8 796,739 + -1.6 796,740 - -0.0 796,740 - +1.4 796,742 + +0.4 796,743 - +1.4 796,743 - +1.0 796,761 + +0.4 796,762 - -0.0 796,762 - +1.6 796,762 - -1.5 796,762 - -1.6 796,919 + Pf1N1B4_697 0.16 +0.1 797,204 + Pf1N1B4_697 0.49 -1.0 797,204 + Pf1N1B4_697 0.49 -0.6 797,204 + Pf1N1B4_697 0.49 -0.9 797,204 + Pf1N1B4_697 0.49 -1.0 797,204 + Pf1N1B4_697 0.49 -0.0 797,204 + Pf1N1B4_697 0.49 -0.1 797,204 + Pf1N1B4_697 0.49 +0.6 797,204 + Pf1N1B4_697 0.49 -2.0 797,204 + Pf1N1B4_697 0.49 -2.0 797,204 + Pf1N1B4_697 0.49 +1.0 797,205 - Pf1N1B4_697 0.49 -0.8 797,567 + Pf1N1B4_697 0.90 -0.6 797,570 + -0.8 797,787 - -1.4 797,804 - -0.0 797,804 - +0.3 797,804 - +1.0 797,855 + Pf1N1B4_698 0.13 -0.9 797,856 - Pf1N1B4_698 0.14 +0.6 797,856 - Pf1N1B4_698 0.14 -1.8 797,912 + Pf1N1B4_698 0.23 -0.0 797,990 + Pf1N1B4_698 0.35 +0.6 797,991 - Pf1N1B4_698 0.36 +0.8 797,991 - Pf1N1B4_698 0.36 -0.4 798,028 + Pf1N1B4_698 0.42 -0.6 798,028 + Pf1N1B4_698 0.42 +0.2 798,028 + Pf1N1B4_698 0.42 -0.2 798,028 + Pf1N1B4_698 0.42 -0.6 798,029 - Pf1N1B4_698 0.42 -0.9 798,029 - Pf1N1B4_698 0.42 -0.6 798,029 - Pf1N1B4_698 0.42 -0.2
Or see this region's nucleotide sequence