Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_613

Experiment: Spectinomycin 0.05 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_611 and Pf1N1B4_612 are separated by 133 nucleotidesPf1N1B4_612 and Pf1N1B4_613 overlap by 4 nucleotidesPf1N1B4_613 and Pf1N1B4_614 are separated by 446 nucleotides Pf1N1B4_611 - CoA transferase, CAIB/BAIF family, at 705,554 to 706,885 _611 Pf1N1B4_612 - DNA-3-methyladenine glycosylase II (EC 3.2.2.21), at 707,019 to 707,882 _612 Pf1N1B4_613 - Arginine/ornithine antiporter ArcD, at 707,879 to 709,306 _613 Pf1N1B4_614 - Arginine/ornithine antiporter ArcD, at 709,753 to 711,180 _614 Position (kb) 707 708 709 710Strain fitness (log2 ratio) -2 -1 0 1 2 3at 706.997 kb on - strandat 707.063 kb on - strandat 707.209 kb on + strand, within Pf1N1B4_612at 707.210 kb on - strand, within Pf1N1B4_612at 707.210 kb on - strand, within Pf1N1B4_612at 707.210 kb on - strand, within Pf1N1B4_612at 707.210 kb on - strand, within Pf1N1B4_612at 707.210 kb on - strand, within Pf1N1B4_612at 707.397 kb on + strand, within Pf1N1B4_612at 707.397 kb on + strand, within Pf1N1B4_612at 707.398 kb on - strandat 707.398 kb on - strand, within Pf1N1B4_612at 707.398 kb on - strand, within Pf1N1B4_612at 707.398 kb on - strand, within Pf1N1B4_612at 707.398 kb on - strand, within Pf1N1B4_612at 707.398 kb on - strand, within Pf1N1B4_612at 707.777 kb on - strand, within Pf1N1B4_612at 707.851 kb on + strandat 707.852 kb on - strandat 707.901 kb on + strandat 707.902 kb on - strandat 707.902 kb on - strandat 707.902 kb on - strandat 707.902 kb on - strandat 707.902 kb on - strandat 707.902 kb on - strandat 707.969 kb on - strandat 708.027 kb on + strand, within Pf1N1B4_613at 708.028 kb on - strand, within Pf1N1B4_613at 708.070 kb on - strand, within Pf1N1B4_613at 708.070 kb on - strand, within Pf1N1B4_613at 708.070 kb on - strand, within Pf1N1B4_613at 708.070 kb on - strand, within Pf1N1B4_613at 708.148 kb on - strand, within Pf1N1B4_613at 708.148 kb on - strand, within Pf1N1B4_613at 708.148 kb on - strand, within Pf1N1B4_613at 708.148 kb on - strand, within Pf1N1B4_613at 708.148 kb on - strand, within Pf1N1B4_613at 708.181 kb on - strand, within Pf1N1B4_613at 708.199 kb on - strand, within Pf1N1B4_613at 708.199 kb on - strand, within Pf1N1B4_613at 708.199 kb on - strand, within Pf1N1B4_613at 708.199 kb on - strand, within Pf1N1B4_613at 708.228 kb on + strand, within Pf1N1B4_613at 708.228 kb on + strand, within Pf1N1B4_613at 708.229 kb on - strand, within Pf1N1B4_613at 708.229 kb on - strandat 708.243 kb on + strand, within Pf1N1B4_613at 708.243 kb on + strand, within Pf1N1B4_613at 708.243 kb on + strand, within Pf1N1B4_613at 708.243 kb on + strand, within Pf1N1B4_613at 708.243 kb on + strand, within Pf1N1B4_613at 708.243 kb on + strand, within Pf1N1B4_613at 708.243 kb on + strand, within Pf1N1B4_613at 708.244 kb on - strand, within Pf1N1B4_613at 708.244 kb on - strand, within Pf1N1B4_613at 708.244 kb on - strand, within Pf1N1B4_613at 708.244 kb on - strand, within Pf1N1B4_613at 708.437 kb on - strand, within Pf1N1B4_613at 708.481 kb on + strand, within Pf1N1B4_613at 708.481 kb on + strand, within Pf1N1B4_613at 708.481 kb on + strand, within Pf1N1B4_613at 708.482 kb on - strand, within Pf1N1B4_613at 708.592 kb on + strand, within Pf1N1B4_613at 708.649 kb on - strand, within Pf1N1B4_613at 709.054 kb on - strand, within Pf1N1B4_613at 709.054 kb on - strand, within Pf1N1B4_613at 709.054 kb on - strand, within Pf1N1B4_613at 709.054 kb on - strand, within Pf1N1B4_613at 709.083 kb on + strand, within Pf1N1B4_613at 709.083 kb on + strand, within Pf1N1B4_613at 709.083 kb on + strandat 709.083 kb on + strandat 709.083 kb on + strand, within Pf1N1B4_613at 709.084 kb on - strand, within Pf1N1B4_613at 709.084 kb on - strand, within Pf1N1B4_613at 709.084 kb on - strand, within Pf1N1B4_613at 709.084 kb on - strand, within Pf1N1B4_613at 709.084 kb on - strand, within Pf1N1B4_613at 709.084 kb on - strandat 709.084 kb on - strand, within Pf1N1B4_613at 709.335 kb on - strandat 709.374 kb on - strandat 709.385 kb on - strandat 709.422 kb on - strandat 709.425 kb on + strandat 709.426 kb on - strandat 709.473 kb on + strandat 709.473 kb on + strandat 709.474 kb on - strandat 709.529 kb on + strandat 709.540 kb on + strandat 709.541 kb on - strandat 709.541 kb on - strandat 709.643 kb on + strandat 709.643 kb on + strandat 709.652 kb on - strandat 709.882 kb on - strandat 709.976 kb on - strand, within Pf1N1B4_614at 710.005 kb on + strand, within Pf1N1B4_614at 710.065 kb on + strand, within Pf1N1B4_614at 710.065 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml
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706,997 - -0.9
707,063 - -1.7
707,209 + Pf1N1B4_612 0.22 +0.5
707,210 - Pf1N1B4_612 0.22 +0.7
707,210 - Pf1N1B4_612 0.22 -0.1
707,210 - Pf1N1B4_612 0.22 +0.1
707,210 - Pf1N1B4_612 0.22 +0.3
707,210 - Pf1N1B4_612 0.22 +3.7
707,397 + Pf1N1B4_612 0.44 +0.9
707,397 + Pf1N1B4_612 0.44 -1.1
707,398 - -0.1
707,398 - Pf1N1B4_612 0.44 -1.7
707,398 - Pf1N1B4_612 0.44 -2.1
707,398 - Pf1N1B4_612 0.44 -1.4
707,398 - Pf1N1B4_612 0.44 +2.0
707,398 - Pf1N1B4_612 0.44 -1.0
707,777 - Pf1N1B4_612 0.88 +1.1
707,851 + +0.6
707,852 - +0.3
707,901 + -0.1
707,902 - -0.1
707,902 - +0.2
707,902 - -1.9
707,902 - -0.1
707,902 - +0.3
707,902 - -0.0
707,969 - +0.7
708,027 + Pf1N1B4_613 0.10 -1.1
708,028 - Pf1N1B4_613 0.10 -0.9
708,070 - Pf1N1B4_613 0.13 +1.9
708,070 - Pf1N1B4_613 0.13 -0.1
708,070 - Pf1N1B4_613 0.13 +0.6
708,070 - Pf1N1B4_613 0.13 -0.8
708,148 - Pf1N1B4_613 0.19 +0.8
708,148 - Pf1N1B4_613 0.19 +0.5
708,148 - Pf1N1B4_613 0.19 +0.3
708,148 - Pf1N1B4_613 0.19 -2.1
708,148 - Pf1N1B4_613 0.19 -0.7
708,181 - Pf1N1B4_613 0.21 +0.3
708,199 - Pf1N1B4_613 0.22 +0.2
708,199 - Pf1N1B4_613 0.22 +0.3
708,199 - Pf1N1B4_613 0.22 -0.1
708,199 - Pf1N1B4_613 0.22 -0.3
708,228 + Pf1N1B4_613 0.24 -0.1
708,228 + Pf1N1B4_613 0.24 +0.6
708,229 - Pf1N1B4_613 0.25 +0.4
708,229 - +1.5
708,243 + Pf1N1B4_613 0.25 +0.3
708,243 + Pf1N1B4_613 0.25 -1.3
708,243 + Pf1N1B4_613 0.25 -0.1
708,243 + Pf1N1B4_613 0.25 -0.5
708,243 + Pf1N1B4_613 0.25 -1.4
708,243 + Pf1N1B4_613 0.25 -1.0
708,243 + Pf1N1B4_613 0.25 +0.8
708,244 - Pf1N1B4_613 0.26 -0.4
708,244 - Pf1N1B4_613 0.26 -1.3
708,244 - Pf1N1B4_613 0.26 -1.1
708,244 - Pf1N1B4_613 0.26 +0.9
708,437 - Pf1N1B4_613 0.39 +1.2
708,481 + Pf1N1B4_613 0.42 +0.2
708,481 + Pf1N1B4_613 0.42 +1.3
708,481 + Pf1N1B4_613 0.42 +2.5
708,482 - Pf1N1B4_613 0.42 +1.5
708,592 + Pf1N1B4_613 0.50 +0.6
708,649 - Pf1N1B4_613 0.54 -0.1
709,054 - Pf1N1B4_613 0.82 -1.7
709,054 - Pf1N1B4_613 0.82 -0.7
709,054 - Pf1N1B4_613 0.82 -1.7
709,054 - Pf1N1B4_613 0.82 -0.9
709,083 + Pf1N1B4_613 0.84 +0.3
709,083 + Pf1N1B4_613 0.84 +0.5
709,083 + -0.4
709,083 + -0.4
709,083 + Pf1N1B4_613 0.84 -0.9
709,084 - Pf1N1B4_613 0.84 +0.9
709,084 - Pf1N1B4_613 0.84 +0.3
709,084 - Pf1N1B4_613 0.84 +0.2
709,084 - Pf1N1B4_613 0.84 -2.4
709,084 - Pf1N1B4_613 0.84 -0.2
709,084 - +2.5
709,084 - Pf1N1B4_613 0.84 -1.4
709,335 - -0.7
709,374 - +1.2
709,385 - +0.8
709,422 - +0.2
709,425 + -1.1
709,426 - -0.1
709,473 + +0.9
709,473 + -0.9
709,474 - -0.1
709,529 + +0.9
709,540 + -1.4
709,541 - -0.1
709,541 - +1.1
709,643 + -0.4
709,643 + +0.9
709,652 - +0.0
709,882 - -1.7
709,976 - Pf1N1B4_614 0.16 -0.7
710,005 + Pf1N1B4_614 0.18 +0.2
710,065 + Pf1N1B4_614 0.22 +0.0
710,065 + +0.9

Or see this region's nucleotide sequence