Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5971 and Pf1N1B4_5972 overlap by 8 nucleotides Pf1N1B4_5972 and Pf1N1B4_5973 are separated by 10 nucleotides Pf1N1B4_5973 and Pf1N1B4_5974 overlap by 8 nucleotides
Pf1N1B4_5971 - 2-Methylcitrate dehydratase AcnD, at 418,726 to 420,504
_5971
Pf1N1B4_5972 - Ribose ABC transport system, permease protein RbsC (TC 3.A.1.2.1), at 420,497 to 421,162
_5972
Pf1N1B4_5973 - Probable permease of ABC transporter, at 421,173 to 421,823
_5973
Pf1N1B4_5974 - ABC-type polar amino acid transport system, ATPase component, at 421,816 to 422,538
_5974
Position (kb) 420
421
422 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 419.527 kb on + strand at 419.527 kb on + strand, within Pf1N1B4_5971 at 419.527 kb on + strand, within Pf1N1B4_5971 at 419.528 kb on - strand, within Pf1N1B4_5971 at 419.528 kb on - strand, within Pf1N1B4_5971 at 419.528 kb on - strand, within Pf1N1B4_5971 at 419.528 kb on - strand, within Pf1N1B4_5971 at 419.528 kb on - strand, within Pf1N1B4_5971 at 419.528 kb on - strand at 419.528 kb on - strand at 419.528 kb on - strand at 419.529 kb on + strand, within Pf1N1B4_5971 at 419.680 kb on + strand, within Pf1N1B4_5971 at 419.680 kb on + strand, within Pf1N1B4_5971 at 419.819 kb on - strand, within Pf1N1B4_5971 at 420.042 kb on - strand, within Pf1N1B4_5971 at 420.042 kb on - strand, within Pf1N1B4_5971 at 420.042 kb on - strand, within Pf1N1B4_5971 at 420.042 kb on - strand, within Pf1N1B4_5971 at 420.192 kb on + strand at 420.352 kb on + strand at 420.352 kb on + strand at 420.352 kb on + strand at 420.353 kb on - strand at 420.353 kb on - strand at 420.353 kb on - strand at 420.353 kb on - strand at 420.507 kb on - strand at 420.507 kb on - strand at 420.720 kb on - strand, within Pf1N1B4_5972 at 420.720 kb on - strand, within Pf1N1B4_5972 at 420.729 kb on - strand, within Pf1N1B4_5972 at 421.040 kb on + strand, within Pf1N1B4_5972 at 421.041 kb on - strand, within Pf1N1B4_5972 at 421.094 kb on + strand, within Pf1N1B4_5972 at 421.095 kb on - strand, within Pf1N1B4_5972 at 421.095 kb on - strand, within Pf1N1B4_5972 at 421.098 kb on - strand at 421.458 kb on + strand, within Pf1N1B4_5973 at 421.470 kb on + strand, within Pf1N1B4_5973 at 421.748 kb on + strand, within Pf1N1B4_5973 at 421.748 kb on + strand, within Pf1N1B4_5973 at 421.749 kb on - strand, within Pf1N1B4_5973 at 421.753 kb on - strand, within Pf1N1B4_5973 at 421.755 kb on + strand, within Pf1N1B4_5973 at 421.755 kb on + strand, within Pf1N1B4_5973 at 421.835 kb on + strand at 421.835 kb on + strand at 421.835 kb on + strand at 421.835 kb on + strand at 421.835 kb on + strand at 421.835 kb on + strand at 421.836 kb on - strand at 421.836 kb on - strand at 421.836 kb on - strand at 421.839 kb on + strand at 421.839 kb on + strand at 421.839 kb on + strand at 421.840 kb on - strand at 421.840 kb on - strand at 421.840 kb on - strand at 421.840 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 419,527 + -0.4 419,527 + Pf1N1B4_5971 0.45 -0.2 419,527 + Pf1N1B4_5971 0.45 -0.2 419,528 - Pf1N1B4_5971 0.45 +0.2 419,528 - Pf1N1B4_5971 0.45 -1.3 419,528 - Pf1N1B4_5971 0.45 -1.0 419,528 - Pf1N1B4_5971 0.45 +0.4 419,528 - Pf1N1B4_5971 0.45 -0.7 419,528 - +0.2 419,528 - +0.6 419,528 - +1.0 419,529 + Pf1N1B4_5971 0.45 +1.0 419,680 + Pf1N1B4_5971 0.54 -0.2 419,680 + Pf1N1B4_5971 0.54 +1.6 419,819 - Pf1N1B4_5971 0.61 +0.4 420,042 - Pf1N1B4_5971 0.74 -0.2 420,042 - Pf1N1B4_5971 0.74 -0.6 420,042 - Pf1N1B4_5971 0.74 -1.0 420,042 - Pf1N1B4_5971 0.74 +0.2 420,192 + +1.9 420,352 + -0.4 420,352 + +2.0 420,352 + -2.0 420,353 - +0.2 420,353 - -0.4 420,353 - -2.0 420,353 - +1.6 420,507 - -0.4 420,507 - +0.6 420,720 - Pf1N1B4_5972 0.33 -1.2 420,720 - Pf1N1B4_5972 0.33 -0.7 420,729 - Pf1N1B4_5972 0.35 -0.4 421,040 + Pf1N1B4_5972 0.82 -1.7 421,041 - Pf1N1B4_5972 0.82 -1.4 421,094 + Pf1N1B4_5972 0.90 -2.0 421,095 - Pf1N1B4_5972 0.90 -2.0 421,095 - Pf1N1B4_5972 0.90 -0.4 421,098 - -0.7 421,458 + Pf1N1B4_5973 0.44 +0.6 421,470 + Pf1N1B4_5973 0.46 +0.5 421,748 + Pf1N1B4_5973 0.88 -1.7 421,748 + Pf1N1B4_5973 0.88 +0.1 421,749 - Pf1N1B4_5973 0.88 +0.4 421,753 - Pf1N1B4_5973 0.89 +0.5 421,755 + Pf1N1B4_5973 0.89 +0.1 421,755 + Pf1N1B4_5973 0.89 +0.5 421,835 + -0.2 421,835 + -0.6 421,835 + -0.9 421,835 + -0.4 421,835 + -1.2 421,835 + -0.2 421,836 - +0.6 421,836 - +1.6 421,836 - +1.4 421,839 + +1.0 421,839 + -2.0 421,839 + +1.6 421,840 - +2.9 421,840 - -0.5 421,840 - -0.4 421,840 - -1.9
Or see this region's nucleotide sequence