Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5890 and Pf1N1B4_5891 overlap by 4 nucleotides Pf1N1B4_5891 and Pf1N1B4_5892 overlap by 34 nucleotides Pf1N1B4_5892 and Pf1N1B4_5893 overlap by 4 nucleotides
Pf1N1B4_5890 - Glycosyltransferase, at 333,753 to 334,856
_5890
Pf1N1B4_5891 - FIG00961753: hypothetical protein, at 334,853 to 336,067
_5891
Pf1N1B4_5892 - FIG00961753: hypothetical protein, at 336,034 to 336,216
_5892
Pf1N1B4_5893 - Mycofactocin system glycosyltransferase, at 336,213 to 337,190
_5893
Position (kb) 334
335
336
337 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 334.042 kb on + strand at 334.131 kb on + strand, within Pf1N1B4_5890 at 334.323 kb on + strand, within Pf1N1B4_5890 at 334.770 kb on - strand at 334.825 kb on - strand at 334.825 kb on - strand at 334.825 kb on - strand at 334.825 kb on - strand at 335.177 kb on + strand, within Pf1N1B4_5891 at 335.299 kb on + strand at 335.682 kb on - strand, within Pf1N1B4_5891 at 335.779 kb on + strand at 335.928 kb on + strand at 335.928 kb on + strand, within Pf1N1B4_5891 at 335.929 kb on - strand, within Pf1N1B4_5891 at 335.929 kb on - strand, within Pf1N1B4_5891 at 335.929 kb on - strand, within Pf1N1B4_5891 at 335.932 kb on + strand, within Pf1N1B4_5891 at 335.932 kb on + strand, within Pf1N1B4_5891 at 336.004 kb on + strand at 336.004 kb on + strand at 336.004 kb on + strand at 336.004 kb on + strand at 336.005 kb on - strand at 336.088 kb on + strand, within Pf1N1B4_5892 at 336.088 kb on + strand, within Pf1N1B4_5892 at 336.088 kb on + strand, within Pf1N1B4_5892 at 336.088 kb on + strand, within Pf1N1B4_5892 at 336.088 kb on + strand at 336.088 kb on + strand, within Pf1N1B4_5892 at 336.088 kb on + strand, within Pf1N1B4_5892 at 336.089 kb on - strand, within Pf1N1B4_5892 at 336.089 kb on - strand, within Pf1N1B4_5892 at 336.089 kb on - strand, within Pf1N1B4_5892 at 336.089 kb on - strand, within Pf1N1B4_5892 at 336.139 kb on + strand, within Pf1N1B4_5892 at 336.252 kb on + strand at 336.252 kb on + strand at 336.279 kb on - strand at 337.015 kb on + strand, within Pf1N1B4_5893 at 337.016 kb on - strand, within Pf1N1B4_5893
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 334,042 + +1.0 334,131 + Pf1N1B4_5890 0.34 -2.6 334,323 + Pf1N1B4_5890 0.52 -2.6 334,770 - -0.1 334,825 - +1.6 334,825 - -0.7 334,825 - +0.3 334,825 - -0.2 335,177 + Pf1N1B4_5891 0.27 -2.2 335,299 + -1.4 335,682 - Pf1N1B4_5891 0.68 -0.6 335,779 + -2.2 335,928 + -1.4 335,928 + Pf1N1B4_5891 0.88 -0.8 335,929 - Pf1N1B4_5891 0.89 -1.2 335,929 - Pf1N1B4_5891 0.89 -0.2 335,929 - Pf1N1B4_5891 0.89 -1.2 335,932 + Pf1N1B4_5891 0.89 +0.6 335,932 + Pf1N1B4_5891 0.89 -1.0 336,004 + +1.5 336,004 + -0.3 336,004 + +0.3 336,004 + +1.2 336,005 - -3.1 336,088 + Pf1N1B4_5892 0.30 -2.0 336,088 + Pf1N1B4_5892 0.30 -0.7 336,088 + Pf1N1B4_5892 0.30 -0.6 336,088 + Pf1N1B4_5892 0.30 -0.7 336,088 + +1.0 336,088 + Pf1N1B4_5892 0.30 +1.1 336,088 + Pf1N1B4_5892 0.30 -2.2 336,089 - Pf1N1B4_5892 0.30 +2.2 336,089 - Pf1N1B4_5892 0.30 -0.6 336,089 - Pf1N1B4_5892 0.30 +1.9 336,089 - Pf1N1B4_5892 0.30 +0.3 336,139 + Pf1N1B4_5892 0.57 -0.7 336,252 + +0.2 336,252 + -1.4 336,279 - -0.0 337,015 + Pf1N1B4_5893 0.82 +0.6 337,016 - Pf1N1B4_5893 0.82 +0.4
Or see this region's nucleotide sequence