Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5723 and Pf1N1B4_5724 are separated by 34 nucleotides Pf1N1B4_5724 and Pf1N1B4_5725 overlap by 16 nucleotides Pf1N1B4_5725 and Pf1N1B4_5726 are separated by 38 nucleotides Pf1N1B4_5726 and Pf1N1B4_5727 are separated by 15 nucleotides Pf1N1B4_5727 and Pf1N1B4_5728 are separated by 5 nucleotides Pf1N1B4_5728 and Pf1N1B4_5729 are separated by 32 nucleotides
Pf1N1B4_5723 - putative exported protein, at 164,092 to 164,424
_5723
Pf1N1B4_5724 - High-affinity iron permease, at 164,459 to 164,941
_5724
Pf1N1B4_5725 - High-affinity iron permease, at 164,926 to 165,300
_5725
Pf1N1B4_5726 - Ferredoxin, at 165,339 to 166,667
_5726
Pf1N1B4_5727 - Ribonucleotide reductase of class III (anaerobic), activating protein (EC 1.97.1.4), at 166,683 to 167,369
_5727
Pf1N1B4_5728 - hypothetical protein, at 167,375 to 167,542
_5728
Pf1N1B4_5729 - Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2), at 167,575 to 169,590
_5729
Position (kb) 165
166
167 Strain fitness (log2 ratio) -1
0
1
2 at 164.798 kb on + strand, within Pf1N1B4_5724 at 164.798 kb on + strand, within Pf1N1B4_5724 at 164.798 kb on + strand, within Pf1N1B4_5724 at 164.801 kb on + strand, within Pf1N1B4_5724 at 164.802 kb on - strand, within Pf1N1B4_5724 at 165.101 kb on - strand, within Pf1N1B4_5725 at 165.385 kb on + strand at 165.394 kb on - strand at 165.485 kb on - strand, within Pf1N1B4_5726 at 165.594 kb on + strand, within Pf1N1B4_5726 at 165.699 kb on + strand, within Pf1N1B4_5726 at 166.054 kb on + strand, within Pf1N1B4_5726 at 166.374 kb on + strand at 166.511 kb on - strand, within Pf1N1B4_5726 at 166.511 kb on - strand, within Pf1N1B4_5726 at 166.906 kb on + strand, within Pf1N1B4_5727
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 164,798 + Pf1N1B4_5724 0.70 -0.8 164,798 + Pf1N1B4_5724 0.70 -0.7 164,798 + Pf1N1B4_5724 0.70 -0.6 164,801 + Pf1N1B4_5724 0.71 +0.2 164,802 - Pf1N1B4_5724 0.71 -1.3 165,101 - Pf1N1B4_5725 0.47 -0.8 165,385 + +0.6 165,394 - +0.8 165,485 - Pf1N1B4_5726 0.11 +1.9 165,594 + Pf1N1B4_5726 0.19 -0.5 165,699 + Pf1N1B4_5726 0.27 +0.5 166,054 + Pf1N1B4_5726 0.54 -1.6 166,374 + +0.5 166,511 - Pf1N1B4_5726 0.88 -0.9 166,511 - Pf1N1B4_5726 0.88 +0.1 166,906 + Pf1N1B4_5727 0.32 -1.1
Or see this region's nucleotide sequence