Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5716

Experiment: Spectinomycin 0.05 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5715 and Pf1N1B4_5716 overlap by 4 nucleotidesPf1N1B4_5716 and Pf1N1B4_5717 are separated by 159 nucleotidesPf1N1B4_5717 and Pf1N1B4_5718 are separated by 100 nucleotides Pf1N1B4_5715 - RND efflux membrane fusion protein, at 156,720 to 157,754 _5715 Pf1N1B4_5716 - Acriflavin resistance protein, at 157,751 to 160,846 _5716 Pf1N1B4_5717 - hypothetical protein, at 161,006 to 161,122 _5717 Pf1N1B4_5718 - Transcriptional regulator, Cro/CI family, at 161,223 to 162,014 _5718 Position (kb) 157 158 159 160 161Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 156.799 kb on + strandat 156.868 kb on + strand, within Pf1N1B4_5715at 157.061 kb on + strand, within Pf1N1B4_5715at 157.062 kb on - strand, within Pf1N1B4_5715at 157.062 kb on - strand, within Pf1N1B4_5715at 157.062 kb on - strand, within Pf1N1B4_5715at 157.062 kb on - strand, within Pf1N1B4_5715at 157.137 kb on + strand, within Pf1N1B4_5715at 157.137 kb on + strand, within Pf1N1B4_5715at 157.138 kb on - strand, within Pf1N1B4_5715at 157.138 kb on - strandat 157.396 kb on - strand, within Pf1N1B4_5715at 157.691 kb on + strandat 158.174 kb on + strand, within Pf1N1B4_5716at 158.570 kb on + strand, within Pf1N1B4_5716at 158.570 kb on + strandat 159.038 kb on + strand, within Pf1N1B4_5716at 159.206 kb on + strand, within Pf1N1B4_5716at 159.259 kb on + strand, within Pf1N1B4_5716at 159.259 kb on + strand, within Pf1N1B4_5716at 159.259 kb on + strand, within Pf1N1B4_5716at 159.260 kb on - strand, within Pf1N1B4_5716at 159.260 kb on - strandat 159.260 kb on - strand, within Pf1N1B4_5716at 159.260 kb on - strand, within Pf1N1B4_5716at 159.263 kb on + strand, within Pf1N1B4_5716at 159.263 kb on + strand, within Pf1N1B4_5716at 159.263 kb on + strand, within Pf1N1B4_5716at 159.263 kb on + strand, within Pf1N1B4_5716at 159.263 kb on + strand, within Pf1N1B4_5716at 159.263 kb on + strand, within Pf1N1B4_5716at 159.263 kb on + strand, within Pf1N1B4_5716at 159.263 kb on + strand, within Pf1N1B4_5716at 159.263 kb on + strand, within Pf1N1B4_5716at 159.264 kb on - strand, within Pf1N1B4_5716at 159.264 kb on - strand, within Pf1N1B4_5716at 159.264 kb on - strandat 159.264 kb on - strand, within Pf1N1B4_5716at 159.264 kb on - strandat 159.264 kb on - strand, within Pf1N1B4_5716at 159.278 kb on + strand, within Pf1N1B4_5716at 159.527 kb on + strandat 159.527 kb on + strand, within Pf1N1B4_5716at 159.527 kb on + strandat 159.564 kb on - strand, within Pf1N1B4_5716at 159.615 kb on + strand, within Pf1N1B4_5716at 159.616 kb on - strand, within Pf1N1B4_5716at 159.616 kb on - strand, within Pf1N1B4_5716at 159.810 kb on + strand, within Pf1N1B4_5716at 159.810 kb on + strand, within Pf1N1B4_5716at 159.810 kb on + strand, within Pf1N1B4_5716at 159.810 kb on + strand, within Pf1N1B4_5716at 159.811 kb on - strand, within Pf1N1B4_5716at 159.811 kb on - strand, within Pf1N1B4_5716at 160.067 kb on + strand, within Pf1N1B4_5716at 160.207 kb on - strand, within Pf1N1B4_5716at 160.369 kb on + strand, within Pf1N1B4_5716at 160.369 kb on + strand, within Pf1N1B4_5716at 160.369 kb on + strand, within Pf1N1B4_5716at 160.369 kb on + strandat 160.369 kb on + strand, within Pf1N1B4_5716at 160.369 kb on + strand, within Pf1N1B4_5716at 160.370 kb on - strand, within Pf1N1B4_5716at 160.370 kb on - strand, within Pf1N1B4_5716at 160.370 kb on - strand, within Pf1N1B4_5716at 160.370 kb on - strand, within Pf1N1B4_5716at 160.370 kb on - strand, within Pf1N1B4_5716at 160.763 kb on + strandat 160.763 kb on + strandat 160.763 kb on + strandat 160.764 kb on - strandat 160.882 kb on + strandat 160.882 kb on + strandat 160.883 kb on - strandat 160.885 kb on + strandat 160.885 kb on + strandat 160.885 kb on + strandat 160.885 kb on + strandat 160.885 kb on + strandat 160.886 kb on - strandat 160.886 kb on - strandat 160.886 kb on - strandat 160.886 kb on - strandat 160.886 kb on - strandat 161.172 kb on - strandat 161.283 kb on - strandat 161.283 kb on - strandat 161.287 kb on - strandat 161.530 kb on + strand, within Pf1N1B4_5718at 161.530 kb on + strandat 161.589 kb on + strand, within Pf1N1B4_5718at 161.589 kb on + strand, within Pf1N1B4_5718at 161.696 kb on + strand, within Pf1N1B4_5718at 161.832 kb on + strand, within Pf1N1B4_5718at 161.832 kb on + strand, within Pf1N1B4_5718at 161.833 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml
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156,799 + -1.3
156,868 + Pf1N1B4_5715 0.14 +0.6
157,061 + Pf1N1B4_5715 0.33 -1.3
157,062 - Pf1N1B4_5715 0.33 +0.2
157,062 - Pf1N1B4_5715 0.33 -0.1
157,062 - Pf1N1B4_5715 0.33 +0.5
157,062 - Pf1N1B4_5715 0.33 +1.1
157,137 + Pf1N1B4_5715 0.40 -1.1
157,137 + Pf1N1B4_5715 0.40 -0.5
157,138 - Pf1N1B4_5715 0.40 -0.1
157,138 - +2.3
157,396 - Pf1N1B4_5715 0.65 -0.5
157,691 + -0.5
158,174 + Pf1N1B4_5716 0.14 +0.2
158,570 + Pf1N1B4_5716 0.26 +0.5
158,570 + -0.5
159,038 + Pf1N1B4_5716 0.42 -0.6
159,206 + Pf1N1B4_5716 0.47 -1.1
159,259 + Pf1N1B4_5716 0.49 -0.6
159,259 + Pf1N1B4_5716 0.49 -0.8
159,259 + Pf1N1B4_5716 0.49 +1.8
159,260 - Pf1N1B4_5716 0.49 -0.5
159,260 - +0.2
159,260 - Pf1N1B4_5716 0.49 -0.1
159,260 - Pf1N1B4_5716 0.49 -0.5
159,263 + Pf1N1B4_5716 0.49 -0.5
159,263 + Pf1N1B4_5716 0.49 +0.9
159,263 + Pf1N1B4_5716 0.49 +0.8
159,263 + Pf1N1B4_5716 0.49 +0.4
159,263 + Pf1N1B4_5716 0.49 +0.4
159,263 + Pf1N1B4_5716 0.49 -0.8
159,263 + Pf1N1B4_5716 0.49 +0.0
159,263 + Pf1N1B4_5716 0.49 -0.3
159,263 + Pf1N1B4_5716 0.49 -1.5
159,264 - Pf1N1B4_5716 0.49 +0.5
159,264 - Pf1N1B4_5716 0.49 +1.1
159,264 - -0.2
159,264 - Pf1N1B4_5716 0.49 -2.3
159,264 - -0.8
159,264 - Pf1N1B4_5716 0.49 +1.6
159,278 + Pf1N1B4_5716 0.49 +0.1
159,527 + +2.1
159,527 + Pf1N1B4_5716 0.57 +0.1
159,527 + -0.5
159,564 - Pf1N1B4_5716 0.59 +0.2
159,615 + Pf1N1B4_5716 0.60 -1.1
159,616 - Pf1N1B4_5716 0.60 -0.8
159,616 - Pf1N1B4_5716 0.60 +0.5
159,810 + Pf1N1B4_5716 0.67 -2.9
159,810 + Pf1N1B4_5716 0.67 -1.1
159,810 + Pf1N1B4_5716 0.67 -0.3
159,810 + Pf1N1B4_5716 0.67 -0.0
159,811 - Pf1N1B4_5716 0.67 -0.1
159,811 - Pf1N1B4_5716 0.67 +0.7
160,067 + Pf1N1B4_5716 0.75 +0.4
160,207 - Pf1N1B4_5716 0.79 -0.8
160,369 + Pf1N1B4_5716 0.85 -1.0
160,369 + Pf1N1B4_5716 0.85 +0.2
160,369 + Pf1N1B4_5716 0.85 -1.0
160,369 + -0.1
160,369 + Pf1N1B4_5716 0.85 -0.7
160,369 + Pf1N1B4_5716 0.85 -0.3
160,370 - Pf1N1B4_5716 0.85 -1.8
160,370 - Pf1N1B4_5716 0.85 -0.2
160,370 - Pf1N1B4_5716 0.85 +0.5
160,370 - Pf1N1B4_5716 0.85 -2.5
160,370 - Pf1N1B4_5716 0.85 -1.8
160,763 + +0.3
160,763 + +0.5
160,763 + -0.1
160,764 - -0.5
160,882 + -1.5
160,882 + +0.9
160,883 - +0.8
160,885 + +0.5
160,885 + -1.7
160,885 + -0.5
160,885 + +0.3
160,885 + +0.3
160,886 - -3.3
160,886 - -0.7
160,886 - +1.1
160,886 - -1.9
160,886 - +0.5
161,172 - +1.1
161,283 - -1.8
161,283 - +1.0
161,287 - -0.2
161,530 + Pf1N1B4_5718 0.39 +1.1
161,530 + +0.5
161,589 + Pf1N1B4_5718 0.46 +1.5
161,589 + Pf1N1B4_5718 0.46 +0.5
161,696 + Pf1N1B4_5718 0.60 -0.8
161,832 + Pf1N1B4_5718 0.77 -0.0
161,832 + Pf1N1B4_5718 0.77 +0.5
161,833 - -1.1

Or see this region's nucleotide sequence