Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5706 and Pf1N1B4_5707 are separated by 10 nucleotides Pf1N1B4_5707 and Pf1N1B4_5708 are separated by 84 nucleotides
Pf1N1B4_5706 - Fumarylacetoacetate hydrolase family protein, at 144,539 to 145,660
_5706
Pf1N1B4_5707 - NADH dehydrogenase (EC 1.6.99.3), at 145,671 to 146,762
_5707
Pf1N1B4_5708 - Transcriptional regulator, TetR family, at 146,847 to 147,461
_5708
Position (kb) 145
146
147 Strain fitness (log2 ratio) -2
-1
0
1
2 at 144.884 kb on + strand, within Pf1N1B4_5706 at 144.885 kb on - strand, within Pf1N1B4_5706 at 144.910 kb on + strand, within Pf1N1B4_5706 at 144.978 kb on + strand, within Pf1N1B4_5706 at 145.436 kb on + strand, within Pf1N1B4_5706 at 145.479 kb on - strand, within Pf1N1B4_5706 at 145.479 kb on - strand, within Pf1N1B4_5706 at 145.484 kb on + strand, within Pf1N1B4_5706 at 145.502 kb on + strand, within Pf1N1B4_5706 at 145.639 kb on - strand at 145.847 kb on + strand, within Pf1N1B4_5707 at 146.047 kb on + strand, within Pf1N1B4_5707 at 146.048 kb on - strand, within Pf1N1B4_5707 at 146.048 kb on - strand, within Pf1N1B4_5707 at 146.048 kb on - strand at 146.082 kb on - strand, within Pf1N1B4_5707 at 146.132 kb on + strand, within Pf1N1B4_5707 at 146.132 kb on + strand, within Pf1N1B4_5707 at 146.132 kb on + strand, within Pf1N1B4_5707 at 146.133 kb on - strand, within Pf1N1B4_5707 at 146.133 kb on - strand, within Pf1N1B4_5707 at 146.151 kb on + strand, within Pf1N1B4_5707 at 146.152 kb on - strand, within Pf1N1B4_5707 at 146.190 kb on - strand, within Pf1N1B4_5707 at 146.348 kb on + strand, within Pf1N1B4_5707 at 146.348 kb on + strand, within Pf1N1B4_5707 at 146.349 kb on - strand at 146.549 kb on - strand at 146.555 kb on - strand, within Pf1N1B4_5707 at 146.555 kb on - strand, within Pf1N1B4_5707 at 146.582 kb on - strand, within Pf1N1B4_5707 at 146.790 kb on - strand at 147.330 kb on - strand, within Pf1N1B4_5708 at 147.332 kb on - strand, within Pf1N1B4_5708 at 147.332 kb on - strand, within Pf1N1B4_5708 at 147.480 kb on - strand at 147.567 kb on + strand at 147.567 kb on + strand at 147.567 kb on + strand at 147.590 kb on + strand at 147.591 kb on - strand at 147.591 kb on - strand at 147.591 kb on - strand at 147.623 kb on + strand at 147.747 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 144,884 + Pf1N1B4_5706 0.31 +0.1 144,885 - Pf1N1B4_5706 0.31 +1.0 144,910 + Pf1N1B4_5706 0.33 -1.1 144,978 + Pf1N1B4_5706 0.39 +0.1 145,436 + Pf1N1B4_5706 0.80 +0.2 145,479 - Pf1N1B4_5706 0.84 -2.1 145,479 - Pf1N1B4_5706 0.84 -1.2 145,484 + Pf1N1B4_5706 0.84 +0.9 145,502 + Pf1N1B4_5706 0.86 -0.2 145,639 - +0.0 145,847 + Pf1N1B4_5707 0.16 -1.8 146,047 + Pf1N1B4_5707 0.34 +0.5 146,048 - Pf1N1B4_5707 0.35 +0.9 146,048 - Pf1N1B4_5707 0.35 -0.4 146,048 - +0.5 146,082 - Pf1N1B4_5707 0.38 -2.3 146,132 + Pf1N1B4_5707 0.42 -0.5 146,132 + Pf1N1B4_5707 0.42 +0.9 146,132 + Pf1N1B4_5707 0.42 +0.9 146,133 - Pf1N1B4_5707 0.42 +0.1 146,133 - Pf1N1B4_5707 0.42 +0.1 146,151 + Pf1N1B4_5707 0.44 -0.3 146,152 - Pf1N1B4_5707 0.44 +0.9 146,190 - Pf1N1B4_5707 0.48 -2.1 146,348 + Pf1N1B4_5707 0.62 -0.2 146,348 + Pf1N1B4_5707 0.62 +1.9 146,349 - +2.3 146,549 - +0.5 146,555 - Pf1N1B4_5707 0.81 -0.1 146,555 - Pf1N1B4_5707 0.81 -0.1 146,582 - Pf1N1B4_5707 0.83 +0.2 146,790 - -0.5 147,330 - Pf1N1B4_5708 0.79 +1.5 147,332 - Pf1N1B4_5708 0.79 +0.1 147,332 - Pf1N1B4_5708 0.79 -0.5 147,480 - +0.7 147,567 + -0.3 147,567 + +1.5 147,567 + -1.5 147,590 + +1.9 147,591 - -1.8 147,591 - -1.5 147,591 - +0.5 147,623 + -2.1 147,747 - -1.7
Or see this region's nucleotide sequence