Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5687 and Pf1N1B4_5688 are separated by 57 nucleotides Pf1N1B4_5688 and Pf1N1B4_5689 are separated by 11 nucleotides Pf1N1B4_5689 and Pf1N1B4_5690 are separated by 70 nucleotides Pf1N1B4_5690 and Pf1N1B4_6053 are separated by 79 nucleotides Pf1N1B4_6053 and Pf1N1B4_5691 are separated by 507 nucleotides
Pf1N1B4_5687 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 126,372 to 126,809
_5687
Pf1N1B4_5688 - hypothetical protein, at 126,867 to 127,196
_5688
Pf1N1B4_5689 - Lactoylglutathione lyase, at 127,208 to 127,600
_5689
Pf1N1B4_5690 - Putative TEGT family carrier/transport protein, at 127,671 to 128,387
_5690
Pf1N1B4_6053 - tRNA-Ser-TGA, at 128,467 to 128,551
_6053
Pf1N1B4_5691 - Chromosome initiation inhibitor, at 129,059 to 130,000
_5691
Position (kb)
127
128
129 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 126.834 kb on - strand at 126.901 kb on + strand, within Pf1N1B4_5688 at 127.005 kb on + strand, within Pf1N1B4_5688 at 127.005 kb on + strand, within Pf1N1B4_5688 at 127.005 kb on + strand, within Pf1N1B4_5688 at 127.006 kb on - strand at 127.020 kb on + strand, within Pf1N1B4_5688 at 127.068 kb on + strand, within Pf1N1B4_5688 at 127.069 kb on - strand, within Pf1N1B4_5688 at 127.074 kb on + strand at 127.074 kb on + strand, within Pf1N1B4_5688 at 127.075 kb on - strand, within Pf1N1B4_5688 at 127.075 kb on - strand, within Pf1N1B4_5688 at 127.096 kb on + strand, within Pf1N1B4_5688 at 127.097 kb on - strand, within Pf1N1B4_5688 at 127.109 kb on - strand, within Pf1N1B4_5688 at 127.155 kb on + strand, within Pf1N1B4_5688 at 127.255 kb on - strand at 127.263 kb on - strand, within Pf1N1B4_5689 at 127.460 kb on + strand, within Pf1N1B4_5689 at 127.544 kb on + strand, within Pf1N1B4_5689 at 127.545 kb on - strand, within Pf1N1B4_5689 at 127.545 kb on - strand, within Pf1N1B4_5689 at 127.545 kb on - strand, within Pf1N1B4_5689 at 127.545 kb on - strand at 127.546 kb on + strand, within Pf1N1B4_5689 at 127.546 kb on + strand, within Pf1N1B4_5689 at 127.546 kb on + strand, within Pf1N1B4_5689 at 127.630 kb on - strand at 127.727 kb on - strand at 127.727 kb on - strand at 127.735 kb on - strand at 127.735 kb on - strand at 127.739 kb on - strand at 127.763 kb on - strand, within Pf1N1B4_5690 at 127.763 kb on - strand, within Pf1N1B4_5690 at 127.966 kb on + strand, within Pf1N1B4_5690 at 127.966 kb on + strand, within Pf1N1B4_5690 at 127.967 kb on - strand, within Pf1N1B4_5690 at 128.292 kb on + strand, within Pf1N1B4_5690 at 128.615 kb on - strand at 128.654 kb on - strand at 128.706 kb on - strand at 128.864 kb on - strand at 128.864 kb on - strand at 128.864 kb on - strand at 128.864 kb on - strand at 128.882 kb on - strand at 128.903 kb on - strand at 128.903 kb on - strand at 129.056 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 126,834 - -1.1 126,901 + Pf1N1B4_5688 0.10 +1.4 127,005 + Pf1N1B4_5688 0.42 -1.5 127,005 + Pf1N1B4_5688 0.42 -0.5 127,005 + Pf1N1B4_5688 0.42 +2.3 127,006 - -1.5 127,020 + Pf1N1B4_5688 0.46 +0.9 127,068 + Pf1N1B4_5688 0.61 -1.8 127,069 - Pf1N1B4_5688 0.61 +0.0 127,074 + -0.2 127,074 + Pf1N1B4_5688 0.63 -2.9 127,075 - Pf1N1B4_5688 0.63 -0.8 127,075 - Pf1N1B4_5688 0.63 -1.1 127,096 + Pf1N1B4_5688 0.69 -0.4 127,097 - Pf1N1B4_5688 0.70 -2.6 127,109 - Pf1N1B4_5688 0.73 -0.2 127,155 + Pf1N1B4_5688 0.87 +0.7 127,255 - +0.5 127,263 - Pf1N1B4_5689 0.14 +0.9 127,460 + Pf1N1B4_5689 0.64 +0.0 127,544 + Pf1N1B4_5689 0.85 +0.2 127,545 - Pf1N1B4_5689 0.86 -0.1 127,545 - Pf1N1B4_5689 0.86 +0.3 127,545 - Pf1N1B4_5689 0.86 -0.1 127,545 - +2.1 127,546 + Pf1N1B4_5689 0.86 -0.5 127,546 + Pf1N1B4_5689 0.86 -0.8 127,546 + Pf1N1B4_5689 0.86 -1.4 127,630 - +0.8 127,727 - -1.2 127,727 - -0.5 127,735 - +0.1 127,735 - -1.9 127,739 - -1.5 127,763 - Pf1N1B4_5690 0.13 -1.6 127,763 - Pf1N1B4_5690 0.13 -0.5 127,966 + Pf1N1B4_5690 0.41 -2.5 127,966 + Pf1N1B4_5690 0.41 -3.5 127,967 - Pf1N1B4_5690 0.41 -2.3 128,292 + Pf1N1B4_5690 0.87 -1.5 128,615 - -0.8 128,654 - -1.2 128,706 - -0.1 128,864 - -1.5 128,864 - +0.1 128,864 - -0.3 128,864 - +0.0 128,882 - -1.1 128,903 - +1.5 128,903 - +1.2 129,056 - -0.5
Or see this region's nucleotide sequence