Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5667 and Pf1N1B4_5668 are separated by 191 nucleotides Pf1N1B4_5668 and Pf1N1B4_5669 are separated by 202 nucleotides Pf1N1B4_5669 and Pf1N1B4_5670 are separated by 64 nucleotides
Pf1N1B4_5667 - hypothetical protein, at 100,927 to 104,862
_5667
Pf1N1B4_5668 - Peptidase, U7 family, at 105,054 to 106,076
_5668
Pf1N1B4_5669 - Phosphoglycerate mutase family protein, at 106,279 to 106,989
_5669
Pf1N1B4_5670 - SCP-2 sterol transfer family protein, at 107,054 to 107,368
_5670
Position (kb)
105
106
107 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 104.071 kb on - strand, within Pf1N1B4_5667 at 104.071 kb on - strand, within Pf1N1B4_5667 at 104.336 kb on + strand at 104.336 kb on + strand, within Pf1N1B4_5667 at 104.336 kb on + strand, within Pf1N1B4_5667 at 104.337 kb on - strand, within Pf1N1B4_5667 at 104.337 kb on - strand, within Pf1N1B4_5667 at 104.337 kb on - strand, within Pf1N1B4_5667 at 104.337 kb on - strand, within Pf1N1B4_5667 at 104.459 kb on + strand, within Pf1N1B4_5667 at 104.459 kb on + strand at 104.460 kb on - strand, within Pf1N1B4_5667 at 104.460 kb on - strand, within Pf1N1B4_5667 at 104.546 kb on + strand at 104.594 kb on + strand at 104.595 kb on - strand at 104.678 kb on + strand at 104.678 kb on + strand at 104.678 kb on + strand at 104.678 kb on + strand at 104.678 kb on + strand at 104.678 kb on + strand at 104.678 kb on + strand at 104.678 kb on + strand at 104.678 kb on + strand at 104.678 kb on + strand at 104.679 kb on - strand at 104.679 kb on - strand at 104.679 kb on - strand at 104.682 kb on + strand at 104.683 kb on - strand at 104.683 kb on - strand at 104.683 kb on - strand at 104.685 kb on - strand at 104.736 kb on + strand at 104.899 kb on + strand at 104.921 kb on - strand at 105.056 kb on - strand at 105.208 kb on + strand, within Pf1N1B4_5668 at 105.208 kb on + strand, within Pf1N1B4_5668 at 105.209 kb on - strand at 105.285 kb on + strand at 105.286 kb on - strand, within Pf1N1B4_5668 at 105.566 kb on - strand, within Pf1N1B4_5668 at 106.291 kb on + strand at 106.291 kb on + strand at 106.333 kb on + strand at 106.333 kb on + strand at 107.030 kb on + strand at 107.030 kb on + strand at 107.030 kb on + strand at 107.030 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 104,071 - Pf1N1B4_5667 0.80 +1.1 104,071 - Pf1N1B4_5667 0.80 +0.1 104,336 + -0.5 104,336 + Pf1N1B4_5667 0.87 +0.5 104,336 + Pf1N1B4_5667 0.87 -0.7 104,337 - Pf1N1B4_5667 0.87 +0.9 104,337 - Pf1N1B4_5667 0.87 -1.3 104,337 - Pf1N1B4_5667 0.87 +0.5 104,337 - Pf1N1B4_5667 0.87 -2.5 104,459 + Pf1N1B4_5667 0.90 -1.8 104,459 + +1.5 104,460 - Pf1N1B4_5667 0.90 +0.5 104,460 - Pf1N1B4_5667 0.90 +0.1 104,546 + -0.2 104,594 + -1.1 104,595 - +0.8 104,678 + +0.0 104,678 + +0.0 104,678 + -1.8 104,678 + -0.5 104,678 + -0.5 104,678 + -1.1 104,678 + +0.3 104,678 + +1.5 104,678 + -0.1 104,678 + +2.1 104,679 - -1.1 104,679 - +0.1 104,679 - +1.0 104,682 + +0.3 104,683 - +0.5 104,683 - -0.8 104,683 - -0.1 104,685 - +0.2 104,736 + -0.5 104,899 + -0.2 104,921 - -0.5 105,056 - +0.8 105,208 + Pf1N1B4_5668 0.15 +0.5 105,208 + Pf1N1B4_5668 0.15 -1.1 105,209 - +1.1 105,285 + -0.1 105,286 - Pf1N1B4_5668 0.23 -1.1 105,566 - Pf1N1B4_5668 0.50 -2.1 106,291 + -0.3 106,291 + +2.5 106,333 + -1.6 106,333 + +0.5 107,030 + -2.1 107,030 + -1.1 107,030 + -0.1 107,030 + -0.7
Or see this region's nucleotide sequence