Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5658

Experiment: Spectinomycin 0.05 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5656 and Pf1N1B4_5657 overlap by 4 nucleotidesPf1N1B4_5657 and Pf1N1B4_5658 are separated by 82 nucleotidesPf1N1B4_5658 and Pf1N1B4_5659 are separated by 183 nucleotidesPf1N1B4_5659 and Pf1N1B4_5660 are separated by 213 nucleotides Pf1N1B4_5656 - Predicted cobalt transporter CbtA, at 87,675 to 88,382 _5656 Pf1N1B4_5657 - Cobalt-precorrin-3b C17-methyltransferase, at 88,379 to 88,792 _5657 Pf1N1B4_5658 - Cobalt-precorrin-4 C11-methyltransferase (EC 2.1.1.133), at 88,875 to 89,621 _5658 Pf1N1B4_5659 - NfuA Fe-S protein maturation, at 89,805 to 90,389 _5659 Pf1N1B4_5660 - cis/trans isomerase, at 90,603 to 92,888 _5660 Position (kb) 88 89 90Strain fitness (log2 ratio) -2 -1 0 1 2at 87.986 kb on + strandat 87.986 kb on + strand, within Pf1N1B4_5656at 88.372 kb on - strandat 88.884 kb on + strandat 88.885 kb on - strandat 88.968 kb on + strand, within Pf1N1B4_5658at 88.968 kb on + strand, within Pf1N1B4_5658at 88.968 kb on + strand, within Pf1N1B4_5658at 88.968 kb on + strand, within Pf1N1B4_5658at 88.968 kb on + strand, within Pf1N1B4_5658at 88.969 kb on - strandat 88.969 kb on - strand, within Pf1N1B4_5658at 88.969 kb on - strand, within Pf1N1B4_5658at 88.969 kb on - strand, within Pf1N1B4_5658at 88.969 kb on - strand, within Pf1N1B4_5658at 88.997 kb on - strand, within Pf1N1B4_5658at 89.131 kb on - strand, within Pf1N1B4_5658at 89.586 kb on + strandat 89.807 kb on - strandat 89.809 kb on + strandat 89.809 kb on + strandat 89.809 kb on + strandat 89.809 kb on + strandat 89.809 kb on + strandat 89.809 kb on + strandat 89.810 kb on - strandat 89.810 kb on - strandat 89.810 kb on - strandat 89.810 kb on - strandat 89.810 kb on - strandat 89.812 kb on + strandat 89.813 kb on - strandat 89.871 kb on - strand, within Pf1N1B4_5659at 89.871 kb on - strand, within Pf1N1B4_5659at 89.871 kb on - strand, within Pf1N1B4_5659at 89.871 kb on - strand, within Pf1N1B4_5659at 89.871 kb on - strand, within Pf1N1B4_5659at 89.871 kb on - strand, within Pf1N1B4_5659at 89.871 kb on - strand, within Pf1N1B4_5659at 90.040 kb on + strand, within Pf1N1B4_5659at 90.040 kb on + strand, within Pf1N1B4_5659at 90.040 kb on + strand, within Pf1N1B4_5659at 90.040 kb on + strand, within Pf1N1B4_5659at 90.040 kb on + strand, within Pf1N1B4_5659at 90.041 kb on - strand, within Pf1N1B4_5659at 90.041 kb on - strand, within Pf1N1B4_5659at 90.041 kb on - strand, within Pf1N1B4_5659at 90.041 kb on - strand, within Pf1N1B4_5659at 90.041 kb on - strand, within Pf1N1B4_5659at 90.041 kb on - strand, within Pf1N1B4_5659at 90.043 kb on + strand, within Pf1N1B4_5659at 90.043 kb on + strand, within Pf1N1B4_5659at 90.043 kb on + strand, within Pf1N1B4_5659at 90.043 kb on + strand, within Pf1N1B4_5659at 90.043 kb on + strand, within Pf1N1B4_5659at 90.043 kb on + strand, within Pf1N1B4_5659at 90.043 kb on + strand, within Pf1N1B4_5659at 90.043 kb on + strand, within Pf1N1B4_5659at 90.043 kb on + strand, within Pf1N1B4_5659at 90.044 kb on - strand, within Pf1N1B4_5659at 90.044 kb on - strand, within Pf1N1B4_5659at 90.044 kb on - strand, within Pf1N1B4_5659at 90.044 kb on - strand, within Pf1N1B4_5659at 90.044 kb on - strand, within Pf1N1B4_5659at 90.044 kb on - strand, within Pf1N1B4_5659at 90.044 kb on - strand, within Pf1N1B4_5659at 90.044 kb on - strand, within Pf1N1B4_5659at 90.044 kb on - strand, within Pf1N1B4_5659at 90.044 kb on - strand, within Pf1N1B4_5659at 90.044 kb on - strand, within Pf1N1B4_5659at 90.044 kb on - strand, within Pf1N1B4_5659at 90.044 kb on - strand, within Pf1N1B4_5659at 90.044 kb on - strand, within Pf1N1B4_5659at 90.087 kb on + strand, within Pf1N1B4_5659at 90.087 kb on + strand, within Pf1N1B4_5659at 90.088 kb on - strand, within Pf1N1B4_5659at 90.088 kb on - strand, within Pf1N1B4_5659at 90.088 kb on - strand, within Pf1N1B4_5659at 90.088 kb on - strand, within Pf1N1B4_5659at 90.088 kb on - strand, within Pf1N1B4_5659at 90.142 kb on + strand, within Pf1N1B4_5659at 90.190 kb on + strand, within Pf1N1B4_5659at 90.190 kb on + strand, within Pf1N1B4_5659at 90.190 kb on + strand, within Pf1N1B4_5659at 90.190 kb on + strand, within Pf1N1B4_5659at 90.191 kb on - strand, within Pf1N1B4_5659at 90.191 kb on - strand, within Pf1N1B4_5659at 90.191 kb on - strand, within Pf1N1B4_5659at 90.191 kb on - strandat 90.191 kb on - strand, within Pf1N1B4_5659at 90.191 kb on - strand, within Pf1N1B4_5659at 90.253 kb on + strand, within Pf1N1B4_5659at 90.254 kb on - strand, within Pf1N1B4_5659at 90.274 kb on + strand, within Pf1N1B4_5659at 90.296 kb on + strand, within Pf1N1B4_5659at 90.296 kb on + strand, within Pf1N1B4_5659at 90.296 kb on + strand, within Pf1N1B4_5659at 90.296 kb on + strand, within Pf1N1B4_5659at 90.297 kb on - strand, within Pf1N1B4_5659at 90.352 kb on + strandat 90.353 kb on - strandat 90.353 kb on - strandat 90.353 kb on - strandat 90.377 kb on + strandat 90.377 kb on + strandat 90.377 kb on + strandat 90.377 kb on + strandat 90.378 kb on - strandat 90.378 kb on - strandat 90.378 kb on - strandat 90.378 kb on - strandat 90.378 kb on - strandat 90.378 kb on - strandat 90.378 kb on - strandat 90.378 kb on - strandat 90.380 kb on + strandat 90.380 kb on + strandat 90.381 kb on - strandat 90.419 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml
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87,986 + +1.3
87,986 + Pf1N1B4_5656 0.44 -0.5
88,372 - -0.1
88,884 + +0.0
88,885 - +2.5
88,968 + Pf1N1B4_5658 0.12 +1.4
88,968 + Pf1N1B4_5658 0.12 +1.5
88,968 + Pf1N1B4_5658 0.12 +1.1
88,968 + Pf1N1B4_5658 0.12 -0.1
88,968 + Pf1N1B4_5658 0.12 +0.3
88,969 - +0.9
88,969 - Pf1N1B4_5658 0.13 -0.5
88,969 - Pf1N1B4_5658 0.13 -0.1
88,969 - Pf1N1B4_5658 0.13 +0.2
88,969 - Pf1N1B4_5658 0.13 +2.3
88,997 - Pf1N1B4_5658 0.16 +1.3
89,131 - Pf1N1B4_5658 0.34 +1.6
89,586 + -0.6
89,807 - -0.5
89,809 + -2.3
89,809 + +1.1
89,809 + +0.4
89,809 + -0.5
89,809 + +0.2
89,809 + -1.1
89,810 - +0.5
89,810 - -2.3
89,810 - +0.1
89,810 - -0.1
89,810 - -0.0
89,812 + +0.0
89,813 - -0.4
89,871 - Pf1N1B4_5659 0.11 +1.1
89,871 - Pf1N1B4_5659 0.11 +0.7
89,871 - Pf1N1B4_5659 0.11 +2.3
89,871 - Pf1N1B4_5659 0.11 +1.4
89,871 - Pf1N1B4_5659 0.11 +0.5
89,871 - Pf1N1B4_5659 0.11 +0.9
89,871 - Pf1N1B4_5659 0.11 -0.5
90,040 + Pf1N1B4_5659 0.40 -0.3
90,040 + Pf1N1B4_5659 0.40 +0.3
90,040 + Pf1N1B4_5659 0.40 +1.1
90,040 + Pf1N1B4_5659 0.40 +2.1
90,040 + Pf1N1B4_5659 0.40 +0.2
90,041 - Pf1N1B4_5659 0.40 +0.6
90,041 - Pf1N1B4_5659 0.40 +1.9
90,041 - Pf1N1B4_5659 0.40 +0.7
90,041 - Pf1N1B4_5659 0.40 +0.6
90,041 - Pf1N1B4_5659 0.40 +0.5
90,041 - Pf1N1B4_5659 0.40 +0.9
90,043 + Pf1N1B4_5659 0.41 +0.9
90,043 + Pf1N1B4_5659 0.41 +1.0
90,043 + Pf1N1B4_5659 0.41 +1.5
90,043 + Pf1N1B4_5659 0.41 +0.1
90,043 + Pf1N1B4_5659 0.41 +1.0
90,043 + Pf1N1B4_5659 0.41 +0.5
90,043 + Pf1N1B4_5659 0.41 +0.6
90,043 + Pf1N1B4_5659 0.41 -0.7
90,043 + Pf1N1B4_5659 0.41 +0.7
90,044 - Pf1N1B4_5659 0.41 -0.5
90,044 - Pf1N1B4_5659 0.41 +0.3
90,044 - Pf1N1B4_5659 0.41 -0.5
90,044 - Pf1N1B4_5659 0.41 +1.1
90,044 - Pf1N1B4_5659 0.41 +0.0
90,044 - Pf1N1B4_5659 0.41 +0.4
90,044 - Pf1N1B4_5659 0.41 +1.0
90,044 - Pf1N1B4_5659 0.41 +0.7
90,044 - Pf1N1B4_5659 0.41 +0.5
90,044 - Pf1N1B4_5659 0.41 +0.5
90,044 - Pf1N1B4_5659 0.41 +0.3
90,044 - Pf1N1B4_5659 0.41 -0.1
90,044 - Pf1N1B4_5659 0.41 +1.1
90,044 - Pf1N1B4_5659 0.41 -0.2
90,087 + Pf1N1B4_5659 0.48 +1.1
90,087 + Pf1N1B4_5659 0.48 -0.8
90,088 - Pf1N1B4_5659 0.48 +2.7
90,088 - Pf1N1B4_5659 0.48 +1.4
90,088 - Pf1N1B4_5659 0.48 +2.3
90,088 - Pf1N1B4_5659 0.48 +0.2
90,088 - Pf1N1B4_5659 0.48 +1.1
90,142 + Pf1N1B4_5659 0.58 +0.8
90,190 + Pf1N1B4_5659 0.66 -0.5
90,190 + Pf1N1B4_5659 0.66 +1.1
90,190 + Pf1N1B4_5659 0.66 +1.0
90,190 + Pf1N1B4_5659 0.66 -0.3
90,191 - Pf1N1B4_5659 0.66 +0.9
90,191 - Pf1N1B4_5659 0.66 +1.2
90,191 - Pf1N1B4_5659 0.66 +0.0
90,191 - -0.5
90,191 - Pf1N1B4_5659 0.66 +1.9
90,191 - Pf1N1B4_5659 0.66 +0.1
90,253 + Pf1N1B4_5659 0.77 -0.5
90,254 - Pf1N1B4_5659 0.77 +0.5
90,274 + Pf1N1B4_5659 0.80 +0.2
90,296 + Pf1N1B4_5659 0.84 +0.7
90,296 + Pf1N1B4_5659 0.84 +1.1
90,296 + Pf1N1B4_5659 0.84 -0.5
90,296 + Pf1N1B4_5659 0.84 +1.1
90,297 - Pf1N1B4_5659 0.84 +1.4
90,352 + +2.3
90,353 - -2.5
90,353 - -0.2
90,353 - +1.0
90,377 + -2.3
90,377 + +0.4
90,377 + +1.3
90,377 + +0.8
90,378 - +0.5
90,378 - +0.6
90,378 - +0.5
90,378 - +1.1
90,378 - +1.4
90,378 - +1.1
90,378 - -0.1
90,378 - +0.5
90,380 + -0.2
90,380 + +0.9
90,381 - -0.3
90,419 + -0.6

Or see this region's nucleotide sequence