Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5655 and Pf1N1B4_5656 are separated by 11 nucleotides Pf1N1B4_5656 and Pf1N1B4_5657 overlap by 4 nucleotides Pf1N1B4_5657 and Pf1N1B4_5658 are separated by 82 nucleotides
Pf1N1B4_5655 - FIG00955561: hypothetical protein, at 87,457 to 87,663
_5655
Pf1N1B4_5656 - Predicted cobalt transporter CbtA, at 87,675 to 88,382
_5656
Pf1N1B4_5657 - Cobalt-precorrin-3b C17-methyltransferase, at 88,379 to 88,792
_5657
Pf1N1B4_5658 - Cobalt-precorrin-4 C11-methyltransferase (EC 2.1.1.133), at 88,875 to 89,621
_5658
Position (kb) 88
89 Strain fitness (log2 ratio) -1
0
1
2 at 87.581 kb on - strand at 87.787 kb on - strand, within Pf1N1B4_5656 at 87.986 kb on + strand at 87.986 kb on + strand, within Pf1N1B4_5656 at 88.372 kb on - strand at 88.884 kb on + strand at 88.885 kb on - strand at 88.968 kb on + strand, within Pf1N1B4_5658 at 88.968 kb on + strand, within Pf1N1B4_5658 at 88.968 kb on + strand, within Pf1N1B4_5658 at 88.968 kb on + strand, within Pf1N1B4_5658 at 88.968 kb on + strand, within Pf1N1B4_5658 at 88.969 kb on - strand at 88.969 kb on - strand, within Pf1N1B4_5658 at 88.969 kb on - strand, within Pf1N1B4_5658 at 88.969 kb on - strand, within Pf1N1B4_5658 at 88.969 kb on - strand, within Pf1N1B4_5658 at 88.997 kb on - strand, within Pf1N1B4_5658 at 89.131 kb on - strand, within Pf1N1B4_5658 at 89.586 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 87,581 - -0.1 87,787 - Pf1N1B4_5656 0.16 +0.1 87,986 + +1.3 87,986 + Pf1N1B4_5656 0.44 -0.5 88,372 - -0.1 88,884 + +0.0 88,885 - +2.5 88,968 + Pf1N1B4_5658 0.12 +1.4 88,968 + Pf1N1B4_5658 0.12 +1.5 88,968 + Pf1N1B4_5658 0.12 +1.1 88,968 + Pf1N1B4_5658 0.12 -0.1 88,968 + Pf1N1B4_5658 0.12 +0.3 88,969 - +0.9 88,969 - Pf1N1B4_5658 0.13 -0.5 88,969 - Pf1N1B4_5658 0.13 -0.1 88,969 - Pf1N1B4_5658 0.13 +0.2 88,969 - Pf1N1B4_5658 0.13 +2.3 88,997 - Pf1N1B4_5658 0.16 +1.3 89,131 - Pf1N1B4_5658 0.34 +1.6 89,586 + -0.6
Or see this region's nucleotide sequence