Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5654 and Pf1N1B4_5655 are separated by 927 nucleotides Pf1N1B4_5655 and Pf1N1B4_5656 are separated by 11 nucleotides Pf1N1B4_5656 and Pf1N1B4_5657 overlap by 4 nucleotides
Pf1N1B4_5654 - CobW GTPase involved in cobalt insertion for B12 biosynthesis, at 85,468 to 86,529
_5654
Pf1N1B4_5655 - FIG00955561: hypothetical protein, at 87,457 to 87,663
_5655
Pf1N1B4_5656 - Predicted cobalt transporter CbtA, at 87,675 to 88,382
_5656
Pf1N1B4_5657 - Cobalt-precorrin-3b C17-methyltransferase, at 88,379 to 88,792
_5657
Position (kb) 87
88 Strain fitness (log2 ratio) -2
-1
0
1 at 86.802 kb on + strand at 86.802 kb on + strand at 86.802 kb on + strand at 86.803 kb on - strand at 86.803 kb on - strand at 86.844 kb on - strand at 87.110 kb on + strand at 87.111 kb on - strand at 87.111 kb on - strand at 87.111 kb on - strand at 87.178 kb on - strand at 87.249 kb on + strand at 87.250 kb on - strand at 87.581 kb on - strand at 87.787 kb on - strand, within Pf1N1B4_5656 at 87.986 kb on + strand at 87.986 kb on + strand, within Pf1N1B4_5656 at 88.372 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 86,802 + +1.3 86,802 + -0.3 86,802 + +0.5 86,803 - -0.9 86,803 - -2.1 86,844 - -0.4 87,110 + +0.3 87,111 - +0.8 87,111 - -0.5 87,111 - -2.1 87,178 - -0.2 87,249 + +0.3 87,250 - +1.5 87,581 - -0.1 87,787 - Pf1N1B4_5656 0.16 +0.1 87,986 + +1.3 87,986 + Pf1N1B4_5656 0.44 -0.5 88,372 - -0.1
Or see this region's nucleotide sequence