Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5614 and Pf1N1B4_5615 overlap by 4 nucleotides Pf1N1B4_5615 and Pf1N1B4_5616 are separated by 61 nucleotides Pf1N1B4_5616 and Pf1N1B4_6052 are separated by 168 nucleotides Pf1N1B4_6052 and Pf1N1B4_5617 are separated by 63 nucleotides Pf1N1B4_5617 and Pf1N1B4_5618 are separated by 33 nucleotides
Pf1N1B4_5614 - COGs COG3777, at 40,025 to 40,867
_5614
Pf1N1B4_5615 - Citrate lyase beta chain (EC 4.1.3.6), at 40,864 to 41,679
_5615
Pf1N1B4_5616 - Succinate-semialdehyde dehydrogenase [NAD] (EC 1.2.1.24); Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 41,741 to 43,132
_5616
Pf1N1B4_6052 - tRNA-Gly-GCC, at 43,301 to 43,373
_6052
Pf1N1B4_5617 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (EC 2.7.8.5), at 43,437 to 43,997
_5617
Pf1N1B4_5618 - Excinuclease ABC subunit C, at 44,031 to 45,854
_5618
Position (kb) 41
42
43
44 Strain fitness (log2 ratio) -1
0
1
2
3 at 41.433 kb on + strand, within Pf1N1B4_5615 at 41.682 kb on - strand at 41.807 kb on + strand at 41.931 kb on + strand, within Pf1N1B4_5616 at 42.212 kb on + strand, within Pf1N1B4_5616 at 42.212 kb on + strand, within Pf1N1B4_5616 at 42.212 kb on + strand, within Pf1N1B4_5616 at 42.213 kb on - strand, within Pf1N1B4_5616 at 42.213 kb on - strand, within Pf1N1B4_5616 at 42.251 kb on + strand, within Pf1N1B4_5616 at 42.659 kb on + strand, within Pf1N1B4_5616 at 42.659 kb on + strand at 42.659 kb on + strand, within Pf1N1B4_5616 at 42.660 kb on - strand, within Pf1N1B4_5616 at 42.660 kb on - strand, within Pf1N1B4_5616 at 42.687 kb on + strand, within Pf1N1B4_5616 at 42.936 kb on - strand, within Pf1N1B4_5616 at 43.439 kb on - strand at 44.033 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 41,433 + Pf1N1B4_5615 0.70 -0.1 41,682 - +2.9 41,807 + -1.5 41,931 + Pf1N1B4_5616 0.14 +2.5 42,212 + Pf1N1B4_5616 0.34 -1.1 42,212 + Pf1N1B4_5616 0.34 +2.3 42,212 + Pf1N1B4_5616 0.34 -0.1 42,213 - Pf1N1B4_5616 0.34 -0.1 42,213 - Pf1N1B4_5616 0.34 +0.5 42,251 + Pf1N1B4_5616 0.37 -1.6 42,659 + Pf1N1B4_5616 0.66 -0.5 42,659 + -0.8 42,659 + Pf1N1B4_5616 0.66 +0.4 42,660 - Pf1N1B4_5616 0.66 -0.1 42,660 - Pf1N1B4_5616 0.66 +0.9 42,687 + Pf1N1B4_5616 0.68 +0.5 42,936 - Pf1N1B4_5616 0.86 +0.3 43,439 - +0.5 44,033 - +0.4
Or see this region's nucleotide sequence