Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5599 and Pf1N1B4_5600 are separated by 91 nucleotides Pf1N1B4_5600 and Pf1N1B4_5601 are separated by 39 nucleotides
Pf1N1B4_5599 - transcriptional regulator, TetR family, at 21,783 to 22,394
_5599
Pf1N1B4_5600 - Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2), at 22,486 to 23,589
_5600
Pf1N1B4_5601 - FIG00966546: hypothetical protein, at 23,629 to 24,966
_5601
Position (kb)
22
23
24 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 21.621 kb on + strand at 21.934 kb on - strand at 22.063 kb on + strand, within Pf1N1B4_5599 at 22.064 kb on - strand, within Pf1N1B4_5599 at 22.351 kb on + strand at 22.359 kb on - strand at 22.377 kb on + strand at 22.592 kb on + strand at 22.592 kb on + strand at 22.592 kb on + strand at 22.592 kb on + strand at 22.592 kb on + strand at 22.592 kb on + strand at 22.593 kb on - strand at 22.593 kb on - strand at 22.593 kb on - strand at 22.593 kb on - strand at 22.593 kb on - strand at 22.593 kb on - strand at 22.593 kb on - strand at 22.593 kb on - strand at 22.593 kb on - strand at 22.606 kb on - strand, within Pf1N1B4_5600 at 22.707 kb on - strand, within Pf1N1B4_5600 at 22.707 kb on - strand, within Pf1N1B4_5600 at 22.707 kb on - strand, within Pf1N1B4_5600 at 22.721 kb on + strand, within Pf1N1B4_5600 at 22.721 kb on + strand, within Pf1N1B4_5600 at 22.721 kb on + strand, within Pf1N1B4_5600 at 22.722 kb on - strand, within Pf1N1B4_5600 at 22.932 kb on - strand, within Pf1N1B4_5600 at 22.932 kb on - strand, within Pf1N1B4_5600 at 23.018 kb on + strand, within Pf1N1B4_5600 at 23.019 kb on - strand at 23.019 kb on - strand, within Pf1N1B4_5600 at 23.019 kb on - strand, within Pf1N1B4_5600 at 23.019 kb on - strand, within Pf1N1B4_5600 at 23.199 kb on - strand at 23.210 kb on + strand, within Pf1N1B4_5600 at 23.424 kb on - strand, within Pf1N1B4_5600 at 23.424 kb on - strand, within Pf1N1B4_5600 at 23.551 kb on + strand at 23.552 kb on - strand at 23.571 kb on + strand at 23.630 kb on + strand at 23.634 kb on - strand at 23.648 kb on + strand at 23.648 kb on + strand at 23.649 kb on - strand at 23.649 kb on - strand at 23.724 kb on - strand at 23.736 kb on + strand at 23.739 kb on - strand at 23.739 kb on - strand at 23.819 kb on + strand, within Pf1N1B4_5601 at 23.846 kb on + strand, within Pf1N1B4_5601 at 23.933 kb on + strand, within Pf1N1B4_5601 at 23.933 kb on + strand at 24.128 kb on + strand, within Pf1N1B4_5601 at 24.227 kb on + strand, within Pf1N1B4_5601 at 24.227 kb on + strand, within Pf1N1B4_5601 at 24.228 kb on - strand, within Pf1N1B4_5601 at 24.333 kb on - strand, within Pf1N1B4_5601 at 24.533 kb on + strand, within Pf1N1B4_5601 at 24.533 kb on + strand, within Pf1N1B4_5601 at 24.534 kb on - strand, within Pf1N1B4_5601 at 24.584 kb on + strand, within Pf1N1B4_5601 at 24.585 kb on - strand, within Pf1N1B4_5601
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 21,621 + -0.4 21,934 - -0.1 22,063 + Pf1N1B4_5599 0.46 +0.8 22,064 - Pf1N1B4_5599 0.46 +1.4 22,351 + +0.5 22,359 - -0.2 22,377 + -0.2 22,592 + -0.5 22,592 + -0.3 22,592 + +1.9 22,592 + -1.3 22,592 + -2.2 22,592 + +1.1 22,593 - -0.3 22,593 - -0.8 22,593 - -1.8 22,593 - -0.8 22,593 - +0.0 22,593 - +0.8 22,593 - -0.6 22,593 - +1.3 22,593 - -0.2 22,606 - Pf1N1B4_5600 0.11 -0.7 22,707 - Pf1N1B4_5600 0.20 -1.5 22,707 - Pf1N1B4_5600 0.20 +0.3 22,707 - Pf1N1B4_5600 0.20 -1.8 22,721 + Pf1N1B4_5600 0.21 -0.7 22,721 + Pf1N1B4_5600 0.21 +0.2 22,721 + Pf1N1B4_5600 0.21 +1.5 22,722 - Pf1N1B4_5600 0.21 +0.5 22,932 - Pf1N1B4_5600 0.40 +0.5 22,932 - Pf1N1B4_5600 0.40 +1.0 23,018 + Pf1N1B4_5600 0.48 -1.5 23,019 - +0.9 23,019 - Pf1N1B4_5600 0.48 +1.6 23,019 - Pf1N1B4_5600 0.48 -0.5 23,019 - Pf1N1B4_5600 0.48 -2.1 23,199 - -0.5 23,210 + Pf1N1B4_5600 0.66 -2.1 23,424 - Pf1N1B4_5600 0.85 +0.5 23,424 - Pf1N1B4_5600 0.85 -1.2 23,551 + -0.3 23,552 - -0.9 23,571 + -1.8 23,630 + +0.5 23,634 - -0.1 23,648 + +0.8 23,648 + -2.3 23,649 - -1.6 23,649 - +0.5 23,724 - +0.2 23,736 + +0.5 23,739 - +0.8 23,739 - -0.5 23,819 + Pf1N1B4_5601 0.14 -1.3 23,846 + Pf1N1B4_5601 0.16 -1.3 23,933 + Pf1N1B4_5601 0.23 +0.3 23,933 + -0.5 24,128 + Pf1N1B4_5601 0.37 +0.2 24,227 + Pf1N1B4_5601 0.45 +0.3 24,227 + Pf1N1B4_5601 0.45 +0.2 24,228 - Pf1N1B4_5601 0.45 -2.3 24,333 - Pf1N1B4_5601 0.53 -0.5 24,533 + Pf1N1B4_5601 0.68 +0.5 24,533 + Pf1N1B4_5601 0.68 -2.6 24,534 - Pf1N1B4_5601 0.68 +1.1 24,584 + Pf1N1B4_5601 0.71 +0.8 24,585 - Pf1N1B4_5601 0.71 -0.5
Or see this region's nucleotide sequence