Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5462 and Pf1N1B4_5463 are separated by 281 nucleotides Pf1N1B4_5463 and Pf1N1B4_5464 are separated by 325 nucleotides Pf1N1B4_5464 and Pf1N1B4_5465 are separated by 7 nucleotides
Pf1N1B4_5462 - hypothetical protein, at 209,255 to 210,247
_5462
Pf1N1B4_5463 - hypothetical protein, at 210,529 to 210,654
_5463
Pf1N1B4_5464 - Cointegrate resolution protein T, at 210,980 to 212,032
_5464
Pf1N1B4_5465 - hypothetical protein, at 212,040 to 213,158
_5465
Position (kb) 210
211
212
213 Strain fitness (log2 ratio) -2
-1
0
1
2 at 209.996 kb on + strand, within Pf1N1B4_5462 at 209.996 kb on + strand at 209.996 kb on + strand, within Pf1N1B4_5462 at 209.997 kb on - strand, within Pf1N1B4_5462 at 209.997 kb on - strand, within Pf1N1B4_5462 at 210.000 kb on + strand, within Pf1N1B4_5462 at 210.000 kb on + strand, within Pf1N1B4_5462 at 210.000 kb on + strand, within Pf1N1B4_5462 at 210.000 kb on + strand, within Pf1N1B4_5462 at 210.000 kb on + strand at 210.000 kb on + strand, within Pf1N1B4_5462 at 210.000 kb on + strand, within Pf1N1B4_5462 at 210.001 kb on - strand, within Pf1N1B4_5462 at 210.001 kb on - strand, within Pf1N1B4_5462 at 210.001 kb on - strand, within Pf1N1B4_5462 at 210.001 kb on - strand, within Pf1N1B4_5462 at 210.004 kb on + strand, within Pf1N1B4_5462 at 210.041 kb on + strand, within Pf1N1B4_5462 at 210.041 kb on + strand, within Pf1N1B4_5462 at 210.041 kb on + strand, within Pf1N1B4_5462 at 210.041 kb on + strand, within Pf1N1B4_5462 at 210.041 kb on + strand, within Pf1N1B4_5462 at 210.042 kb on - strand, within Pf1N1B4_5462 at 210.042 kb on - strand, within Pf1N1B4_5462 at 210.042 kb on - strand, within Pf1N1B4_5462 at 210.042 kb on - strand, within Pf1N1B4_5462 at 210.078 kb on + strand, within Pf1N1B4_5462 at 210.078 kb on + strand, within Pf1N1B4_5462 at 210.078 kb on + strand, within Pf1N1B4_5462 at 210.078 kb on + strand, within Pf1N1B4_5462 at 210.079 kb on - strand, within Pf1N1B4_5462 at 210.079 kb on - strand at 210.133 kb on + strand, within Pf1N1B4_5462 at 210.136 kb on + strand, within Pf1N1B4_5462 at 210.136 kb on + strand, within Pf1N1B4_5462 at 210.137 kb on - strand, within Pf1N1B4_5462 at 210.141 kb on + strand, within Pf1N1B4_5462 at 210.146 kb on + strand, within Pf1N1B4_5462 at 210.146 kb on + strand at 210.228 kb on + strand at 210.229 kb on - strand at 210.243 kb on + strand at 210.245 kb on + strand at 210.245 kb on + strand at 210.245 kb on + strand at 210.250 kb on + strand at 210.250 kb on + strand at 210.250 kb on + strand at 210.261 kb on + strand at 210.303 kb on - strand at 210.313 kb on - strand at 210.313 kb on - strand at 210.313 kb on - strand at 210.315 kb on + strand at 210.316 kb on - strand at 210.316 kb on - strand at 210.330 kb on - strand at 210.343 kb on + strand at 210.343 kb on + strand at 210.343 kb on + strand at 210.343 kb on + strand at 210.343 kb on + strand at 210.344 kb on - strand at 210.344 kb on - strand at 210.344 kb on - strand at 210.344 kb on - strand at 210.344 kb on - strand at 210.344 kb on - strand at 210.344 kb on - strand at 210.344 kb on - strand at 210.344 kb on - strand at 210.408 kb on - strand at 210.408 kb on - strand at 210.408 kb on - strand at 210.435 kb on + strand at 210.436 kb on - strand at 210.552 kb on - strand, within Pf1N1B4_5463 at 210.557 kb on + strand at 210.566 kb on - strand, within Pf1N1B4_5463 at 210.566 kb on - strand, within Pf1N1B4_5463 at 210.566 kb on - strand, within Pf1N1B4_5463 at 210.566 kb on - strand, within Pf1N1B4_5463 at 210.566 kb on - strand, within Pf1N1B4_5463 at 210.690 kb on + strand at 210.759 kb on - strand at 210.760 kb on + strand at 210.761 kb on - strand at 210.761 kb on - strand at 210.761 kb on - strand at 210.790 kb on - strand at 210.790 kb on - strand at 210.790 kb on - strand at 210.790 kb on - strand at 210.790 kb on - strand at 210.822 kb on + strand at 210.823 kb on - strand at 210.826 kb on + strand at 210.826 kb on + strand at 210.827 kb on - strand at 211.342 kb on - strand, within Pf1N1B4_5464 at 211.342 kb on - strand, within Pf1N1B4_5464 at 211.354 kb on - strand, within Pf1N1B4_5464 at 211.373 kb on + strand, within Pf1N1B4_5464 at 211.458 kb on + strand, within Pf1N1B4_5464 at 211.458 kb on + strand, within Pf1N1B4_5464 at 211.809 kb on + strand, within Pf1N1B4_5464 at 211.809 kb on + strand, within Pf1N1B4_5464 at 211.943 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 209,996 + Pf1N1B4_5462 0.75 -2.1 209,996 + -0.7 209,996 + Pf1N1B4_5462 0.75 -0.5 209,997 - Pf1N1B4_5462 0.75 +0.3 209,997 - Pf1N1B4_5462 0.75 -0.8 210,000 + Pf1N1B4_5462 0.75 +1.0 210,000 + Pf1N1B4_5462 0.75 -0.8 210,000 + Pf1N1B4_5462 0.75 +1.1 210,000 + Pf1N1B4_5462 0.75 +0.1 210,000 + +0.9 210,000 + Pf1N1B4_5462 0.75 +1.0 210,000 + Pf1N1B4_5462 0.75 +0.3 210,001 - Pf1N1B4_5462 0.75 +0.3 210,001 - Pf1N1B4_5462 0.75 -1.7 210,001 - Pf1N1B4_5462 0.75 -0.7 210,001 - Pf1N1B4_5462 0.75 +0.8 210,004 + Pf1N1B4_5462 0.75 +0.6 210,041 + Pf1N1B4_5462 0.79 +1.6 210,041 + Pf1N1B4_5462 0.79 -0.7 210,041 + Pf1N1B4_5462 0.79 -0.1 210,041 + Pf1N1B4_5462 0.79 -0.5 210,041 + Pf1N1B4_5462 0.79 -1.7 210,042 - Pf1N1B4_5462 0.79 +0.0 210,042 - Pf1N1B4_5462 0.79 -0.1 210,042 - Pf1N1B4_5462 0.79 +2.3 210,042 - Pf1N1B4_5462 0.79 -0.1 210,078 + Pf1N1B4_5462 0.83 -0.1 210,078 + Pf1N1B4_5462 0.83 -2.0 210,078 + Pf1N1B4_5462 0.83 -0.3 210,078 + Pf1N1B4_5462 0.83 -2.1 210,079 - Pf1N1B4_5462 0.83 -0.3 210,079 - -0.1 210,133 + Pf1N1B4_5462 0.88 -0.9 210,136 + Pf1N1B4_5462 0.89 -0.1 210,136 + Pf1N1B4_5462 0.89 -0.7 210,137 - Pf1N1B4_5462 0.89 -0.7 210,141 + Pf1N1B4_5462 0.89 -1.1 210,146 + Pf1N1B4_5462 0.90 -0.1 210,146 + +1.4 210,228 + +1.4 210,229 - -1.7 210,243 + +0.9 210,245 + -0.1 210,245 + -0.0 210,245 + +0.3 210,250 + -0.4 210,250 + -0.7 210,250 + +0.4 210,261 + +1.9 210,303 - +0.3 210,313 - -0.7 210,313 - -0.4 210,313 - +0.5 210,315 + +0.3 210,316 - -1.7 210,316 - -1.5 210,330 - -0.8 210,343 + -1.7 210,343 + -0.1 210,343 + +1.9 210,343 + -0.7 210,343 + -1.7 210,344 - -2.3 210,344 - +0.9 210,344 - +0.0 210,344 - +0.6 210,344 - +1.0 210,344 - +1.7 210,344 - -0.0 210,344 - +1.2 210,344 - -0.7 210,408 - -2.0 210,408 - -1.3 210,408 - -1.0 210,435 + -1.5 210,436 - +1.2 210,552 - Pf1N1B4_5463 0.18 -1.1 210,557 + +0.9 210,566 - Pf1N1B4_5463 0.29 +1.1 210,566 - Pf1N1B4_5463 0.29 -0.6 210,566 - Pf1N1B4_5463 0.29 -1.9 210,566 - Pf1N1B4_5463 0.29 -1.7 210,566 - Pf1N1B4_5463 0.29 -1.1 210,690 + +0.3 210,759 - +1.4 210,760 + -0.3 210,761 - +1.0 210,761 - -0.1 210,761 - +0.1 210,790 - +0.4 210,790 - +0.9 210,790 - -0.7 210,790 - +1.1 210,790 - +0.1 210,822 + +1.2 210,823 - +0.9 210,826 + +0.9 210,826 + -0.7 210,827 - +1.5 211,342 - Pf1N1B4_5464 0.34 +0.9 211,342 - Pf1N1B4_5464 0.34 -1.5 211,354 - Pf1N1B4_5464 0.36 -1.1 211,373 + Pf1N1B4_5464 0.37 -1.7 211,458 + Pf1N1B4_5464 0.45 +0.7 211,458 + Pf1N1B4_5464 0.45 -2.0 211,809 + Pf1N1B4_5464 0.79 -0.1 211,809 + Pf1N1B4_5464 0.79 -1.1 211,943 - +0.1
Or see this region's nucleotide sequence