Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5446 and Pf1N1B4_5447 are separated by 46 nucleotides
Pf1N1B4_5446 - hypothetical protein, at 192,136 to 192,315
_5446
Pf1N1B4_5447 - hypothetical protein, at 192,362 to 192,556
_5447
Position (kb)
192
193 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 191.139 kb on - strand at 191.139 kb on - strand at 191.139 kb on - strand at 191.139 kb on - strand at 191.139 kb on - strand at 191.139 kb on - strand at 191.193 kb on - strand at 191.193 kb on - strand at 191.193 kb on - strand at 191.193 kb on - strand at 191.193 kb on - strand at 191.193 kb on - strand at 191.193 kb on - strand at 191.225 kb on - strand at 191.237 kb on - strand at 191.283 kb on - strand at 191.289 kb on + strand at 191.289 kb on + strand at 191.289 kb on + strand at 191.290 kb on - strand at 191.290 kb on - strand at 191.290 kb on - strand at 191.290 kb on - strand at 191.290 kb on - strand at 191.293 kb on + strand at 191.293 kb on + strand at 191.294 kb on - strand at 191.294 kb on - strand at 191.294 kb on - strand at 191.294 kb on - strand at 191.335 kb on + strand at 191.335 kb on + strand at 191.335 kb on + strand at 191.336 kb on - strand at 191.336 kb on - strand at 191.336 kb on - strand at 191.339 kb on + strand at 191.339 kb on + strand at 191.340 kb on - strand at 191.340 kb on - strand at 191.340 kb on - strand at 191.340 kb on - strand at 191.340 kb on - strand at 191.340 kb on - strand at 191.430 kb on + strand at 191.432 kb on + strand at 191.433 kb on - strand at 191.478 kb on + strand at 191.537 kb on + strand at 191.537 kb on + strand at 191.537 kb on + strand at 191.537 kb on + strand at 191.537 kb on + strand at 191.537 kb on + strand at 191.537 kb on + strand at 191.538 kb on - strand at 191.538 kb on - strand at 191.538 kb on - strand at 191.538 kb on - strand at 191.538 kb on - strand at 191.538 kb on - strand at 191.541 kb on + strand at 191.542 kb on - strand at 191.576 kb on - strand at 191.576 kb on - strand at 191.603 kb on - strand at 191.615 kb on + strand at 191.616 kb on - strand at 191.628 kb on + strand at 191.628 kb on + strand at 191.628 kb on + strand at 191.628 kb on + strand at 191.628 kb on + strand at 191.628 kb on + strand at 191.628 kb on + strand at 191.629 kb on - strand at 191.629 kb on - strand at 191.629 kb on - strand at 191.629 kb on - strand at 191.632 kb on + strand at 191.697 kb on + strand at 191.697 kb on + strand at 191.698 kb on - strand at 191.716 kb on + strand at 191.717 kb on - strand at 191.717 kb on - strand at 191.717 kb on - strand at 191.719 kb on + strand at 191.720 kb on - strand at 191.720 kb on - strand at 191.720 kb on - strand at 191.720 kb on - strand at 191.720 kb on - strand at 191.737 kb on + strand at 191.738 kb on - strand at 191.781 kb on - strand at 191.802 kb on - strand at 191.802 kb on - strand at 191.802 kb on - strand at 191.815 kb on - strand at 191.815 kb on - strand at 191.815 kb on - strand at 191.836 kb on + strand at 191.837 kb on - strand at 191.837 kb on - strand at 191.851 kb on + strand at 191.852 kb on - strand at 191.881 kb on - strand at 191.892 kb on + strand at 191.893 kb on - strand at 191.893 kb on - strand at 191.893 kb on - strand at 191.906 kb on + strand at 191.906 kb on + strand at 191.907 kb on - strand at 191.907 kb on - strand at 191.907 kb on - strand at 191.907 kb on - strand at 191.927 kb on + strand at 191.927 kb on + strand at 191.927 kb on + strand at 191.927 kb on + strand at 191.927 kb on + strand at 191.927 kb on + strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 192.086 kb on - strand at 192.086 kb on - strand at 192.086 kb on - strand at 192.129 kb on - strand at 192.137 kb on + strand at 192.137 kb on + strand at 192.172 kb on - strand, within Pf1N1B4_5446 at 192.229 kb on + strand, within Pf1N1B4_5446 at 192.229 kb on + strand, within Pf1N1B4_5446 at 192.230 kb on - strand, within Pf1N1B4_5446 at 192.230 kb on - strand, within Pf1N1B4_5446 at 192.230 kb on - strand, within Pf1N1B4_5446 at 192.249 kb on - strand, within Pf1N1B4_5446 at 192.387 kb on + strand, within Pf1N1B4_5447 at 192.388 kb on - strand, within Pf1N1B4_5447 at 192.388 kb on - strand, within Pf1N1B4_5447 at 192.388 kb on - strand, within Pf1N1B4_5447 at 192.388 kb on - strand, within Pf1N1B4_5447 at 192.412 kb on + strand, within Pf1N1B4_5447 at 192.441 kb on - strand at 192.441 kb on - strand, within Pf1N1B4_5447 at 192.441 kb on - strand at 192.540 kb on + strand at 192.540 kb on + strand at 192.541 kb on - strand at 192.541 kb on - strand at 192.541 kb on - strand at 192.541 kb on - strand at 192.541 kb on - strand at 192.551 kb on + strand at 192.552 kb on - strand at 192.552 kb on - strand at 192.571 kb on - strand at 192.573 kb on + strand at 192.574 kb on - strand at 192.574 kb on - strand at 192.635 kb on - strand at 192.671 kb on - strand at 192.671 kb on - strand at 192.671 kb on - strand at 192.677 kb on + strand at 192.677 kb on + strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.733 kb on - strand at 192.733 kb on - strand at 192.733 kb on - strand at 192.733 kb on - strand at 192.740 kb on + strand at 192.741 kb on - strand at 192.741 kb on - strand at 192.741 kb on - strand at 192.741 kb on - strand at 192.741 kb on - strand at 192.761 kb on + strand at 192.761 kb on + strand at 192.761 kb on + strand at 192.761 kb on + strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.853 kb on - strand at 192.853 kb on - strand at 192.853 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.934 kb on - strand at 192.934 kb on - strand at 192.945 kb on - strand at 192.945 kb on - strand at 192.945 kb on - strand at 193.010 kb on - strand at 193.066 kb on - strand at 193.066 kb on - strand at 193.123 kb on + strand at 193.146 kb on + strand at 193.147 kb on - strand at 193.147 kb on - strand at 193.170 kb on - strand at 193.170 kb on - strand at 193.208 kb on + strand at 193.209 kb on - strand at 193.209 kb on - strand at 193.267 kb on - strand at 193.267 kb on - strand at 193.267 kb on - strand at 193.267 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 191,139 - +0.9 191,139 - -1.0 191,139 - -0.7 191,139 - +0.0 191,139 - +0.7 191,139 - -1.7 191,193 - -1.5 191,193 - -0.7 191,193 - -2.0 191,193 - +0.3 191,193 - +0.0 191,193 - +0.2 191,193 - +1.2 191,225 - -0.9 191,237 - -0.1 191,283 - +1.3 191,289 + -0.5 191,289 + +0.4 191,289 + -0.0 191,290 - -0.7 191,290 - +0.5 191,290 - -2.8 191,290 - -0.1 191,290 - +0.3 191,293 + +2.4 191,293 + -0.7 191,294 - +0.4 191,294 - +0.1 191,294 - -0.7 191,294 - +0.6 191,335 + +0.8 191,335 + -0.4 191,335 + -0.4 191,336 - -0.1 191,336 - -0.4 191,336 - -2.0 191,339 + -2.0 191,339 + +1.9 191,340 - +1.2 191,340 - +0.2 191,340 - +1.4 191,340 - +2.2 191,340 - -0.7 191,340 - +0.9 191,430 + +2.4 191,432 + -2.0 191,433 - -0.4 191,478 + +1.5 191,537 + +0.9 191,537 + -2.0 191,537 + +0.0 191,537 + -0.8 191,537 + -0.1 191,537 + +1.2 191,537 + +0.1 191,538 - +0.9 191,538 - +0.9 191,538 - +0.6 191,538 - +0.4 191,538 - +0.2 191,538 - +0.1 191,541 + +0.9 191,542 - +0.0 191,576 - -0.1 191,576 - +0.0 191,603 - -0.1 191,615 + -2.0 191,616 - +0.4 191,628 + -1.1 191,628 + -0.7 191,628 + +1.3 191,628 + -0.6 191,628 + +0.0 191,628 + -2.1 191,628 + -1.0 191,629 - +1.7 191,629 - -0.5 191,629 - +0.3 191,629 - +2.4 191,632 + -0.1 191,697 + +0.5 191,697 + -3.0 191,698 - -0.8 191,716 + -1.6 191,717 - +0.5 191,717 - +2.1 191,717 - -0.4 191,719 + -2.0 191,720 - +0.4 191,720 - -1.7 191,720 - +1.1 191,720 - -0.7 191,720 - +1.7 191,737 + +0.6 191,738 - +0.0 191,781 - -0.5 191,802 - -0.3 191,802 - +0.9 191,802 - +0.3 191,815 - +2.1 191,815 - +0.5 191,815 - +0.7 191,836 + -1.5 191,837 - -2.0 191,837 - -1.3 191,851 + +0.9 191,852 - -0.3 191,881 - +0.1 191,892 + +0.4 191,893 - -1.7 191,893 - +1.4 191,893 - -1.1 191,906 + -0.1 191,906 + -0.1 191,907 - -0.6 191,907 - +0.9 191,907 - +0.6 191,907 - -0.3 191,927 + -0.6 191,927 + +0.4 191,927 + +0.6 191,927 + +1.0 191,927 + -1.0 191,927 + -0.1 191,928 - -0.5 191,928 - +0.7 191,928 - -0.5 191,928 - -0.7 191,928 - -0.7 191,928 - +0.9 191,928 - -0.7 191,928 - -1.0 191,928 - -0.7 191,928 - -2.3 191,928 - -2.3 191,928 - +0.3 192,086 - +1.1 192,086 - -1.3 192,086 - +0.7 192,129 - -0.3 192,137 + +0.5 192,137 + +0.9 192,172 - Pf1N1B4_5446 0.20 -2.6 192,229 + Pf1N1B4_5446 0.52 -2.6 192,229 + Pf1N1B4_5446 0.52 -0.7 192,230 - Pf1N1B4_5446 0.52 -1.6 192,230 - Pf1N1B4_5446 0.52 +0.4 192,230 - Pf1N1B4_5446 0.52 -0.6 192,249 - Pf1N1B4_5446 0.63 -0.3 192,387 + Pf1N1B4_5447 0.13 +0.0 192,388 - Pf1N1B4_5447 0.13 +1.0 192,388 - Pf1N1B4_5447 0.13 -0.4 192,388 - Pf1N1B4_5447 0.13 +0.4 192,388 - Pf1N1B4_5447 0.13 -0.1 192,412 + Pf1N1B4_5447 0.26 +0.3 192,441 - +0.9 192,441 - Pf1N1B4_5447 0.41 -0.2 192,441 - +0.9 192,540 + -1.0 192,540 + -0.1 192,541 - -0.1 192,541 - +0.4 192,541 - +0.5 192,541 - +0.9 192,541 - +0.3 192,551 + -0.1 192,552 - -0.5 192,552 - +0.3 192,571 - +1.2 192,573 + -0.6 192,574 - -0.7 192,574 - +0.3 192,635 - +0.1 192,671 - +1.1 192,671 - -1.5 192,671 - +0.3 192,677 + -2.1 192,677 + -0.0 192,678 - +1.3 192,678 - +0.6 192,678 - +0.4 192,678 - -0.1 192,678 - +1.5 192,678 - -0.2 192,678 - -2.1 192,678 - +0.6 192,678 - +0.0 192,678 - +0.1 192,678 - -2.0 192,678 - +0.2 192,678 - -2.0 192,678 - +0.0 192,678 - +1.6 192,678 - +0.9 192,678 - +1.2 192,678 - -0.8 192,678 - +0.9 192,678 - +0.2 192,733 - +0.5 192,733 - -0.2 192,733 - +0.7 192,733 - -0.2 192,740 + +0.2 192,741 - -0.5 192,741 - +0.4 192,741 - -2.2 192,741 - -1.0 192,741 - -0.8 192,761 + -1.0 192,761 + +3.0 192,761 + +1.4 192,761 + -1.3 192,762 - +1.2 192,762 - +1.1 192,762 - -0.7 192,762 - +1.0 192,762 - +1.3 192,762 - -1.0 192,762 - -0.4 192,762 - +0.1 192,762 - +1.7 192,762 - -0.8 192,762 - -0.5 192,762 - +0.6 192,762 - +0.1 192,762 - +1.5 192,762 - -0.4 192,762 - +0.5 192,762 - -0.6 192,762 - -0.9 192,762 - +0.4 192,762 - -0.0 192,762 - -0.1 192,853 - -0.7 192,853 - +0.3 192,853 - +0.8 192,863 - -0.8 192,863 - +0.8 192,863 - +0.7 192,863 - -0.4 192,863 - +0.9 192,863 - -0.7 192,863 - -0.2 192,863 - -0.5 192,863 - +0.4 192,863 - -0.8 192,863 - -1.1 192,934 - +1.1 192,934 - -0.1 192,945 - -0.1 192,945 - +0.3 192,945 - -0.1 193,010 - +0.9 193,066 - +2.2 193,066 - +0.6 193,123 + +0.2 193,146 + +0.6 193,147 - +0.2 193,147 - +2.4 193,170 - +0.5 193,170 - -0.4 193,208 + -0.1 193,209 - +1.2 193,209 - +0.3 193,267 - +0.5 193,267 - +0.8 193,267 - +0.7 193,267 - -0.1
Or see this region's nucleotide sequence