Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_543 and Pf1N1B4_544 are separated by 193 nucleotides Pf1N1B4_544 and Pf1N1B4_545 are separated by 105 nucleotides
Pf1N1B4_543 - ATP-dependent RNA helicase SrmB, at 639,950 to 641,296
_543
Pf1N1B4_544 - Small-conductance mechanosensitive channel, at 641,490 to 642,788
_544
Pf1N1B4_545 - MadN protein, at 642,894 to 643,760
_545
Position (kb) 641
642
643 Strain fitness (log2 ratio) -2
-1
0
1 at 641.317 kb on - strand at 641.335 kb on - strand at 641.372 kb on - strand at 641.373 kb on + strand at 641.373 kb on + strand at 641.440 kb on + strand at 641.440 kb on + strand at 641.440 kb on + strand at 641.441 kb on - strand at 641.441 kb on - strand at 641.441 kb on - strand at 641.602 kb on + strand at 641.603 kb on - strand at 641.786 kb on + strand, within Pf1N1B4_544 at 641.874 kb on + strand, within Pf1N1B4_544 at 642.378 kb on + strand, within Pf1N1B4_544 at 642.501 kb on + strand at 642.502 kb on - strand, within Pf1N1B4_544 at 642.591 kb on + strand, within Pf1N1B4_544 at 642.591 kb on + strand, within Pf1N1B4_544 at 642.592 kb on - strand, within Pf1N1B4_544 at 642.786 kb on + strand at 642.786 kb on + strand at 642.786 kb on + strand at 642.786 kb on + strand at 642.786 kb on + strand at 642.787 kb on - strand at 642.787 kb on - strand at 642.787 kb on - strand at 642.787 kb on - strand at 642.787 kb on - strand at 642.787 kb on - strand at 642.787 kb on - strand at 642.787 kb on - strand at 642.907 kb on - strand at 642.907 kb on - strand at 642.932 kb on + strand at 643.099 kb on + strand, within Pf1N1B4_545 at 643.228 kb on + strand, within Pf1N1B4_545 at 643.228 kb on + strand, within Pf1N1B4_545 at 643.228 kb on + strand, within Pf1N1B4_545 at 643.229 kb on - strand, within Pf1N1B4_545 at 643.229 kb on - strand, within Pf1N1B4_545 at 643.229 kb on - strand, within Pf1N1B4_545 at 643.229 kb on - strand, within Pf1N1B4_545 at 643.229 kb on - strand, within Pf1N1B4_545 at 643.235 kb on - strand, within Pf1N1B4_545 at 643.291 kb on + strand, within Pf1N1B4_545 at 643.291 kb on + strand, within Pf1N1B4_545 at 643.292 kb on - strand, within Pf1N1B4_545 at 643.534 kb on + strand, within Pf1N1B4_545 at 643.547 kb on - strand, within Pf1N1B4_545 at 643.547 kb on - strand, within Pf1N1B4_545 at 643.547 kb on - strand, within Pf1N1B4_545 at 643.547 kb on - strand at 643.675 kb on + strand at 643.676 kb on - strand at 643.676 kb on - strand at 643.700 kb on - strand at 643.700 kb on - strand at 643.749 kb on + strand at 643.750 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 641,317 - +1.1 641,335 - +0.2 641,372 - +0.9 641,373 + +0.9 641,373 + -0.1 641,440 + -1.4 641,440 + -0.1 641,440 + -1.4 641,441 - +0.2 641,441 - +0.7 641,441 - -0.7 641,602 + -1.1 641,603 - +0.4 641,786 + Pf1N1B4_544 0.23 -0.1 641,874 + Pf1N1B4_544 0.30 -0.1 642,378 + Pf1N1B4_544 0.68 -2.7 642,501 + +0.9 642,502 - Pf1N1B4_544 0.78 -1.2 642,591 + Pf1N1B4_544 0.85 -0.3 642,591 + Pf1N1B4_544 0.85 -0.4 642,592 - Pf1N1B4_544 0.85 -0.8 642,786 + -0.5 642,786 + -0.1 642,786 + +0.1 642,786 + +0.7 642,786 + +0.8 642,787 - -0.2 642,787 - +1.6 642,787 - -1.4 642,787 - +1.3 642,787 - -0.7 642,787 - -1.4 642,787 - -0.1 642,787 - +0.2 642,907 - +0.6 642,907 - +0.8 642,932 + -2.3 643,099 + Pf1N1B4_545 0.24 -0.1 643,228 + Pf1N1B4_545 0.39 -0.5 643,228 + Pf1N1B4_545 0.39 -0.9 643,228 + Pf1N1B4_545 0.39 +0.1 643,229 - Pf1N1B4_545 0.39 +0.6 643,229 - Pf1N1B4_545 0.39 -0.2 643,229 - Pf1N1B4_545 0.39 +1.0 643,229 - Pf1N1B4_545 0.39 -0.7 643,229 - Pf1N1B4_545 0.39 -1.7 643,235 - Pf1N1B4_545 0.39 -0.5 643,291 + Pf1N1B4_545 0.46 -0.8 643,291 + Pf1N1B4_545 0.46 -0.4 643,292 - Pf1N1B4_545 0.46 -1.3 643,534 + Pf1N1B4_545 0.74 -0.4 643,547 - Pf1N1B4_545 0.75 +0.0 643,547 - Pf1N1B4_545 0.75 -0.1 643,547 - Pf1N1B4_545 0.75 -0.1 643,547 - +0.9 643,675 + -0.1 643,676 - -0.5 643,676 - +0.9 643,700 - -1.2 643,700 - +0.4 643,749 + -1.1 643,750 - -0.2
Or see this region's nucleotide sequence