Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5427 and Pf1N1B4_5428 are separated by 474 nucleotides Pf1N1B4_5428 and Pf1N1B4_5429 are separated by 253 nucleotides Pf1N1B4_5429 and Pf1N1B4_5430 are separated by 85 nucleotides
Pf1N1B4_5427 - C4-type zinc finger protein, DksA/TraR family, at 166,711 to 167,184
_5427
Pf1N1B4_5428 - hypothetical protein, at 167,659 to 168,645
_5428
Pf1N1B4_5429 - hypothetical protein, at 168,899 to 169,543
_5429
Pf1N1B4_5430 - hypothetical protein, at 169,629 to 169,796
_5430
Position (kb) 167
168
169 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 166.819 kb on + strand, within Pf1N1B4_5427 at 166.819 kb on + strand, within Pf1N1B4_5427 at 166.820 kb on - strand, within Pf1N1B4_5427 at 166.820 kb on - strand, within Pf1N1B4_5427 at 166.893 kb on - strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.977 kb on - strand at 166.986 kb on + strand, within Pf1N1B4_5427 at 166.986 kb on + strand, within Pf1N1B4_5427 at 166.987 kb on - strand, within Pf1N1B4_5427 at 166.987 kb on - strand, within Pf1N1B4_5427 at 167.056 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.126 kb on - strand, within Pf1N1B4_5427 at 167.128 kb on - strand, within Pf1N1B4_5427 at 167.182 kb on + strand at 167.182 kb on + strand at 167.183 kb on - strand at 167.239 kb on - strand at 167.275 kb on - strand at 167.355 kb on - strand at 167.381 kb on - strand at 167.381 kb on - strand at 167.384 kb on - strand at 167.384 kb on - strand at 167.384 kb on - strand at 167.384 kb on - strand at 167.384 kb on - strand at 167.384 kb on - strand at 167.438 kb on - strand at 167.476 kb on - strand at 167.566 kb on - strand at 167.566 kb on - strand at 167.594 kb on + strand at 167.594 kb on + strand at 167.595 kb on - strand at 167.595 kb on - strand at 167.629 kb on + strand at 167.629 kb on + strand at 167.640 kb on - strand at 167.640 kb on - strand at 167.672 kb on - strand at 167.745 kb on + strand at 167.766 kb on + strand, within Pf1N1B4_5428 at 167.788 kb on + strand, within Pf1N1B4_5428 at 167.788 kb on + strand, within Pf1N1B4_5428 at 167.843 kb on - strand, within Pf1N1B4_5428 at 168.034 kb on + strand, within Pf1N1B4_5428 at 168.035 kb on - strand, within Pf1N1B4_5428 at 168.052 kb on + strand, within Pf1N1B4_5428 at 168.053 kb on - strand, within Pf1N1B4_5428 at 168.053 kb on - strand at 168.069 kb on + strand, within Pf1N1B4_5428 at 168.070 kb on - strand, within Pf1N1B4_5428 at 168.130 kb on + strand, within Pf1N1B4_5428 at 168.131 kb on - strand, within Pf1N1B4_5428 at 168.131 kb on - strand, within Pf1N1B4_5428 at 168.131 kb on - strand, within Pf1N1B4_5428 at 168.131 kb on - strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.134 kb on - strand, within Pf1N1B4_5428 at 168.134 kb on - strand, within Pf1N1B4_5428 at 168.145 kb on + strand, within Pf1N1B4_5428 at 168.196 kb on + strand, within Pf1N1B4_5428 at 168.197 kb on - strand, within Pf1N1B4_5428 at 168.197 kb on - strand, within Pf1N1B4_5428 at 168.197 kb on - strand, within Pf1N1B4_5428 at 168.197 kb on - strand, within Pf1N1B4_5428 at 168.201 kb on - strand, within Pf1N1B4_5428 at 168.381 kb on + strand, within Pf1N1B4_5428 at 168.391 kb on + strand, within Pf1N1B4_5428 at 168.415 kb on + strand, within Pf1N1B4_5428 at 168.415 kb on + strand, within Pf1N1B4_5428 at 168.416 kb on - strand, within Pf1N1B4_5428 at 168.416 kb on - strand, within Pf1N1B4_5428 at 168.419 kb on + strand, within Pf1N1B4_5428 at 168.419 kb on + strand, within Pf1N1B4_5428 at 168.419 kb on + strand, within Pf1N1B4_5428 at 168.419 kb on + strand, within Pf1N1B4_5428 at 168.419 kb on + strand, within Pf1N1B4_5428 at 168.420 kb on - strand, within Pf1N1B4_5428 at 168.524 kb on - strand, within Pf1N1B4_5428 at 168.524 kb on - strand, within Pf1N1B4_5428 at 168.580 kb on + strand at 168.581 kb on - strand at 168.660 kb on - strand at 168.728 kb on + strand at 168.729 kb on - strand at 168.731 kb on + strand at 168.731 kb on + strand at 168.731 kb on + strand at 168.732 kb on - strand at 168.747 kb on + strand at 168.747 kb on + strand at 168.906 kb on - strand at 168.977 kb on + strand, within Pf1N1B4_5429 at 168.978 kb on - strand, within Pf1N1B4_5429 at 169.103 kb on + strand, within Pf1N1B4_5429 at 169.103 kb on + strand, within Pf1N1B4_5429 at 169.175 kb on + strand, within Pf1N1B4_5429 at 169.175 kb on + strand, within Pf1N1B4_5429 at 169.175 kb on + strand, within Pf1N1B4_5429 at 169.175 kb on + strand, within Pf1N1B4_5429 at 169.175 kb on + strand, within Pf1N1B4_5429 at 169.176 kb on - strand, within Pf1N1B4_5429 at 169.304 kb on - strand, within Pf1N1B4_5429 at 169.304 kb on - strand, within Pf1N1B4_5429 at 169.329 kb on + strand, within Pf1N1B4_5429 at 169.337 kb on + strand, within Pf1N1B4_5429 at 169.337 kb on + strand, within Pf1N1B4_5429 at 169.337 kb on + strand, within Pf1N1B4_5429 at 169.338 kb on - strand, within Pf1N1B4_5429 at 169.549 kb on + strand at 169.549 kb on + strand at 169.549 kb on + strand at 169.549 kb on + strand at 169.549 kb on + strand at 169.584 kb on + strand at 169.584 kb on + strand at 169.584 kb on + strand at 169.585 kb on - strand at 169.585 kb on - strand at 169.631 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 166,819 + Pf1N1B4_5427 0.23 +0.5 166,819 + Pf1N1B4_5427 0.23 -0.9 166,820 - Pf1N1B4_5427 0.23 -0.7 166,820 - Pf1N1B4_5427 0.23 -0.1 166,893 - Pf1N1B4_5427 0.38 -0.1 166,976 + Pf1N1B4_5427 0.56 +1.0 166,976 + Pf1N1B4_5427 0.56 -0.1 166,976 + Pf1N1B4_5427 0.56 +0.3 166,976 + Pf1N1B4_5427 0.56 +1.6 166,976 + Pf1N1B4_5427 0.56 +1.0 166,976 + Pf1N1B4_5427 0.56 +0.6 166,976 + Pf1N1B4_5427 0.56 +0.4 166,976 + Pf1N1B4_5427 0.56 +1.4 166,976 + Pf1N1B4_5427 0.56 +1.1 166,977 - +1.9 166,986 + Pf1N1B4_5427 0.58 -2.3 166,986 + Pf1N1B4_5427 0.58 -0.6 166,987 - Pf1N1B4_5427 0.58 -0.5 166,987 - Pf1N1B4_5427 0.58 +0.1 167,056 + Pf1N1B4_5427 0.73 -0.9 167,125 + Pf1N1B4_5427 0.87 -0.9 167,125 + Pf1N1B4_5427 0.87 -0.7 167,125 + Pf1N1B4_5427 0.87 -0.2 167,125 + Pf1N1B4_5427 0.87 +0.4 167,125 + Pf1N1B4_5427 0.87 +1.0 167,125 + Pf1N1B4_5427 0.87 -1.0 167,125 + Pf1N1B4_5427 0.87 +1.4 167,126 - Pf1N1B4_5427 0.88 +1.4 167,128 - Pf1N1B4_5427 0.88 +2.4 167,182 + +0.4 167,182 + -1.7 167,183 - -0.0 167,239 - -1.5 167,275 - -0.1 167,355 - -1.1 167,381 - +0.5 167,381 - -0.3 167,384 - +0.5 167,384 - -0.6 167,384 - -0.0 167,384 - +0.3 167,384 - -2.3 167,384 - -0.5 167,438 - +1.3 167,476 - +0.7 167,566 - +0.0 167,566 - +0.9 167,594 + -1.6 167,594 + +0.9 167,595 - +0.7 167,595 - -0.4 167,629 + -0.3 167,629 + +0.0 167,640 - -0.0 167,640 - +0.1 167,672 - -0.3 167,745 + +1.1 167,766 + Pf1N1B4_5428 0.11 -0.3 167,788 + Pf1N1B4_5428 0.13 +1.0 167,788 + Pf1N1B4_5428 0.13 +0.4 167,843 - Pf1N1B4_5428 0.19 -0.2 168,034 + Pf1N1B4_5428 0.38 -1.1 168,035 - Pf1N1B4_5428 0.38 +3.7 168,052 + Pf1N1B4_5428 0.40 +0.0 168,053 - Pf1N1B4_5428 0.40 -1.5 168,053 - +1.9 168,069 + Pf1N1B4_5428 0.42 -1.7 168,070 - Pf1N1B4_5428 0.42 -0.7 168,130 + Pf1N1B4_5428 0.48 +0.4 168,131 - Pf1N1B4_5428 0.48 +1.9 168,131 - Pf1N1B4_5428 0.48 +1.1 168,131 - Pf1N1B4_5428 0.48 +0.2 168,131 - Pf1N1B4_5428 0.48 -0.1 168,133 + Pf1N1B4_5428 0.48 -0.6 168,133 + Pf1N1B4_5428 0.48 -1.5 168,133 + Pf1N1B4_5428 0.48 -1.0 168,133 + Pf1N1B4_5428 0.48 +0.0 168,133 + Pf1N1B4_5428 0.48 +2.0 168,133 + Pf1N1B4_5428 0.48 +1.9 168,134 - Pf1N1B4_5428 0.48 -0.1 168,134 - Pf1N1B4_5428 0.48 +1.5 168,145 + Pf1N1B4_5428 0.49 +0.4 168,196 + Pf1N1B4_5428 0.54 -1.7 168,197 - Pf1N1B4_5428 0.55 -0.9 168,197 - Pf1N1B4_5428 0.55 -0.4 168,197 - Pf1N1B4_5428 0.55 -0.2 168,197 - Pf1N1B4_5428 0.55 -1.5 168,201 - Pf1N1B4_5428 0.55 -1.2 168,381 + Pf1N1B4_5428 0.73 +0.9 168,391 + Pf1N1B4_5428 0.74 -0.3 168,415 + Pf1N1B4_5428 0.77 +0.5 168,415 + Pf1N1B4_5428 0.77 -1.0 168,416 - Pf1N1B4_5428 0.77 +0.3 168,416 - Pf1N1B4_5428 0.77 -1.5 168,419 + Pf1N1B4_5428 0.77 +0.4 168,419 + Pf1N1B4_5428 0.77 -1.3 168,419 + Pf1N1B4_5428 0.77 +1.6 168,419 + Pf1N1B4_5428 0.77 -1.3 168,419 + Pf1N1B4_5428 0.77 -0.1 168,420 - Pf1N1B4_5428 0.77 +0.0 168,524 - Pf1N1B4_5428 0.88 -0.4 168,524 - Pf1N1B4_5428 0.88 +2.2 168,580 + +0.5 168,581 - -2.5 168,660 - +0.6 168,728 + +0.3 168,729 - +0.4 168,731 + -2.6 168,731 + +0.3 168,731 + -0.1 168,732 - -0.1 168,747 + -0.6 168,747 + +0.3 168,906 - +0.9 168,977 + Pf1N1B4_5429 0.12 -0.3 168,978 - Pf1N1B4_5429 0.12 +0.5 169,103 + Pf1N1B4_5429 0.32 -0.5 169,103 + Pf1N1B4_5429 0.32 +0.1 169,175 + Pf1N1B4_5429 0.43 +0.7 169,175 + Pf1N1B4_5429 0.43 -1.1 169,175 + Pf1N1B4_5429 0.43 -1.1 169,175 + Pf1N1B4_5429 0.43 -0.3 169,175 + Pf1N1B4_5429 0.43 +0.5 169,176 - Pf1N1B4_5429 0.43 -0.1 169,304 - Pf1N1B4_5429 0.63 +1.0 169,304 - Pf1N1B4_5429 0.63 -2.5 169,329 + Pf1N1B4_5429 0.67 +1.7 169,337 + Pf1N1B4_5429 0.68 -1.5 169,337 + Pf1N1B4_5429 0.68 -1.5 169,337 + Pf1N1B4_5429 0.68 -0.1 169,338 - Pf1N1B4_5429 0.68 +0.3 169,549 + +0.4 169,549 + +0.9 169,549 + -1.5 169,549 + -0.3 169,549 + -1.1 169,584 + +1.5 169,584 + -1.5 169,584 + +0.3 169,585 - -0.7 169,585 - +0.7 169,631 - -1.5
Or see this region's nucleotide sequence