Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5405 and Pf1N1B4_5406 are separated by 875 nucleotides Pf1N1B4_5406 and Pf1N1B4_5407 are separated by 379 nucleotides Pf1N1B4_5407 and Pf1N1B4_5408 are separated by 69 nucleotides Pf1N1B4_5408 and Pf1N1B4_5409 are separated by 38 nucleotides
Pf1N1B4_5405 - hypothetical protein, at 140,141 to 141,181
_5405
Pf1N1B4_5406 - hypothetical protein, at 142,057 to 142,173
_5406
Pf1N1B4_5407 - hypothetical protein, at 142,553 to 142,762
_5407
Pf1N1B4_5408 - hypothetical protein, at 142,832 to 143,065
_5408
Pf1N1B4_5409 - hypothetical protein, at 143,104 to 143,562
_5409
Position (kb) 142
143 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 141.074 kb on + strand, within Pf1N1B4_5405 at 141.074 kb on + strand, within Pf1N1B4_5405 at 141.074 kb on + strand, within Pf1N1B4_5405 at 141.074 kb on + strand, within Pf1N1B4_5405 at 141.074 kb on + strand, within Pf1N1B4_5405 at 141.075 kb on - strand, within Pf1N1B4_5405 at 141.088 kb on + strand at 141.088 kb on + strand at 141.089 kb on - strand at 141.089 kb on - strand at 141.092 kb on + strand at 141.092 kb on + strand at 141.092 kb on + strand at 141.092 kb on + strand at 141.092 kb on + strand at 141.092 kb on + strand at 141.093 kb on - strand at 141.093 kb on - strand at 141.093 kb on - strand at 141.093 kb on - strand at 141.093 kb on - strand at 141.093 kb on - strand at 141.093 kb on - strand at 141.131 kb on + strand at 141.134 kb on + strand at 141.156 kb on + strand at 141.156 kb on + strand at 141.156 kb on + strand at 141.157 kb on - strand at 141.157 kb on - strand at 141.160 kb on + strand at 141.160 kb on + strand at 141.160 kb on + strand at 141.160 kb on + strand at 141.160 kb on + strand at 141.160 kb on + strand at 141.160 kb on + strand at 141.161 kb on - strand at 141.296 kb on + strand at 141.297 kb on - strand at 141.297 kb on - strand at 141.350 kb on - strand at 141.350 kb on - strand at 141.350 kb on - strand at 141.363 kb on - strand at 141.415 kb on + strand at 141.415 kb on + strand at 141.415 kb on + strand at 141.415 kb on + strand at 141.415 kb on + strand at 141.416 kb on - strand at 141.416 kb on - strand at 141.416 kb on - strand at 141.416 kb on - strand at 141.427 kb on + strand at 141.428 kb on - strand at 141.436 kb on + strand at 141.437 kb on - strand at 141.438 kb on + strand at 141.438 kb on + strand at 141.438 kb on + strand at 141.438 kb on + strand at 141.438 kb on + strand at 141.438 kb on + strand at 141.438 kb on + strand at 141.438 kb on + strand at 141.438 kb on + strand at 141.438 kb on + strand at 141.438 kb on + strand at 141.439 kb on - strand at 141.439 kb on - strand at 141.439 kb on - strand at 141.439 kb on - strand at 141.439 kb on - strand at 141.439 kb on - strand at 141.487 kb on - strand at 141.621 kb on + strand at 141.621 kb on + strand at 141.621 kb on + strand at 141.621 kb on + strand at 141.621 kb on + strand at 141.622 kb on - strand at 141.624 kb on + strand at 141.625 kb on - strand at 141.625 kb on - strand at 141.653 kb on + strand at 141.654 kb on - strand at 141.654 kb on - strand at 141.654 kb on - strand at 141.654 kb on - strand at 141.654 kb on - strand at 141.668 kb on + strand at 141.668 kb on + strand at 141.668 kb on + strand at 141.668 kb on + strand at 141.669 kb on - strand at 141.669 kb on - strand at 141.669 kb on - strand at 141.725 kb on + strand at 141.725 kb on + strand at 141.725 kb on + strand at 141.725 kb on + strand at 141.725 kb on + strand at 141.725 kb on + strand at 141.725 kb on + strand at 141.725 kb on + strand at 141.725 kb on + strand at 141.726 kb on - strand at 141.726 kb on - strand at 141.726 kb on - strand at 141.726 kb on - strand at 141.726 kb on - strand at 141.726 kb on - strand at 141.774 kb on + strand at 141.791 kb on + strand at 141.792 kb on - strand at 141.813 kb on + strand at 141.816 kb on - strand at 141.816 kb on - strand at 141.899 kb on + strand at 141.899 kb on + strand at 141.900 kb on - strand at 141.900 kb on - strand at 141.920 kb on - strand at 141.970 kb on + strand at 141.971 kb on - strand at 141.971 kb on - strand at 141.995 kb on + strand at 141.996 kb on - strand at 141.996 kb on - strand at 142.098 kb on + strand, within Pf1N1B4_5406 at 142.098 kb on + strand, within Pf1N1B4_5406 at 142.098 kb on + strand, within Pf1N1B4_5406 at 142.098 kb on + strand, within Pf1N1B4_5406 at 142.098 kb on + strand, within Pf1N1B4_5406 at 142.098 kb on + strand, within Pf1N1B4_5406 at 142.098 kb on + strand, within Pf1N1B4_5406 at 142.099 kb on - strand, within Pf1N1B4_5406 at 142.099 kb on - strand, within Pf1N1B4_5406 at 142.099 kb on - strand, within Pf1N1B4_5406 at 142.099 kb on - strand, within Pf1N1B4_5406 at 142.099 kb on - strand, within Pf1N1B4_5406 at 142.099 kb on - strand, within Pf1N1B4_5406 at 142.099 kb on - strand, within Pf1N1B4_5406 at 142.268 kb on + strand at 142.269 kb on - strand at 142.269 kb on - strand at 142.357 kb on + strand at 142.357 kb on + strand at 142.358 kb on - strand at 142.452 kb on - strand at 142.523 kb on - strand at 142.593 kb on + strand, within Pf1N1B4_5407 at 142.665 kb on + strand, within Pf1N1B4_5407 at 142.665 kb on + strand, within Pf1N1B4_5407 at 142.666 kb on - strand, within Pf1N1B4_5407 at 142.666 kb on - strand, within Pf1N1B4_5407 at 142.666 kb on - strand at 142.766 kb on + strand at 142.766 kb on + strand at 142.766 kb on + strand at 142.766 kb on + strand at 142.766 kb on + strand at 142.766 kb on + strand at 142.767 kb on - strand at 142.767 kb on - strand at 142.819 kb on - strand at 142.877 kb on + strand, within Pf1N1B4_5408 at 142.877 kb on + strand, within Pf1N1B4_5408 at 142.877 kb on + strand, within Pf1N1B4_5408 at 142.878 kb on - strand, within Pf1N1B4_5408 at 142.878 kb on - strand, within Pf1N1B4_5408 at 142.878 kb on - strand at 142.879 kb on + strand, within Pf1N1B4_5408 at 142.879 kb on + strand at 142.880 kb on - strand, within Pf1N1B4_5408 at 142.982 kb on - strand, within Pf1N1B4_5408 at 142.989 kb on + strand, within Pf1N1B4_5408 at 142.989 kb on + strand, within Pf1N1B4_5408 at 142.990 kb on - strand, within Pf1N1B4_5408 at 143.064 kb on - strand at 143.081 kb on + strand at 143.134 kb on - strand at 143.134 kb on - strand at 143.134 kb on - strand at 143.169 kb on - strand, within Pf1N1B4_5409
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 141,074 + Pf1N1B4_5405 0.90 +0.3 141,074 + Pf1N1B4_5405 0.90 +1.7 141,074 + Pf1N1B4_5405 0.90 +0.1 141,074 + Pf1N1B4_5405 0.90 -0.7 141,074 + Pf1N1B4_5405 0.90 +0.9 141,075 - Pf1N1B4_5405 0.90 -0.4 141,088 + -0.5 141,088 + -1.1 141,089 - +1.5 141,089 - +0.7 141,092 + -1.0 141,092 + +0.6 141,092 + +0.9 141,092 + -0.1 141,092 + +0.9 141,092 + -0.5 141,093 - +0.6 141,093 - -2.0 141,093 - -0.7 141,093 - -1.6 141,093 - +2.0 141,093 - +0.1 141,093 - +0.9 141,131 + +0.7 141,134 + +0.3 141,156 + -1.3 141,156 + -0.5 141,156 + -1.7 141,157 - -0.1 141,157 - +0.3 141,160 + +0.3 141,160 + +0.0 141,160 + -0.9 141,160 + +0.3 141,160 + +1.0 141,160 + -0.1 141,160 + +1.5 141,161 - +1.2 141,296 + +0.3 141,297 - -1.1 141,297 - +0.6 141,350 - -0.7 141,350 - +0.9 141,350 - +0.2 141,363 - -0.4 141,415 + -0.1 141,415 + +0.1 141,415 + +2.2 141,415 + +1.6 141,415 + +0.5 141,416 - -0.3 141,416 - +1.6 141,416 - +0.4 141,416 - +0.3 141,427 + +0.9 141,428 - +0.9 141,436 + -0.4 141,437 - -0.1 141,438 + +0.9 141,438 + +0.0 141,438 + +2.3 141,438 + -0.6 141,438 + -0.6 141,438 + +1.2 141,438 + +1.4 141,438 + -0.4 141,438 + -0.7 141,438 + -0.7 141,438 + +0.3 141,439 - +1.3 141,439 - -0.1 141,439 - +3.9 141,439 - -0.4 141,439 - -0.6 141,439 - +1.3 141,487 - -0.7 141,621 + +1.1 141,621 + -0.1 141,621 + -0.7 141,621 + +0.7 141,621 + +2.2 141,622 - -0.3 141,624 + +1.7 141,625 - +0.3 141,625 - -1.5 141,653 + -2.0 141,654 - +0.9 141,654 - +0.9 141,654 - +0.5 141,654 - +0.9 141,654 - -2.0 141,668 + -0.4 141,668 + +1.1 141,668 + +0.6 141,668 + -0.5 141,669 - -1.1 141,669 - +0.0 141,669 - +1.7 141,725 + -1.9 141,725 + -0.5 141,725 + +0.6 141,725 + +0.2 141,725 + +0.0 141,725 + -0.6 141,725 + -1.5 141,725 + +1.1 141,725 + -0.2 141,726 - -0.7 141,726 - +0.9 141,726 - -0.1 141,726 - +0.5 141,726 - -1.5 141,726 - -0.7 141,774 + +0.4 141,791 + -2.1 141,792 - -0.7 141,813 + +0.7 141,816 - +0.9 141,816 - -0.1 141,899 + -0.4 141,899 + -0.1 141,900 - +1.1 141,900 - +1.1 141,920 - -2.1 141,970 + -0.7 141,971 - -1.5 141,971 - -0.9 141,995 + -0.0 141,996 - -1.0 141,996 - -0.6 142,098 + Pf1N1B4_5406 0.35 +1.4 142,098 + Pf1N1B4_5406 0.35 +0.6 142,098 + Pf1N1B4_5406 0.35 +1.7 142,098 + Pf1N1B4_5406 0.35 -0.2 142,098 + Pf1N1B4_5406 0.35 +1.0 142,098 + Pf1N1B4_5406 0.35 +0.4 142,098 + Pf1N1B4_5406 0.35 -0.0 142,099 - Pf1N1B4_5406 0.36 -0.3 142,099 - Pf1N1B4_5406 0.36 +0.4 142,099 - Pf1N1B4_5406 0.36 -0.7 142,099 - Pf1N1B4_5406 0.36 -0.7 142,099 - Pf1N1B4_5406 0.36 -0.5 142,099 - Pf1N1B4_5406 0.36 +1.2 142,099 - Pf1N1B4_5406 0.36 +1.7 142,268 + -0.5 142,269 - +0.5 142,269 - +0.9 142,357 + +0.6 142,357 + +0.2 142,358 - -0.7 142,452 - -2.3 142,523 - +0.0 142,593 + Pf1N1B4_5407 0.19 -0.8 142,665 + Pf1N1B4_5407 0.53 +0.1 142,665 + Pf1N1B4_5407 0.53 +0.2 142,666 - Pf1N1B4_5407 0.54 -2.7 142,666 - Pf1N1B4_5407 0.54 -2.1 142,666 - +1.3 142,766 + -0.1 142,766 + +1.4 142,766 + +1.0 142,766 + -0.1 142,766 + +0.7 142,766 + +0.2 142,767 - +2.1 142,767 - +0.9 142,819 - -0.7 142,877 + Pf1N1B4_5408 0.19 -1.5 142,877 + Pf1N1B4_5408 0.19 -2.0 142,877 + Pf1N1B4_5408 0.19 -0.3 142,878 - Pf1N1B4_5408 0.20 -1.0 142,878 - Pf1N1B4_5408 0.20 -2.1 142,878 - +1.1 142,879 + Pf1N1B4_5408 0.20 +1.2 142,879 + +0.3 142,880 - Pf1N1B4_5408 0.21 -1.6 142,982 - Pf1N1B4_5408 0.64 +1.3 142,989 + Pf1N1B4_5408 0.67 -2.1 142,989 + Pf1N1B4_5408 0.67 +0.5 142,990 - Pf1N1B4_5408 0.68 -0.1 143,064 - -1.1 143,081 + -0.2 143,134 - +0.7 143,134 - -0.1 143,134 - +0.3 143,169 - Pf1N1B4_5409 0.14 -2.1
Or see this region's nucleotide sequence