Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_539

Experiment: Spectinomycin 0.05 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_538 and Pf1N1B4_539 are separated by 120 nucleotidesPf1N1B4_539 and Pf1N1B4_540 are separated by 201 nucleotides Pf1N1B4_538 - hypothetical protein, at 636,352 to 637,221 _538 Pf1N1B4_539 - Transcriptional regulators, at 637,342 to 637,734 _539 Pf1N1B4_540 - FIG00960825: hypothetical protein, at 637,936 to 638,670 _540 Position (kb) 637 638Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 636.475 kb on + strand, within Pf1N1B4_538at 636.476 kb on - strand, within Pf1N1B4_538at 636.699 kb on - strandat 636.784 kb on + strand, within Pf1N1B4_538at 637.219 kb on + strandat 637.219 kb on + strandat 637.219 kb on + strandat 637.219 kb on + strandat 637.219 kb on + strandat 637.220 kb on - strandat 637.226 kb on + strandat 637.232 kb on + strandat 637.233 kb on - strandat 637.233 kb on - strandat 637.494 kb on - strand, within Pf1N1B4_539at 637.539 kb on - strand, within Pf1N1B4_539at 637.638 kb on - strandat 637.649 kb on + strand, within Pf1N1B4_539at 637.649 kb on + strand, within Pf1N1B4_539at 637.650 kb on - strand, within Pf1N1B4_539at 637.723 kb on - strandat 637.723 kb on - strandat 637.723 kb on - strandat 637.724 kb on + strandat 637.724 kb on + strandat 637.724 kb on + strandat 637.724 kb on + strandat 637.724 kb on + strandat 637.725 kb on - strandat 637.725 kb on - strandat 637.725 kb on - strandat 637.725 kb on - strandat 637.725 kb on - strandat 637.725 kb on - strandat 637.725 kb on - strandat 637.725 kb on - strandat 637.725 kb on - strandat 637.775 kb on + strandat 637.775 kb on + strandat 637.775 kb on + strandat 637.775 kb on + strandat 637.776 kb on - strandat 637.776 kb on - strandat 637.776 kb on - strandat 637.776 kb on - strandat 637.776 kb on - strandat 637.776 kb on - strandat 637.776 kb on - strandat 637.776 kb on - strandat 637.776 kb on - strandat 637.776 kb on - strandat 637.776 kb on - strandat 637.815 kb on + strandat 637.868 kb on - strandat 637.876 kb on + strandat 637.876 kb on + strandat 637.876 kb on + strandat 637.877 kb on - strandat 637.877 kb on - strandat 637.877 kb on - strandat 637.877 kb on - strandat 637.886 kb on + strandat 638.158 kb on + strand, within Pf1N1B4_540at 638.158 kb on + strand, within Pf1N1B4_540at 638.158 kb on + strand, within Pf1N1B4_540at 638.159 kb on - strand, within Pf1N1B4_540at 638.159 kb on - strandat 638.159 kb on - strand, within Pf1N1B4_540at 638.159 kb on - strandat 638.159 kb on - strand, within Pf1N1B4_540at 638.159 kb on - strand, within Pf1N1B4_540at 638.159 kb on - strand, within Pf1N1B4_540at 638.368 kb on + strand, within Pf1N1B4_540at 638.369 kb on - strand, within Pf1N1B4_540at 638.369 kb on - strandat 638.369 kb on - strand, within Pf1N1B4_540at 638.369 kb on - strand, within Pf1N1B4_540at 638.369 kb on - strand, within Pf1N1B4_540at 638.369 kb on - strand, within Pf1N1B4_540at 638.418 kb on + strand, within Pf1N1B4_540at 638.418 kb on + strand, within Pf1N1B4_540at 638.419 kb on - strand, within Pf1N1B4_540at 638.419 kb on - strand, within Pf1N1B4_540at 638.419 kb on - strand, within Pf1N1B4_540at 638.419 kb on - strand, within Pf1N1B4_540at 638.422 kb on + strand, within Pf1N1B4_540at 638.422 kb on + strand, within Pf1N1B4_540at 638.422 kb on + strand, within Pf1N1B4_540at 638.422 kb on + strand, within Pf1N1B4_540at 638.422 kb on + strand, within Pf1N1B4_540at 638.422 kb on + strand, within Pf1N1B4_540at 638.422 kb on + strand, within Pf1N1B4_540at 638.422 kb on + strand, within Pf1N1B4_540at 638.422 kb on + strand, within Pf1N1B4_540at 638.422 kb on + strand, within Pf1N1B4_540at 638.423 kb on - strand, within Pf1N1B4_540at 638.423 kb on - strandat 638.423 kb on - strand, within Pf1N1B4_540at 638.423 kb on - strand, within Pf1N1B4_540at 638.423 kb on - strand, within Pf1N1B4_540at 638.423 kb on - strand, within Pf1N1B4_540at 638.423 kb on - strand, within Pf1N1B4_540at 638.423 kb on - strand, within Pf1N1B4_540at 638.423 kb on - strand, within Pf1N1B4_540at 638.423 kb on - strand, within Pf1N1B4_540at 638.423 kb on - strand, within Pf1N1B4_540at 638.423 kb on - strand, within Pf1N1B4_540at 638.429 kb on - strand, within Pf1N1B4_540at 638.429 kb on - strand, within Pf1N1B4_540at 638.512 kb on + strand, within Pf1N1B4_540at 638.512 kb on + strand, within Pf1N1B4_540at 638.512 kb on + strand, within Pf1N1B4_540at 638.512 kb on + strand, within Pf1N1B4_540at 638.512 kb on + strand, within Pf1N1B4_540at 638.512 kb on + strand, within Pf1N1B4_540at 638.512 kb on + strand, within Pf1N1B4_540at 638.512 kb on + strand, within Pf1N1B4_540at 638.513 kb on - strandat 638.513 kb on - strand, within Pf1N1B4_540at 638.521 kb on + strand, within Pf1N1B4_540at 638.521 kb on + strand, within Pf1N1B4_540at 638.521 kb on + strand, within Pf1N1B4_540at 638.521 kb on + strand, within Pf1N1B4_540at 638.521 kb on + strand, within Pf1N1B4_540at 638.521 kb on + strand, within Pf1N1B4_540at 638.521 kb on + strand, within Pf1N1B4_540at 638.521 kb on + strand, within Pf1N1B4_540at 638.521 kb on + strand, within Pf1N1B4_540at 638.521 kb on + strand, within Pf1N1B4_540at 638.521 kb on + strand, within Pf1N1B4_540at 638.521 kb on + strand, within Pf1N1B4_540at 638.522 kb on - strand, within Pf1N1B4_540at 638.522 kb on - strand, within Pf1N1B4_540at 638.522 kb on - strand, within Pf1N1B4_540at 638.522 kb on - strand, within Pf1N1B4_540at 638.522 kb on - strand, within Pf1N1B4_540at 638.522 kb on - strand, within Pf1N1B4_540at 638.522 kb on - strand, within Pf1N1B4_540at 638.522 kb on - strand, within Pf1N1B4_540at 638.522 kb on - strand, within Pf1N1B4_540at 638.522 kb on - strand, within Pf1N1B4_540at 638.522 kb on - strand, within Pf1N1B4_540at 638.554 kb on + strand, within Pf1N1B4_540at 638.554 kb on + strand, within Pf1N1B4_540at 638.554 kb on + strand, within Pf1N1B4_540at 638.554 kb on + strand, within Pf1N1B4_540at 638.554 kb on + strand, within Pf1N1B4_540at 638.555 kb on - strand, within Pf1N1B4_540at 638.555 kb on - strand, within Pf1N1B4_540at 638.555 kb on - strand, within Pf1N1B4_540at 638.555 kb on - strand, within Pf1N1B4_540at 638.555 kb on - strand, within Pf1N1B4_540at 638.560 kb on + strand, within Pf1N1B4_540at 638.560 kb on + strand, within Pf1N1B4_540at 638.560 kb on + strand, within Pf1N1B4_540at 638.560 kb on + strandat 638.560 kb on + strand, within Pf1N1B4_540at 638.561 kb on - strand, within Pf1N1B4_540at 638.561 kb on - strand, within Pf1N1B4_540at 638.561 kb on - strand, within Pf1N1B4_540at 638.587 kb on - strandat 638.669 kb on - strandat 638.669 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml
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636,475 + Pf1N1B4_538 0.14 -0.3
636,476 - Pf1N1B4_538 0.14 +0.9
636,699 - -0.1
636,784 + Pf1N1B4_538 0.50 +0.1
637,219 + +0.6
637,219 + +0.2
637,219 + -0.9
637,219 + +1.7
637,219 + +0.8
637,220 - +0.1
637,226 + -0.7
637,232 + +0.2
637,233 - +1.9
637,233 - +0.3
637,494 - Pf1N1B4_539 0.39 +0.2
637,539 - Pf1N1B4_539 0.50 +0.2
637,638 - -0.1
637,649 + Pf1N1B4_539 0.78 -1.4
637,649 + Pf1N1B4_539 0.78 -0.9
637,650 - Pf1N1B4_539 0.78 -2.3
637,723 - -0.5
637,723 - -1.1
637,723 - +0.0
637,724 + +0.9
637,724 + -0.7
637,724 + -1.1
637,724 + -1.1
637,724 + +1.5
637,725 - -0.4
637,725 - -0.5
637,725 - -2.6
637,725 - +1.6
637,725 - -0.5
637,725 - +0.2
637,725 - -0.4
637,725 - +1.2
637,725 - +0.3
637,775 + +1.1
637,775 + +1.0
637,775 + -0.6
637,775 + -1.4
637,776 - -1.2
637,776 - -0.8
637,776 - -0.4
637,776 - -1.3
637,776 - -0.7
637,776 - -0.4
637,776 - +1.3
637,776 - +0.9
637,776 - -1.9
637,776 - -1.7
637,776 - -0.8
637,815 + +1.2
637,868 - +0.8
637,876 + +1.9
637,876 + +0.5
637,876 + +0.9
637,877 - -0.1
637,877 - +0.9
637,877 - -0.4
637,877 - +0.0
637,886 + -0.7
638,158 + Pf1N1B4_540 0.30 -0.7
638,158 + Pf1N1B4_540 0.30 -0.6
638,158 + Pf1N1B4_540 0.30 +2.6
638,159 - Pf1N1B4_540 0.30 +0.6
638,159 - -1.9
638,159 - Pf1N1B4_540 0.30 -0.2
638,159 - +0.9
638,159 - Pf1N1B4_540 0.30 +1.3
638,159 - Pf1N1B4_540 0.30 -0.3
638,159 - Pf1N1B4_540 0.30 -0.1
638,368 + Pf1N1B4_540 0.59 -0.2
638,369 - Pf1N1B4_540 0.59 -0.1
638,369 - +0.6
638,369 - Pf1N1B4_540 0.59 -2.3
638,369 - Pf1N1B4_540 0.59 +0.7
638,369 - Pf1N1B4_540 0.59 +0.2
638,369 - Pf1N1B4_540 0.59 -0.2
638,418 + Pf1N1B4_540 0.66 +3.1
638,418 + Pf1N1B4_540 0.66 +0.9
638,419 - Pf1N1B4_540 0.66 +0.7
638,419 - Pf1N1B4_540 0.66 -0.3
638,419 - Pf1N1B4_540 0.66 +0.1
638,419 - Pf1N1B4_540 0.66 -1.6
638,422 + Pf1N1B4_540 0.66 -0.7
638,422 + Pf1N1B4_540 0.66 -0.9
638,422 + Pf1N1B4_540 0.66 -1.9
638,422 + Pf1N1B4_540 0.66 -1.4
638,422 + Pf1N1B4_540 0.66 -0.8
638,422 + Pf1N1B4_540 0.66 -0.8
638,422 + Pf1N1B4_540 0.66 -0.4
638,422 + Pf1N1B4_540 0.66 -0.4
638,422 + Pf1N1B4_540 0.66 -0.6
638,422 + Pf1N1B4_540 0.66 +0.7
638,423 - Pf1N1B4_540 0.66 -0.7
638,423 - +0.9
638,423 - Pf1N1B4_540 0.66 +1.4
638,423 - Pf1N1B4_540 0.66 +0.1
638,423 - Pf1N1B4_540 0.66 +2.3
638,423 - Pf1N1B4_540 0.66 -0.9
638,423 - Pf1N1B4_540 0.66 -0.5
638,423 - Pf1N1B4_540 0.66 +1.2
638,423 - Pf1N1B4_540 0.66 +0.3
638,423 - Pf1N1B4_540 0.66 -0.1
638,423 - Pf1N1B4_540 0.66 -0.1
638,423 - Pf1N1B4_540 0.66 -0.7
638,429 - Pf1N1B4_540 0.67 +0.5
638,429 - Pf1N1B4_540 0.67 +1.3
638,512 + Pf1N1B4_540 0.78 +0.1
638,512 + Pf1N1B4_540 0.78 -0.1
638,512 + Pf1N1B4_540 0.78 -0.7
638,512 + Pf1N1B4_540 0.78 -0.7
638,512 + Pf1N1B4_540 0.78 -1.1
638,512 + Pf1N1B4_540 0.78 -0.1
638,512 + Pf1N1B4_540 0.78 -0.3
638,512 + Pf1N1B4_540 0.78 +0.9
638,513 - -0.7
638,513 - Pf1N1B4_540 0.79 -0.4
638,521 + Pf1N1B4_540 0.80 +1.8
638,521 + Pf1N1B4_540 0.80 +0.2
638,521 + Pf1N1B4_540 0.80 -1.6
638,521 + Pf1N1B4_540 0.80 +1.2
638,521 + Pf1N1B4_540 0.80 +0.9
638,521 + Pf1N1B4_540 0.80 +0.2
638,521 + Pf1N1B4_540 0.80 +0.6
638,521 + Pf1N1B4_540 0.80 -0.2
638,521 + Pf1N1B4_540 0.80 -0.7
638,521 + Pf1N1B4_540 0.80 +0.3
638,521 + Pf1N1B4_540 0.80 -0.5
638,521 + Pf1N1B4_540 0.80 +1.6
638,522 - Pf1N1B4_540 0.80 -1.1
638,522 - Pf1N1B4_540 0.80 -0.5
638,522 - Pf1N1B4_540 0.80 +0.3
638,522 - Pf1N1B4_540 0.80 -0.7
638,522 - Pf1N1B4_540 0.80 +0.1
638,522 - Pf1N1B4_540 0.80 -1.1
638,522 - Pf1N1B4_540 0.80 -0.2
638,522 - Pf1N1B4_540 0.80 +0.9
638,522 - Pf1N1B4_540 0.80 -0.7
638,522 - Pf1N1B4_540 0.80 +0.2
638,522 - Pf1N1B4_540 0.80 -0.7
638,554 + Pf1N1B4_540 0.84 -1.9
638,554 + Pf1N1B4_540 0.84 +0.0
638,554 + Pf1N1B4_540 0.84 +0.3
638,554 + Pf1N1B4_540 0.84 +1.2
638,554 + Pf1N1B4_540 0.84 +0.4
638,555 - Pf1N1B4_540 0.84 +0.9
638,555 - Pf1N1B4_540 0.84 -0.4
638,555 - Pf1N1B4_540 0.84 -1.8
638,555 - Pf1N1B4_540 0.84 -0.6
638,555 - Pf1N1B4_540 0.84 -0.1
638,560 + Pf1N1B4_540 0.85 -1.7
638,560 + Pf1N1B4_540 0.85 +0.2
638,560 + Pf1N1B4_540 0.85 -1.2
638,560 + -0.1
638,560 + Pf1N1B4_540 0.85 +0.1
638,561 - Pf1N1B4_540 0.85 +2.2
638,561 - Pf1N1B4_540 0.85 +0.6
638,561 - Pf1N1B4_540 0.85 -0.2
638,587 - -0.1
638,669 - -2.9
638,669 - +0.2

Or see this region's nucleotide sequence