Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5268 and Pf1N1B4_5269 are separated by 330 nucleotides Pf1N1B4_5269 and Pf1N1B4_5270 are separated by 604 nucleotides
Pf1N1B4_5268 - hypothetical protein, at 25,619 to 26,140
_5268
Pf1N1B4_5269 - hypothetical protein, at 26,471 to 26,764
_5269
Pf1N1B4_5270 - hypothetical protein, at 27,369 to 28,193
_5270
Position (kb)
26
27 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 25.476 kb on + strand at 25.575 kb on + strand at 25.841 kb on + strand, within Pf1N1B4_5268 at 25.850 kb on + strand, within Pf1N1B4_5268 at 25.850 kb on + strand, within Pf1N1B4_5268 at 25.850 kb on + strand, within Pf1N1B4_5268 at 25.850 kb on + strand, within Pf1N1B4_5268 at 25.979 kb on + strand, within Pf1N1B4_5268 at 25.979 kb on + strand, within Pf1N1B4_5268 at 25.979 kb on + strand, within Pf1N1B4_5268 at 25.989 kb on + strand, within Pf1N1B4_5268 at 25.990 kb on - strand, within Pf1N1B4_5268 at 25.990 kb on - strand, within Pf1N1B4_5268 at 26.054 kb on + strand, within Pf1N1B4_5268 at 26.054 kb on + strand, within Pf1N1B4_5268 at 26.054 kb on + strand, within Pf1N1B4_5268 at 26.055 kb on - strand, within Pf1N1B4_5268 at 26.058 kb on + strand at 26.058 kb on + strand, within Pf1N1B4_5268 at 26.058 kb on + strand, within Pf1N1B4_5268 at 26.058 kb on + strand, within Pf1N1B4_5268 at 26.058 kb on + strand, within Pf1N1B4_5268 at 26.058 kb on + strand, within Pf1N1B4_5268 at 26.059 kb on - strand, within Pf1N1B4_5268 at 26.059 kb on - strand, within Pf1N1B4_5268 at 26.128 kb on - strand at 26.128 kb on - strand at 26.166 kb on + strand at 26.166 kb on + strand at 26.167 kb on - strand at 26.189 kb on + strand at 26.189 kb on + strand at 26.190 kb on - strand at 26.190 kb on - strand at 26.227 kb on - strand at 26.348 kb on + strand at 26.348 kb on + strand at 26.382 kb on - strand at 26.385 kb on + strand at 26.386 kb on - strand at 26.521 kb on + strand, within Pf1N1B4_5269 at 26.521 kb on + strand, within Pf1N1B4_5269 at 26.521 kb on + strand, within Pf1N1B4_5269 at 26.522 kb on - strand, within Pf1N1B4_5269 at 26.660 kb on + strand, within Pf1N1B4_5269 at 26.660 kb on + strand, within Pf1N1B4_5269 at 26.660 kb on + strand, within Pf1N1B4_5269 at 26.661 kb on - strand, within Pf1N1B4_5269 at 26.837 kb on - strand at 26.980 kb on - strand at 26.980 kb on - strand at 26.980 kb on - strand at 27.045 kb on + strand at 27.046 kb on - strand at 27.046 kb on - strand at 27.046 kb on - strand at 27.046 kb on - strand at 27.046 kb on - strand at 27.145 kb on + strand at 27.146 kb on - strand at 27.181 kb on + strand at 27.182 kb on - strand at 27.190 kb on - strand at 27.190 kb on - strand at 27.190 kb on - strand at 27.238 kb on - strand at 27.269 kb on + strand at 27.294 kb on - strand at 27.332 kb on - strand at 27.365 kb on + strand at 27.366 kb on - strand at 27.433 kb on - strand at 27.493 kb on + strand, within Pf1N1B4_5270 at 27.500 kb on + strand at 27.594 kb on + strand, within Pf1N1B4_5270 at 27.595 kb on - strand at 27.613 kb on - strand, within Pf1N1B4_5270 at 27.637 kb on - strand, within Pf1N1B4_5270 at 27.672 kb on + strand, within Pf1N1B4_5270 at 27.693 kb on + strand at 27.694 kb on - strand, within Pf1N1B4_5270 at 27.694 kb on - strand, within Pf1N1B4_5270 at 27.746 kb on + strand, within Pf1N1B4_5270 at 27.746 kb on + strand, within Pf1N1B4_5270 at 27.747 kb on - strand, within Pf1N1B4_5270 at 27.759 kb on - strand, within Pf1N1B4_5270 at 27.759 kb on - strand, within Pf1N1B4_5270
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 25,476 + -2.6 25,575 + -0.0 25,841 + Pf1N1B4_5268 0.43 -0.4 25,850 + Pf1N1B4_5268 0.44 -0.7 25,850 + Pf1N1B4_5268 0.44 +1.1 25,850 + Pf1N1B4_5268 0.44 -2.3 25,850 + Pf1N1B4_5268 0.44 -0.0 25,979 + Pf1N1B4_5268 0.69 +2.4 25,979 + Pf1N1B4_5268 0.69 -3.2 25,979 + Pf1N1B4_5268 0.69 +1.3 25,989 + Pf1N1B4_5268 0.71 +0.9 25,990 - Pf1N1B4_5268 0.71 +0.1 25,990 - Pf1N1B4_5268 0.71 +0.9 26,054 + Pf1N1B4_5268 0.83 +0.7 26,054 + Pf1N1B4_5268 0.83 +0.3 26,054 + Pf1N1B4_5268 0.83 +0.9 26,055 - Pf1N1B4_5268 0.84 +1.3 26,058 + +2.3 26,058 + Pf1N1B4_5268 0.84 +1.2 26,058 + Pf1N1B4_5268 0.84 +0.9 26,058 + Pf1N1B4_5268 0.84 +0.6 26,058 + Pf1N1B4_5268 0.84 -2.1 26,058 + Pf1N1B4_5268 0.84 +0.7 26,059 - Pf1N1B4_5268 0.84 -0.3 26,059 - Pf1N1B4_5268 0.84 -1.3 26,128 - -0.5 26,128 - -0.1 26,166 + -0.7 26,166 + +0.3 26,167 - -2.1 26,189 + +1.0 26,189 + -0.4 26,190 - -0.5 26,190 - -0.5 26,227 - +0.9 26,348 + +0.3 26,348 + -0.4 26,382 - -1.7 26,385 + +0.9 26,386 - +0.0 26,521 + Pf1N1B4_5269 0.17 +2.2 26,521 + Pf1N1B4_5269 0.17 +0.0 26,521 + Pf1N1B4_5269 0.17 +1.2 26,522 - Pf1N1B4_5269 0.17 -0.3 26,660 + Pf1N1B4_5269 0.64 +2.1 26,660 + Pf1N1B4_5269 0.64 -0.5 26,660 + Pf1N1B4_5269 0.64 -0.9 26,661 - Pf1N1B4_5269 0.65 +0.6 26,837 - -1.1 26,980 - -0.6 26,980 - -0.7 26,980 - +1.1 27,045 + -1.2 27,046 - +1.1 27,046 - +0.3 27,046 - +1.0 27,046 - +2.2 27,046 - +0.1 27,145 + +0.0 27,146 - -0.7 27,181 + +1.4 27,182 - +0.9 27,190 - +1.3 27,190 - -0.6 27,190 - +0.6 27,238 - +1.9 27,269 + +0.2 27,294 - -0.1 27,332 - +0.5 27,365 + -0.5 27,366 - -0.5 27,433 - -0.5 27,493 + Pf1N1B4_5270 0.15 -0.4 27,500 + +1.3 27,594 + Pf1N1B4_5270 0.27 +0.4 27,595 - +2.4 27,613 - Pf1N1B4_5270 0.30 +1.4 27,637 - Pf1N1B4_5270 0.32 +0.3 27,672 + Pf1N1B4_5270 0.37 +0.3 27,693 + -0.7 27,694 - Pf1N1B4_5270 0.39 +2.6 27,694 - Pf1N1B4_5270 0.39 -0.1 27,746 + Pf1N1B4_5270 0.46 +0.9 27,746 + Pf1N1B4_5270 0.46 +0.2 27,747 - Pf1N1B4_5270 0.46 +0.9 27,759 - Pf1N1B4_5270 0.47 -1.7 27,759 - Pf1N1B4_5270 0.47 +0.2
Or see this region's nucleotide sequence