Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5128 and Pf1N1B4_5129 are separated by 408 nucleotides Pf1N1B4_5129 and Pf1N1B4_5130 are separated by 531 nucleotides
Pf1N1B4_5128 - ABC-type protease/lipase transport system, ATPase and permease components, at 5,705,253 to 5,706,014
_5128
Pf1N1B4_5129 - D-galacturonate transporter ExuT (from data) , at 5,706,423 to 5,707,826
_5129
Pf1N1B4_5130 - FIG01220323: hypothetical protein, at 5,708,358 to 5,709,413
_5130
Position (kb)
5706
5707
5708 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5705.432 kb on - strand, within Pf1N1B4_5128 at 5705.432 kb on - strand, within Pf1N1B4_5128 at 5705.581 kb on + strand, within Pf1N1B4_5128 at 5705.581 kb on + strand, within Pf1N1B4_5128 at 5705.581 kb on + strand, within Pf1N1B4_5128 at 5705.582 kb on - strand, within Pf1N1B4_5128 at 5705.582 kb on - strand at 5705.586 kb on - strand, within Pf1N1B4_5128 at 5705.723 kb on - strand, within Pf1N1B4_5128 at 5705.757 kb on - strand, within Pf1N1B4_5128 at 5705.882 kb on - strand, within Pf1N1B4_5128 at 5705.894 kb on + strand, within Pf1N1B4_5128 at 5705.894 kb on + strand, within Pf1N1B4_5128 at 5705.895 kb on - strand at 5705.896 kb on + strand, within Pf1N1B4_5128 at 5705.896 kb on + strand, within Pf1N1B4_5128 at 5705.896 kb on + strand, within Pf1N1B4_5128 at 5705.896 kb on + strand, within Pf1N1B4_5128 at 5705.896 kb on + strand at 5705.897 kb on - strand, within Pf1N1B4_5128 at 5705.897 kb on - strand, within Pf1N1B4_5128 at 5705.897 kb on - strand, within Pf1N1B4_5128 at 5705.897 kb on - strand, within Pf1N1B4_5128 at 5705.897 kb on - strand, within Pf1N1B4_5128 at 5705.897 kb on - strand, within Pf1N1B4_5128 at 5705.954 kb on + strand at 5706.051 kb on + strand at 5706.052 kb on - strand at 5706.158 kb on + strand at 5706.158 kb on + strand at 5706.158 kb on + strand at 5706.158 kb on + strand at 5706.159 kb on - strand at 5706.159 kb on - strand at 5706.327 kb on + strand at 5706.327 kb on + strand at 5706.327 kb on + strand at 5706.328 kb on - strand at 5706.328 kb on - strand at 5706.328 kb on - strand at 5706.328 kb on - strand at 5706.425 kb on - strand at 5706.454 kb on - strand at 5706.521 kb on - strand at 5706.521 kb on - strand at 5706.521 kb on - strand at 5706.749 kb on - strand, within Pf1N1B4_5129 at 5706.749 kb on - strand, within Pf1N1B4_5129 at 5706.850 kb on + strand, within Pf1N1B4_5129 at 5706.898 kb on + strand at 5706.958 kb on + strand, within Pf1N1B4_5129 at 5706.959 kb on - strand, within Pf1N1B4_5129 at 5706.959 kb on - strand, within Pf1N1B4_5129 at 5706.959 kb on - strand, within Pf1N1B4_5129 at 5706.959 kb on - strand, within Pf1N1B4_5129 at 5707.177 kb on + strand, within Pf1N1B4_5129 at 5707.178 kb on - strand, within Pf1N1B4_5129 at 5707.217 kb on + strand, within Pf1N1B4_5129 at 5707.354 kb on + strand at 5707.354 kb on + strand, within Pf1N1B4_5129 at 5707.354 kb on + strand, within Pf1N1B4_5129 at 5707.355 kb on - strand, within Pf1N1B4_5129 at 5707.355 kb on - strand at 5707.554 kb on + strand, within Pf1N1B4_5129 at 5707.555 kb on - strand, within Pf1N1B4_5129 at 5707.555 kb on - strand at 5707.555 kb on - strand, within Pf1N1B4_5129 at 5707.555 kb on - strand, within Pf1N1B4_5129 at 5707.684 kb on - strand, within Pf1N1B4_5129 at 5707.723 kb on + strand at 5707.772 kb on - strand at 5707.837 kb on + strand at 5707.837 kb on + strand at 5707.837 kb on + strand at 5707.838 kb on - strand at 5707.849 kb on - strand at 5707.870 kb on + strand at 5707.889 kb on + strand at 5708.035 kb on + strand at 5708.036 kb on - strand at 5708.105 kb on + strand at 5708.232 kb on - strand at 5708.244 kb on - strand at 5708.244 kb on - strand at 5708.411 kb on - strand at 5708.512 kb on + strand, within Pf1N1B4_5130 at 5708.513 kb on - strand, within Pf1N1B4_5130 at 5708.513 kb on - strand, within Pf1N1B4_5130 at 5708.513 kb on - strand, within Pf1N1B4_5130 at 5708.605 kb on - strand, within Pf1N1B4_5130 at 5708.605 kb on - strand, within Pf1N1B4_5130 at 5708.621 kb on - strand, within Pf1N1B4_5130 at 5708.621 kb on - strand, within Pf1N1B4_5130 at 5708.737 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 5,705,432 - Pf1N1B4_5128 0.23 +0.2 5,705,432 - Pf1N1B4_5128 0.23 +0.8 5,705,581 + Pf1N1B4_5128 0.43 -1.2 5,705,581 + Pf1N1B4_5128 0.43 -1.0 5,705,581 + Pf1N1B4_5128 0.43 -1.7 5,705,582 - Pf1N1B4_5128 0.43 -0.7 5,705,582 - -0.6 5,705,586 - Pf1N1B4_5128 0.44 +0.0 5,705,723 - Pf1N1B4_5128 0.62 -0.4 5,705,757 - Pf1N1B4_5128 0.66 +0.8 5,705,882 - Pf1N1B4_5128 0.83 -1.7 5,705,894 + Pf1N1B4_5128 0.84 -2.4 5,705,894 + Pf1N1B4_5128 0.84 +0.8 5,705,895 - +2.0 5,705,896 + Pf1N1B4_5128 0.84 +1.2 5,705,896 + Pf1N1B4_5128 0.84 +1.5 5,705,896 + Pf1N1B4_5128 0.84 -0.6 5,705,896 + Pf1N1B4_5128 0.84 -0.6 5,705,896 + +1.8 5,705,897 - Pf1N1B4_5128 0.85 -2.2 5,705,897 - Pf1N1B4_5128 0.85 +0.0 5,705,897 - Pf1N1B4_5128 0.85 -0.6 5,705,897 - Pf1N1B4_5128 0.85 +0.1 5,705,897 - Pf1N1B4_5128 0.85 +1.0 5,705,897 - Pf1N1B4_5128 0.85 -1.9 5,705,954 + +0.2 5,706,051 + +0.4 5,706,052 - -1.0 5,706,158 + -0.3 5,706,158 + +0.3 5,706,158 + -1.9 5,706,158 + +1.0 5,706,159 - -0.7 5,706,159 - +0.0 5,706,327 + +0.6 5,706,327 + -1.1 5,706,327 + -1.7 5,706,328 - -2.2 5,706,328 - -0.8 5,706,328 - -1.2 5,706,328 - -2.2 5,706,425 - -0.1 5,706,454 - -0.6 5,706,521 - +1.4 5,706,521 - -0.8 5,706,521 - +0.7 5,706,749 - Pf1N1B4_5129 0.23 -1.4 5,706,749 - Pf1N1B4_5129 0.23 -0.3 5,706,850 + Pf1N1B4_5129 0.30 -0.4 5,706,898 + +0.4 5,706,958 + Pf1N1B4_5129 0.38 -1.6 5,706,959 - Pf1N1B4_5129 0.38 -2.4 5,706,959 - Pf1N1B4_5129 0.38 -2.4 5,706,959 - Pf1N1B4_5129 0.38 -0.6 5,706,959 - Pf1N1B4_5129 0.38 -0.0 5,707,177 + Pf1N1B4_5129 0.54 +0.6 5,707,178 - Pf1N1B4_5129 0.54 -0.2 5,707,217 + Pf1N1B4_5129 0.57 -0.9 5,707,354 + -1.6 5,707,354 + Pf1N1B4_5129 0.66 +0.7 5,707,354 + Pf1N1B4_5129 0.66 +0.1 5,707,355 - Pf1N1B4_5129 0.66 -0.2 5,707,355 - -0.6 5,707,554 + Pf1N1B4_5129 0.81 -0.4 5,707,555 - Pf1N1B4_5129 0.81 -2.2 5,707,555 - -0.2 5,707,555 - Pf1N1B4_5129 0.81 -3.6 5,707,555 - Pf1N1B4_5129 0.81 -2.6 5,707,684 - Pf1N1B4_5129 0.90 +1.4 5,707,723 + +0.1 5,707,772 - +0.8 5,707,837 + -1.6 5,707,837 + +1.7 5,707,837 + +0.9 5,707,838 - -0.6 5,707,849 - +1.1 5,707,870 + +0.4 5,707,889 + -0.6 5,708,035 + +0.0 5,708,036 - -0.9 5,708,105 + +0.0 5,708,232 - -0.2 5,708,244 - -0.6 5,708,244 - -0.2 5,708,411 - -1.0 5,708,512 + Pf1N1B4_5130 0.15 +0.8 5,708,513 - Pf1N1B4_5130 0.15 -0.1 5,708,513 - Pf1N1B4_5130 0.15 -0.9 5,708,513 - Pf1N1B4_5130 0.15 -1.2 5,708,605 - Pf1N1B4_5130 0.23 +0.7 5,708,605 - Pf1N1B4_5130 0.23 -0.3 5,708,621 - Pf1N1B4_5130 0.25 +0.4 5,708,621 - Pf1N1B4_5130 0.25 -0.3 5,708,737 + +0.4
Or see this region's nucleotide sequence