Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4847 and Pf1N1B4_4848 are separated by 26 nucleotides Pf1N1B4_4848 and Pf1N1B4_4849 are separated by 10 nucleotides
Pf1N1B4_4847 - Various polyols ABC transporter, ATP-binding component, at 5,355,866 to 5,356,969
_4847
Pf1N1B4_4848 - Various polyols ABC transporter, permease component 2, at 5,356,996 to 5,357,826
_4848
Pf1N1B4_4849 - Various polyols ABC transporter, permease component 1, at 5,357,837 to 5,358,736
_4849
Position (kb) 5356
5357
5358 Strain fitness (log2 ratio) -2
-1
0
1 at 5356.404 kb on + strand, within Pf1N1B4_4847 at 5356.404 kb on + strand, within Pf1N1B4_4847 at 5356.404 kb on + strand, within Pf1N1B4_4847 at 5356.404 kb on + strand, within Pf1N1B4_4847 at 5356.405 kb on - strand, within Pf1N1B4_4847 at 5356.720 kb on - strand, within Pf1N1B4_4847 at 5356.720 kb on - strand, within Pf1N1B4_4847 at 5356.994 kb on - strand at 5357.342 kb on + strand, within Pf1N1B4_4848 at 5357.343 kb on - strand, within Pf1N1B4_4848 at 5357.343 kb on - strand at 5357.348 kb on + strand, within Pf1N1B4_4848 at 5357.349 kb on - strand, within Pf1N1B4_4848 at 5357.387 kb on + strand, within Pf1N1B4_4848 at 5357.445 kb on - strand, within Pf1N1B4_4848 at 5357.528 kb on + strand, within Pf1N1B4_4848 at 5357.529 kb on - strand, within Pf1N1B4_4848 at 5357.625 kb on - strand at 5357.651 kb on + strand, within Pf1N1B4_4848 at 5357.651 kb on + strand, within Pf1N1B4_4848 at 5357.651 kb on + strand at 5357.652 kb on - strand, within Pf1N1B4_4848 at 5357.976 kb on + strand, within Pf1N1B4_4849 at 5358.306 kb on + strand, within Pf1N1B4_4849 at 5358.307 kb on - strand, within Pf1N1B4_4849 at 5358.712 kb on + strand at 5358.757 kb on + strand at 5358.780 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 5,356,404 + Pf1N1B4_4847 0.49 +0.1 5,356,404 + Pf1N1B4_4847 0.49 -0.1 5,356,404 + Pf1N1B4_4847 0.49 -0.4 5,356,404 + Pf1N1B4_4847 0.49 -0.0 5,356,405 - Pf1N1B4_4847 0.49 -1.6 5,356,720 - Pf1N1B4_4847 0.77 -1.0 5,356,720 - Pf1N1B4_4847 0.77 +0.3 5,356,994 - +1.2 5,357,342 + Pf1N1B4_4848 0.42 +0.8 5,357,343 - Pf1N1B4_4848 0.42 +1.1 5,357,343 - -1.1 5,357,348 + Pf1N1B4_4848 0.42 -1.6 5,357,349 - Pf1N1B4_4848 0.42 +1.6 5,357,387 + Pf1N1B4_4848 0.47 +0.9 5,357,445 - Pf1N1B4_4848 0.54 +0.3 5,357,528 + Pf1N1B4_4848 0.64 +1.4 5,357,529 - Pf1N1B4_4848 0.64 -0.6 5,357,625 - +0.4 5,357,651 + Pf1N1B4_4848 0.79 +0.2 5,357,651 + Pf1N1B4_4848 0.79 +0.4 5,357,651 + -1.1 5,357,652 - Pf1N1B4_4848 0.79 -0.9 5,357,976 + Pf1N1B4_4849 0.15 +0.1 5,358,306 + Pf1N1B4_4849 0.52 +0.2 5,358,307 - Pf1N1B4_4849 0.52 +0.3 5,358,712 + -2.4 5,358,757 + +0.4 5,358,780 - +0.6
Or see this region's nucleotide sequence