Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4770 and Pf1N1B4_4771 are separated by 101 nucleotides Pf1N1B4_4771 and Pf1N1B4_4772 are separated by 190 nucleotides
Pf1N1B4_4770 - Putative autotransporter, at 5,268,195 to 5,270,408
_4770
Pf1N1B4_4771 - Probable transmembrane protein, at 5,270,510 to 5,271,151
_4771
Pf1N1B4_4772 - RND efflux system, inner membrane transporter CmeB, at 5,271,342 to 5,274,440
_4772
Position (kb) 5270
5271
5272 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 5269.642 kb on - strand, within Pf1N1B4_4770 at 5269.947 kb on + strand, within Pf1N1B4_4770 at 5269.947 kb on + strand, within Pf1N1B4_4770 at 5269.947 kb on + strand, within Pf1N1B4_4770 at 5269.947 kb on + strand, within Pf1N1B4_4770 at 5269.948 kb on - strand, within Pf1N1B4_4770 at 5269.991 kb on + strand, within Pf1N1B4_4770 at 5270.010 kb on + strand, within Pf1N1B4_4770 at 5270.010 kb on + strand, within Pf1N1B4_4770 at 5270.029 kb on + strand, within Pf1N1B4_4770 at 5270.029 kb on + strand at 5270.029 kb on + strand, within Pf1N1B4_4770 at 5270.029 kb on + strand, within Pf1N1B4_4770 at 5270.029 kb on + strand, within Pf1N1B4_4770 at 5270.035 kb on + strand, within Pf1N1B4_4770 at 5270.267 kb on + strand at 5270.315 kb on + strand at 5270.315 kb on + strand at 5270.315 kb on + strand at 5270.386 kb on + strand at 5270.386 kb on + strand at 5270.386 kb on + strand at 5270.386 kb on + strand at 5270.397 kb on + strand at 5270.398 kb on - strand at 5270.561 kb on - strand at 5270.561 kb on - strand at 5270.561 kb on - strand at 5270.561 kb on - strand at 5270.561 kb on - strand at 5270.745 kb on + strand, within Pf1N1B4_4771 at 5270.745 kb on + strand at 5270.746 kb on - strand, within Pf1N1B4_4771 at 5270.748 kb on + strand, within Pf1N1B4_4771 at 5270.748 kb on + strand at 5270.749 kb on - strand, within Pf1N1B4_4771 at 5270.749 kb on - strand at 5270.760 kb on + strand, within Pf1N1B4_4771 at 5270.761 kb on - strand, within Pf1N1B4_4771 at 5270.927 kb on + strand at 5270.927 kb on + strand at 5270.927 kb on + strand, within Pf1N1B4_4771 at 5271.177 kb on - strand at 5271.602 kb on - strand at 5271.894 kb on + strand, within Pf1N1B4_4772 at 5272.083 kb on - strand, within Pf1N1B4_4772 at 5272.083 kb on - strand, within Pf1N1B4_4772 at 5272.083 kb on - strand, within Pf1N1B4_4772 at 5272.083 kb on - strand, within Pf1N1B4_4772 at 5272.087 kb on - strand at 5272.087 kb on - strand, within Pf1N1B4_4772
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 5,269,642 - Pf1N1B4_4770 0.65 -0.8 5,269,947 + Pf1N1B4_4770 0.79 +0.2 5,269,947 + Pf1N1B4_4770 0.79 +1.0 5,269,947 + Pf1N1B4_4770 0.79 -1.5 5,269,947 + Pf1N1B4_4770 0.79 -0.3 5,269,948 - Pf1N1B4_4770 0.79 +2.0 5,269,991 + Pf1N1B4_4770 0.81 -0.8 5,270,010 + Pf1N1B4_4770 0.82 +2.0 5,270,010 + Pf1N1B4_4770 0.82 -0.5 5,270,029 + Pf1N1B4_4770 0.83 +0.5 5,270,029 + +1.0 5,270,029 + Pf1N1B4_4770 0.83 +0.0 5,270,029 + Pf1N1B4_4770 0.83 -0.1 5,270,029 + Pf1N1B4_4770 0.83 -1.1 5,270,035 + Pf1N1B4_4770 0.83 -0.1 5,270,267 + -0.4 5,270,315 + -1.1 5,270,315 + +0.7 5,270,315 + -0.5 5,270,386 + +0.6 5,270,386 + -0.7 5,270,386 + +0.9 5,270,386 + +0.5 5,270,397 + -1.1 5,270,398 - +0.9 5,270,561 - -1.9 5,270,561 - -1.3 5,270,561 - +0.7 5,270,561 - +0.5 5,270,561 - -0.5 5,270,745 + Pf1N1B4_4771 0.37 -2.9 5,270,745 + -0.5 5,270,746 - Pf1N1B4_4771 0.37 +1.1 5,270,748 + Pf1N1B4_4771 0.37 -1.5 5,270,748 + +0.5 5,270,749 - Pf1N1B4_4771 0.37 -0.1 5,270,749 - +0.5 5,270,760 + Pf1N1B4_4771 0.39 -1.1 5,270,761 - Pf1N1B4_4771 0.39 -2.7 5,270,927 + -0.5 5,270,927 + +0.5 5,270,927 + Pf1N1B4_4771 0.65 -1.2 5,271,177 - -0.3 5,271,602 - -2.5 5,271,894 + Pf1N1B4_4772 0.18 -2.3 5,272,083 - Pf1N1B4_4772 0.24 -5.0 5,272,083 - Pf1N1B4_4772 0.24 -1.1 5,272,083 - Pf1N1B4_4772 0.24 -1.1 5,272,083 - Pf1N1B4_4772 0.24 -2.7 5,272,087 - -0.5 5,272,087 - Pf1N1B4_4772 0.24 +0.5
Or see this region's nucleotide sequence