Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4720 and Pf1N1B4_4721 are separated by 18 nucleotides Pf1N1B4_4721 and Pf1N1B4_4722 are separated by 116 nucleotides Pf1N1B4_4722 and Pf1N1B4_4723 are separated by 194 nucleotides
Pf1N1B4_4720 - AFG1-like ATPase, at 5,216,261 to 5,217,379
_4720
Pf1N1B4_4721 - Nuclease inhibitor, at 5,217,398 to 5,217,979
_4721
Pf1N1B4_4722 - Histone acetyltransferase HPA2 and related acetyltransferases, at 5,218,096 to 5,218,587
_4722
Pf1N1B4_4723 - UPF0141 membrane protein YijP possibly required for phosphoethanolamine modification of lipopolysaccharide, at 5,218,782 to 5,220,527
_4723
Position (kb) 5217
5218 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5216.409 kb on - strand, within Pf1N1B4_4720 at 5216.409 kb on - strand, within Pf1N1B4_4720 at 5216.409 kb on - strand at 5216.725 kb on - strand, within Pf1N1B4_4720 at 5217.261 kb on - strand, within Pf1N1B4_4720 at 5217.294 kb on - strand at 5217.306 kb on - strand at 5217.576 kb on - strand at 5217.576 kb on - strand, within Pf1N1B4_4721 at 5217.576 kb on - strand, within Pf1N1B4_4721 at 5217.703 kb on + strand, within Pf1N1B4_4721 at 5218.132 kb on + strand at 5218.169 kb on + strand at 5218.224 kb on + strand, within Pf1N1B4_4722 at 5218.489 kb on - strand, within Pf1N1B4_4722 at 5218.698 kb on + strand at 5218.751 kb on + strand at 5218.751 kb on + strand at 5218.783 kb on + strand at 5218.809 kb on - strand at 5218.868 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 5,216,409 - Pf1N1B4_4720 0.13 +1.8 5,216,409 - Pf1N1B4_4720 0.13 +1.1 5,216,409 - +0.9 5,216,725 - Pf1N1B4_4720 0.41 +0.3 5,217,261 - Pf1N1B4_4720 0.89 -0.3 5,217,294 - +0.0 5,217,306 - -0.5 5,217,576 - -0.5 5,217,576 - Pf1N1B4_4721 0.31 -2.3 5,217,576 - Pf1N1B4_4721 0.31 -0.8 5,217,703 + Pf1N1B4_4721 0.52 +0.2 5,218,132 + -1.1 5,218,169 + -1.1 5,218,224 + Pf1N1B4_4722 0.26 +0.2 5,218,489 - Pf1N1B4_4722 0.80 -0.5 5,218,698 + -1.9 5,218,751 + -0.5 5,218,751 + +0.4 5,218,783 + -0.1 5,218,809 - +0.2 5,218,868 - -0.5
Or see this region's nucleotide sequence