Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4715 and Pf1N1B4_4716 are separated by 101 nucleotides Pf1N1B4_4716 and Pf1N1B4_4717 are separated by 21 nucleotides
Pf1N1B4_4715 - Transcriptional regulator, at 5,211,729 to 5,212,622
_4715
Pf1N1B4_4716 - Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2), at 5,212,724 to 5,213,815
_4716
Pf1N1B4_4717 - Agmatinase (EC 3.5.3.11), at 5,213,837 to 5,214,796
_4717
Position (kb)
5212
5213
5214 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5211.727 kb on - strand at 5211.727 kb on - strand at 5211.727 kb on - strand at 5212.091 kb on - strand, within Pf1N1B4_4715 at 5212.154 kb on - strand, within Pf1N1B4_4715 at 5212.154 kb on - strand, within Pf1N1B4_4715 at 5212.652 kb on - strand at 5212.811 kb on + strand at 5212.812 kb on - strand at 5212.883 kb on + strand at 5213.105 kb on + strand, within Pf1N1B4_4716 at 5213.105 kb on + strand, within Pf1N1B4_4716 at 5213.207 kb on + strand, within Pf1N1B4_4716 at 5213.282 kb on + strand, within Pf1N1B4_4716 at 5213.283 kb on - strand, within Pf1N1B4_4716 at 5213.352 kb on + strand, within Pf1N1B4_4716 at 5213.432 kb on + strand, within Pf1N1B4_4716 at 5213.629 kb on + strand at 5213.708 kb on + strand at 5213.831 kb on + strand at 5213.831 kb on + strand at 5213.831 kb on + strand at 5213.832 kb on - strand at 5213.832 kb on - strand at 5213.832 kb on - strand at 5213.909 kb on + strand at 5213.909 kb on + strand at 5213.909 kb on + strand at 5213.910 kb on - strand at 5213.910 kb on - strand at 5214.545 kb on + strand, within Pf1N1B4_4717 at 5214.721 kb on + strand at 5214.721 kb on + strand at 5214.722 kb on - strand at 5214.767 kb on + strand at 5214.767 kb on + strand at 5214.768 kb on - strand at 5214.768 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 5,211,727 - -0.3 5,211,727 - -1.5 5,211,727 - +2.1 5,212,091 - Pf1N1B4_4715 0.40 -0.5 5,212,154 - Pf1N1B4_4715 0.48 +0.1 5,212,154 - Pf1N1B4_4715 0.48 +1.2 5,212,652 - +1.1 5,212,811 + -1.6 5,212,812 - +0.6 5,212,883 + +1.1 5,213,105 + Pf1N1B4_4716 0.35 -0.1 5,213,105 + Pf1N1B4_4716 0.35 -0.4 5,213,207 + Pf1N1B4_4716 0.44 -0.5 5,213,282 + Pf1N1B4_4716 0.51 -2.3 5,213,283 - Pf1N1B4_4716 0.51 -1.1 5,213,352 + Pf1N1B4_4716 0.58 +0.9 5,213,432 + Pf1N1B4_4716 0.65 -0.3 5,213,629 + -0.5 5,213,708 + +0.0 5,213,831 + -0.5 5,213,831 + -2.3 5,213,831 + -0.1 5,213,832 - +1.5 5,213,832 - +2.5 5,213,832 - -0.3 5,213,909 + -0.5 5,213,909 + +1.5 5,213,909 + +0.5 5,213,910 - -0.5 5,213,910 - +0.5 5,214,545 + Pf1N1B4_4717 0.74 +1.2 5,214,721 + -0.5 5,214,721 + +1.1 5,214,722 - -0.2 5,214,767 + +0.5 5,214,767 + +0.5 5,214,768 - +0.8 5,214,768 - -0.3
Or see this region's nucleotide sequence