Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4666

Experiment: Spectinomycin 0.05 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4665 and Pf1N1B4_4666 overlap by 4 nucleotidesPf1N1B4_4666 and Pf1N1B4_4667 overlap by 22 nucleotides Pf1N1B4_4665 - Acriflavin resistance plasma membrane protein, at 5,141,918 to 5,145,022 _4665 Pf1N1B4_4666 - Acriflavin resistance plasma membrane protein, at 5,145,019 to 5,146,605 _4666 Pf1N1B4_4667 - Acriflavin resistance plasma membrane protein, at 5,146,584 to 5,148,125 _4667 Position (kb) 5145 5146 5147Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5144.049 kb on - strand, within Pf1N1B4_4665at 5144.049 kb on - strandat 5144.049 kb on - strand, within Pf1N1B4_4665at 5144.118 kb on - strandat 5144.153 kb on + strand, within Pf1N1B4_4665at 5144.154 kb on - strandat 5144.154 kb on - strand, within Pf1N1B4_4665at 5144.154 kb on - strand, within Pf1N1B4_4665at 5144.525 kb on + strand, within Pf1N1B4_4665at 5144.525 kb on + strand, within Pf1N1B4_4665at 5144.525 kb on + strand, within Pf1N1B4_4665at 5144.526 kb on - strand, within Pf1N1B4_4665at 5144.526 kb on - strand, within Pf1N1B4_4665at 5144.526 kb on - strand, within Pf1N1B4_4665at 5144.526 kb on - strand, within Pf1N1B4_4665at 5144.526 kb on - strand, within Pf1N1B4_4665at 5144.555 kb on + strand, within Pf1N1B4_4665at 5144.556 kb on - strand, within Pf1N1B4_4665at 5144.765 kb on + strandat 5144.945 kb on - strandat 5144.948 kb on + strandat 5144.949 kb on - strandat 5144.949 kb on - strandat 5145.403 kb on + strand, within Pf1N1B4_4666at 5145.403 kb on + strand, within Pf1N1B4_4666at 5145.629 kb on + strand, within Pf1N1B4_4666at 5145.630 kb on - strand, within Pf1N1B4_4666at 5145.739 kb on + strand, within Pf1N1B4_4666at 5145.826 kb on + strand, within Pf1N1B4_4666at 5146.150 kb on + strand, within Pf1N1B4_4666at 5146.151 kb on - strand, within Pf1N1B4_4666at 5146.563 kb on + strandat 5146.563 kb on + strandat 5146.564 kb on - strandat 5146.589 kb on - strandat 5147.010 kb on + strand, within Pf1N1B4_4667at 5147.016 kb on + strandat 5147.016 kb on + strandat 5147.248 kb on - strand, within Pf1N1B4_4667

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml
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5,144,049 - Pf1N1B4_4665 0.69 +0.1
5,144,049 - +0.5
5,144,049 - Pf1N1B4_4665 0.69 -0.5
5,144,118 - -1.1
5,144,153 + Pf1N1B4_4665 0.72 -0.5
5,144,154 - -0.1
5,144,154 - Pf1N1B4_4665 0.72 +0.8
5,144,154 - Pf1N1B4_4665 0.72 -1.1
5,144,525 + Pf1N1B4_4665 0.84 +1.9
5,144,525 + Pf1N1B4_4665 0.84 +1.9
5,144,525 + Pf1N1B4_4665 0.84 +0.5
5,144,526 - Pf1N1B4_4665 0.84 +0.8
5,144,526 - Pf1N1B4_4665 0.84 +0.1
5,144,526 - Pf1N1B4_4665 0.84 +0.5
5,144,526 - Pf1N1B4_4665 0.84 +2.1
5,144,526 - Pf1N1B4_4665 0.84 +0.5
5,144,555 + Pf1N1B4_4665 0.85 -2.8
5,144,556 - Pf1N1B4_4665 0.85 -0.7
5,144,765 + -1.1
5,144,945 - +0.5
5,144,948 + +1.1
5,144,949 - -1.1
5,144,949 - +0.8
5,145,403 + Pf1N1B4_4666 0.24 +1.1
5,145,403 + Pf1N1B4_4666 0.24 -1.5
5,145,629 + Pf1N1B4_4666 0.38 -2.1
5,145,630 - Pf1N1B4_4666 0.39 -1.1
5,145,739 + Pf1N1B4_4666 0.45 +1.5
5,145,826 + Pf1N1B4_4666 0.51 -1.3
5,146,150 + Pf1N1B4_4666 0.71 +0.5
5,146,151 - Pf1N1B4_4666 0.71 -1.0
5,146,563 + -0.7
5,146,563 + -0.1
5,146,564 - -1.1
5,146,589 - -2.7
5,147,010 + Pf1N1B4_4667 0.28 -2.1
5,147,016 + +0.5
5,147,016 + -1.1
5,147,248 - Pf1N1B4_4667 0.43 -0.8

Or see this region's nucleotide sequence