Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4665 and Pf1N1B4_4666 overlap by 4 nucleotides Pf1N1B4_4666 and Pf1N1B4_4667 overlap by 22 nucleotides
Pf1N1B4_4665 - Acriflavin resistance plasma membrane protein, at 5,141,918 to 5,145,022
_4665
Pf1N1B4_4666 - Acriflavin resistance plasma membrane protein, at 5,145,019 to 5,146,605
_4666
Pf1N1B4_4667 - Acriflavin resistance plasma membrane protein, at 5,146,584 to 5,148,125
_4667
Position (kb)
5145
5146
5147 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5144.049 kb on - strand, within Pf1N1B4_4665 at 5144.049 kb on - strand at 5144.049 kb on - strand, within Pf1N1B4_4665 at 5144.118 kb on - strand at 5144.153 kb on + strand, within Pf1N1B4_4665 at 5144.154 kb on - strand at 5144.154 kb on - strand, within Pf1N1B4_4665 at 5144.154 kb on - strand, within Pf1N1B4_4665 at 5144.525 kb on + strand, within Pf1N1B4_4665 at 5144.525 kb on + strand, within Pf1N1B4_4665 at 5144.525 kb on + strand, within Pf1N1B4_4665 at 5144.526 kb on - strand, within Pf1N1B4_4665 at 5144.526 kb on - strand, within Pf1N1B4_4665 at 5144.526 kb on - strand, within Pf1N1B4_4665 at 5144.526 kb on - strand, within Pf1N1B4_4665 at 5144.526 kb on - strand, within Pf1N1B4_4665 at 5144.555 kb on + strand, within Pf1N1B4_4665 at 5144.556 kb on - strand, within Pf1N1B4_4665 at 5144.765 kb on + strand at 5144.945 kb on - strand at 5144.948 kb on + strand at 5144.949 kb on - strand at 5144.949 kb on - strand at 5145.403 kb on + strand, within Pf1N1B4_4666 at 5145.403 kb on + strand, within Pf1N1B4_4666 at 5145.629 kb on + strand, within Pf1N1B4_4666 at 5145.630 kb on - strand, within Pf1N1B4_4666 at 5145.739 kb on + strand, within Pf1N1B4_4666 at 5145.826 kb on + strand, within Pf1N1B4_4666 at 5146.150 kb on + strand, within Pf1N1B4_4666 at 5146.151 kb on - strand, within Pf1N1B4_4666 at 5146.563 kb on + strand at 5146.563 kb on + strand at 5146.564 kb on - strand at 5146.589 kb on - strand at 5147.010 kb on + strand, within Pf1N1B4_4667 at 5147.016 kb on + strand at 5147.016 kb on + strand at 5147.248 kb on - strand, within Pf1N1B4_4667
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 5,144,049 - Pf1N1B4_4665 0.69 +0.1 5,144,049 - +0.5 5,144,049 - Pf1N1B4_4665 0.69 -0.5 5,144,118 - -1.1 5,144,153 + Pf1N1B4_4665 0.72 -0.5 5,144,154 - -0.1 5,144,154 - Pf1N1B4_4665 0.72 +0.8 5,144,154 - Pf1N1B4_4665 0.72 -1.1 5,144,525 + Pf1N1B4_4665 0.84 +1.9 5,144,525 + Pf1N1B4_4665 0.84 +1.9 5,144,525 + Pf1N1B4_4665 0.84 +0.5 5,144,526 - Pf1N1B4_4665 0.84 +0.8 5,144,526 - Pf1N1B4_4665 0.84 +0.1 5,144,526 - Pf1N1B4_4665 0.84 +0.5 5,144,526 - Pf1N1B4_4665 0.84 +2.1 5,144,526 - Pf1N1B4_4665 0.84 +0.5 5,144,555 + Pf1N1B4_4665 0.85 -2.8 5,144,556 - Pf1N1B4_4665 0.85 -0.7 5,144,765 + -1.1 5,144,945 - +0.5 5,144,948 + +1.1 5,144,949 - -1.1 5,144,949 - +0.8 5,145,403 + Pf1N1B4_4666 0.24 +1.1 5,145,403 + Pf1N1B4_4666 0.24 -1.5 5,145,629 + Pf1N1B4_4666 0.38 -2.1 5,145,630 - Pf1N1B4_4666 0.39 -1.1 5,145,739 + Pf1N1B4_4666 0.45 +1.5 5,145,826 + Pf1N1B4_4666 0.51 -1.3 5,146,150 + Pf1N1B4_4666 0.71 +0.5 5,146,151 - Pf1N1B4_4666 0.71 -1.0 5,146,563 + -0.7 5,146,563 + -0.1 5,146,564 - -1.1 5,146,589 - -2.7 5,147,010 + Pf1N1B4_4667 0.28 -2.1 5,147,016 + +0.5 5,147,016 + -1.1 5,147,248 - Pf1N1B4_4667 0.43 -0.8
Or see this region's nucleotide sequence