Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4502

Experiment: Spectinomycin 0.05 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4501 and Pf1N1B4_4502 are separated by 240 nucleotidesPf1N1B4_4502 and Pf1N1B4_4503 are separated by 385 nucleotides Pf1N1B4_4501 - Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8), at 4,961,625 to 4,963,400 _4501 Pf1N1B4_4502 - Aldehyde dehydrogenase (EC 1.2.1.3), at 4,963,641 to 4,965,161 _4502 Pf1N1B4_4503 - Coenzyme PQQ synthesis protein B, at 4,965,547 to 4,966,461 _4503 Position (kb) 4963 4964 4965 4966Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4962.687 kb on + strand, within Pf1N1B4_4501at 4962.787 kb on - strandat 4962.787 kb on - strand, within Pf1N1B4_4501at 4962.862 kb on + strand, within Pf1N1B4_4501at 4962.915 kb on + strandat 4962.915 kb on + strand, within Pf1N1B4_4501at 4962.915 kb on + strand, within Pf1N1B4_4501at 4962.915 kb on + strand, within Pf1N1B4_4501at 4962.915 kb on + strand, within Pf1N1B4_4501at 4962.915 kb on + strand, within Pf1N1B4_4501at 4962.916 kb on - strandat 4962.916 kb on - strand, within Pf1N1B4_4501at 4962.919 kb on + strand, within Pf1N1B4_4501at 4962.919 kb on + strand, within Pf1N1B4_4501at 4962.966 kb on + strand, within Pf1N1B4_4501at 4963.026 kb on + strand, within Pf1N1B4_4501at 4963.027 kb on - strand, within Pf1N1B4_4501at 4963.263 kb on + strandat 4963.264 kb on - strandat 4963.523 kb on + strandat 4963.523 kb on + strandat 4963.524 kb on - strandat 4963.584 kb on + strandat 4963.584 kb on + strandat 4963.584 kb on + strandat 4963.585 kb on - strandat 4963.647 kb on + strandat 4963.648 kb on - strandat 4964.822 kb on + strand, within Pf1N1B4_4502at 4964.823 kb on - strand, within Pf1N1B4_4502at 4965.232 kb on + strandat 4965.233 kb on - strandat 4965.290 kb on + strandat 4965.347 kb on - strandat 4965.475 kb on + strandat 4965.475 kb on + strandat 4965.476 kb on - strandat 4965.476 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml
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4,962,687 + Pf1N1B4_4501 0.60 -0.2
4,962,787 - +1.5
4,962,787 - Pf1N1B4_4501 0.65 -1.7
4,962,862 + Pf1N1B4_4501 0.70 +2.5
4,962,915 + +0.5
4,962,915 + Pf1N1B4_4501 0.73 -0.5
4,962,915 + Pf1N1B4_4501 0.73 +1.1
4,962,915 + Pf1N1B4_4501 0.73 +1.2
4,962,915 + Pf1N1B4_4501 0.73 -0.7
4,962,915 + Pf1N1B4_4501 0.73 +0.7
4,962,916 - +0.5
4,962,916 - Pf1N1B4_4501 0.73 +0.5
4,962,919 + Pf1N1B4_4501 0.73 -0.2
4,962,919 + Pf1N1B4_4501 0.73 +0.2
4,962,966 + Pf1N1B4_4501 0.76 -0.3
4,963,026 + Pf1N1B4_4501 0.79 +0.9
4,963,027 - Pf1N1B4_4501 0.79 +0.5
4,963,263 + +0.1
4,963,264 - +1.5
4,963,523 + -2.8
4,963,523 + +0.5
4,963,524 - +0.5
4,963,584 + +0.5
4,963,584 + +0.5
4,963,584 + -1.8
4,963,585 - +1.8
4,963,647 + -0.1
4,963,648 - -0.6
4,964,822 + Pf1N1B4_4502 0.78 +0.1
4,964,823 - Pf1N1B4_4502 0.78 +0.8
4,965,232 + -2.7
4,965,233 - -1.7
4,965,290 + -0.2
4,965,347 - -0.5
4,965,475 + +0.5
4,965,475 + -0.5
4,965,476 - -0.1
4,965,476 - -0.4

Or see this region's nucleotide sequence