Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4501 and Pf1N1B4_4502 are separated by 240 nucleotides Pf1N1B4_4502 and Pf1N1B4_4503 are separated by 385 nucleotides
Pf1N1B4_4501 - Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8), at 4,961,625 to 4,963,400
_4501
Pf1N1B4_4502 - Aldehyde dehydrogenase (EC 1.2.1.3), at 4,963,641 to 4,965,161
_4502
Pf1N1B4_4503 - Coenzyme PQQ synthesis protein B, at 4,965,547 to 4,966,461
_4503
Position (kb)
4963
4964
4965
4966 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4962.687 kb on + strand, within Pf1N1B4_4501 at 4962.787 kb on - strand at 4962.787 kb on - strand, within Pf1N1B4_4501 at 4962.862 kb on + strand, within Pf1N1B4_4501 at 4962.915 kb on + strand at 4962.915 kb on + strand, within Pf1N1B4_4501 at 4962.915 kb on + strand, within Pf1N1B4_4501 at 4962.915 kb on + strand, within Pf1N1B4_4501 at 4962.915 kb on + strand, within Pf1N1B4_4501 at 4962.915 kb on + strand, within Pf1N1B4_4501 at 4962.916 kb on - strand at 4962.916 kb on - strand, within Pf1N1B4_4501 at 4962.919 kb on + strand, within Pf1N1B4_4501 at 4962.919 kb on + strand, within Pf1N1B4_4501 at 4962.966 kb on + strand, within Pf1N1B4_4501 at 4963.026 kb on + strand, within Pf1N1B4_4501 at 4963.027 kb on - strand, within Pf1N1B4_4501 at 4963.263 kb on + strand at 4963.264 kb on - strand at 4963.523 kb on + strand at 4963.523 kb on + strand at 4963.524 kb on - strand at 4963.584 kb on + strand at 4963.584 kb on + strand at 4963.584 kb on + strand at 4963.585 kb on - strand at 4963.647 kb on + strand at 4963.648 kb on - strand at 4964.822 kb on + strand, within Pf1N1B4_4502 at 4964.823 kb on - strand, within Pf1N1B4_4502 at 4965.232 kb on + strand at 4965.233 kb on - strand at 4965.290 kb on + strand at 4965.347 kb on - strand at 4965.475 kb on + strand at 4965.475 kb on + strand at 4965.476 kb on - strand at 4965.476 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 4,962,687 + Pf1N1B4_4501 0.60 -0.2 4,962,787 - +1.5 4,962,787 - Pf1N1B4_4501 0.65 -1.7 4,962,862 + Pf1N1B4_4501 0.70 +2.5 4,962,915 + +0.5 4,962,915 + Pf1N1B4_4501 0.73 -0.5 4,962,915 + Pf1N1B4_4501 0.73 +1.1 4,962,915 + Pf1N1B4_4501 0.73 +1.2 4,962,915 + Pf1N1B4_4501 0.73 -0.7 4,962,915 + Pf1N1B4_4501 0.73 +0.7 4,962,916 - +0.5 4,962,916 - Pf1N1B4_4501 0.73 +0.5 4,962,919 + Pf1N1B4_4501 0.73 -0.2 4,962,919 + Pf1N1B4_4501 0.73 +0.2 4,962,966 + Pf1N1B4_4501 0.76 -0.3 4,963,026 + Pf1N1B4_4501 0.79 +0.9 4,963,027 - Pf1N1B4_4501 0.79 +0.5 4,963,263 + +0.1 4,963,264 - +1.5 4,963,523 + -2.8 4,963,523 + +0.5 4,963,524 - +0.5 4,963,584 + +0.5 4,963,584 + +0.5 4,963,584 + -1.8 4,963,585 - +1.8 4,963,647 + -0.1 4,963,648 - -0.6 4,964,822 + Pf1N1B4_4502 0.78 +0.1 4,964,823 - Pf1N1B4_4502 0.78 +0.8 4,965,232 + -2.7 4,965,233 - -1.7 4,965,290 + -0.2 4,965,347 - -0.5 4,965,475 + +0.5 4,965,475 + -0.5 4,965,476 - -0.1 4,965,476 - -0.4
Or see this region's nucleotide sequence