Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4489 and Pf1N1B4_4490 are separated by 57 nucleotides Pf1N1B4_4490 and Pf1N1B4_4491 are separated by 267 nucleotides Pf1N1B4_4491 and Pf1N1B4_4492 overlap by 4 nucleotides Pf1N1B4_4492 and Pf1N1B4_4493 overlap by 4 nucleotides
Pf1N1B4_4489 - Transcriptional regulator, LuxR family, at 4,952,674 to 4,953,339
_4489
Pf1N1B4_4490 - Rhodanese-related sulfurtransferase, at 4,953,397 to 4,953,954
_4490
Pf1N1B4_4491 - ABC transporter permease protein, at 4,954,222 to 4,955,013
_4491
Pf1N1B4_4492 - ABC transporter, ATP-binding protein, at 4,955,010 to 4,955,741
_4492
Pf1N1B4_4493 - FIG00443700: hypothetical protein, at 4,955,738 to 4,956,697
_4493
Position (kb)
4954
4955
4956 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4953.376 kb on + strand at 4953.486 kb on + strand, within Pf1N1B4_4490 at 4953.487 kb on - strand, within Pf1N1B4_4490 at 4953.487 kb on - strand, within Pf1N1B4_4490 at 4953.493 kb on + strand, within Pf1N1B4_4490 at 4953.970 kb on + strand at 4953.970 kb on + strand at 4953.971 kb on - strand at 4953.971 kb on - strand at 4953.971 kb on - strand at 4953.971 kb on - strand at 4953.975 kb on - strand at 4954.028 kb on + strand at 4954.036 kb on + strand at 4954.039 kb on + strand at 4954.039 kb on + strand at 4954.040 kb on - strand at 4954.044 kb on + strand at 4954.099 kb on + strand at 4954.099 kb on + strand at 4954.224 kb on - strand at 4954.614 kb on - strand, within Pf1N1B4_4491 at 4954.614 kb on - strand, within Pf1N1B4_4491 at 4954.640 kb on + strand, within Pf1N1B4_4491 at 4954.640 kb on + strand, within Pf1N1B4_4491 at 4954.641 kb on - strand, within Pf1N1B4_4491 at 4954.752 kb on - strand at 4954.820 kb on + strand, within Pf1N1B4_4491 at 4954.821 kb on - strand, within Pf1N1B4_4491 at 4954.838 kb on + strand, within Pf1N1B4_4491 at 4954.838 kb on + strand, within Pf1N1B4_4491 at 4954.839 kb on - strand at 4955.343 kb on - strand, within Pf1N1B4_4492 at 4955.873 kb on - strand, within Pf1N1B4_4493 at 4955.982 kb on + strand, within Pf1N1B4_4493
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 4,953,376 + +0.9 4,953,486 + Pf1N1B4_4490 0.16 -1.1 4,953,487 - Pf1N1B4_4490 0.16 -2.8 4,953,487 - Pf1N1B4_4490 0.16 +0.7 4,953,493 + Pf1N1B4_4490 0.17 +0.7 4,953,970 + +0.1 4,953,970 + +0.8 4,953,971 - +0.5 4,953,971 - +1.0 4,953,971 - +1.7 4,953,971 - -0.2 4,953,975 - +0.8 4,954,028 + +0.0 4,954,036 + -1.3 4,954,039 + +0.2 4,954,039 + +0.1 4,954,040 - +0.5 4,954,044 + +2.5 4,954,099 + +0.1 4,954,099 + +0.9 4,954,224 - -1.8 4,954,614 - Pf1N1B4_4491 0.49 -0.1 4,954,614 - Pf1N1B4_4491 0.49 -0.2 4,954,640 + Pf1N1B4_4491 0.53 +0.8 4,954,640 + Pf1N1B4_4491 0.53 -1.2 4,954,641 - Pf1N1B4_4491 0.53 -0.7 4,954,752 - -1.1 4,954,820 + Pf1N1B4_4491 0.76 -1.3 4,954,821 - Pf1N1B4_4491 0.76 -0.9 4,954,838 + Pf1N1B4_4491 0.78 -2.9 4,954,838 + Pf1N1B4_4491 0.78 -1.5 4,954,839 - +0.9 4,955,343 - Pf1N1B4_4492 0.45 -1.8 4,955,873 - Pf1N1B4_4493 0.14 +0.7 4,955,982 + Pf1N1B4_4493 0.25 -0.2
Or see this region's nucleotide sequence