Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4366 and Pf1N1B4_4367 are separated by 632 nucleotides Pf1N1B4_4367 and Pf1N1B4_4368 are separated by 15 nucleotides Pf1N1B4_4368 and Pf1N1B4_4369 are separated by 155 nucleotides
Pf1N1B4_4366 - hypothetical protein, at 4,824,247 to 4,824,504
_4366
Pf1N1B4_4367 - hypothetical protein, at 4,825,137 to 4,825,487
_4367
Pf1N1B4_4368 - Guanosine-3',5'-bis(Diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2), at 4,825,503 to 4,825,925
_4368
Pf1N1B4_4369 - hypothetical protein, at 4,826,081 to 4,827,409
_4369
Position (kb)
4825
4826 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4824.608 kb on + strand at 4824.608 kb on + strand at 4824.608 kb on + strand at 4824.609 kb on - strand at 4824.679 kb on + strand at 4824.680 kb on - strand at 4824.715 kb on + strand at 4824.715 kb on + strand at 4824.716 kb on - strand at 4824.716 kb on - strand at 4824.737 kb on - strand at 4824.737 kb on - strand at 4824.744 kb on - strand at 4824.792 kb on + strand at 4824.793 kb on - strand at 4824.871 kb on + strand at 4824.871 kb on + strand at 4824.871 kb on + strand at 4824.977 kb on + strand at 4824.978 kb on - strand at 4824.978 kb on - strand at 4824.978 kb on - strand at 4824.978 kb on - strand at 4825.063 kb on + strand at 4825.197 kb on + strand, within Pf1N1B4_4367 at 4825.197 kb on + strand, within Pf1N1B4_4367 at 4825.581 kb on + strand, within Pf1N1B4_4368 at 4825.581 kb on + strand, within Pf1N1B4_4368 at 4825.583 kb on + strand, within Pf1N1B4_4368 at 4825.583 kb on + strand at 4825.583 kb on + strand, within Pf1N1B4_4368 at 4825.583 kb on + strand at 4825.584 kb on - strand, within Pf1N1B4_4368 at 4825.584 kb on - strand, within Pf1N1B4_4368 at 4825.584 kb on - strand, within Pf1N1B4_4368 at 4825.584 kb on - strand, within Pf1N1B4_4368 at 4825.648 kb on + strand, within Pf1N1B4_4368 at 4825.649 kb on - strand, within Pf1N1B4_4368 at 4825.649 kb on - strand, within Pf1N1B4_4368 at 4826.022 kb on + strand at 4826.122 kb on + strand at 4826.283 kb on - strand, within Pf1N1B4_4369 at 4826.283 kb on - strand, within Pf1N1B4_4369 at 4826.285 kb on - strand, within Pf1N1B4_4369 at 4826.285 kb on - strand, within Pf1N1B4_4369 at 4826.348 kb on + strand, within Pf1N1B4_4369 at 4826.348 kb on + strand, within Pf1N1B4_4369 at 4826.348 kb on + strand, within Pf1N1B4_4369 at 4826.382 kb on + strand, within Pf1N1B4_4369 at 4826.382 kb on + strand, within Pf1N1B4_4369 at 4826.383 kb on - strand, within Pf1N1B4_4369 at 4826.588 kb on + strand, within Pf1N1B4_4369 at 4826.588 kb on + strand, within Pf1N1B4_4369 at 4826.589 kb on - strand, within Pf1N1B4_4369 at 4826.589 kb on - strand, within Pf1N1B4_4369 at 4826.648 kb on + strand, within Pf1N1B4_4369 at 4826.649 kb on - strand, within Pf1N1B4_4369 at 4826.796 kb on - strand, within Pf1N1B4_4369
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 4,824,608 + -0.8 4,824,608 + +0.0 4,824,608 + -0.2 4,824,609 - -0.5 4,824,679 + +0.2 4,824,680 - -0.5 4,824,715 + +0.5 4,824,715 + +0.1 4,824,716 - -0.8 4,824,716 - +0.9 4,824,737 - +0.3 4,824,737 - -3.1 4,824,744 - +0.5 4,824,792 + -0.8 4,824,793 - -1.1 4,824,871 + +0.5 4,824,871 + -0.1 4,824,871 + -0.5 4,824,977 + +1.0 4,824,978 - +0.5 4,824,978 - -0.5 4,824,978 - -1.5 4,824,978 - -0.8 4,825,063 + +1.1 4,825,197 + Pf1N1B4_4367 0.17 +0.5 4,825,197 + Pf1N1B4_4367 0.17 -0.2 4,825,581 + Pf1N1B4_4368 0.18 +0.5 4,825,581 + Pf1N1B4_4368 0.18 -1.8 4,825,583 + Pf1N1B4_4368 0.19 -1.1 4,825,583 + +0.5 4,825,583 + Pf1N1B4_4368 0.19 -0.5 4,825,583 + +0.5 4,825,584 - Pf1N1B4_4368 0.19 -1.3 4,825,584 - Pf1N1B4_4368 0.19 -0.1 4,825,584 - Pf1N1B4_4368 0.19 -1.8 4,825,584 - Pf1N1B4_4368 0.19 +0.8 4,825,648 + Pf1N1B4_4368 0.34 -1.1 4,825,649 - Pf1N1B4_4368 0.35 -0.3 4,825,649 - Pf1N1B4_4368 0.35 +0.8 4,826,022 + +0.1 4,826,122 + +2.3 4,826,283 - Pf1N1B4_4369 0.15 +0.1 4,826,283 - Pf1N1B4_4369 0.15 +1.5 4,826,285 - Pf1N1B4_4369 0.15 -0.5 4,826,285 - Pf1N1B4_4369 0.15 +0.7 4,826,348 + Pf1N1B4_4369 0.20 +0.7 4,826,348 + Pf1N1B4_4369 0.20 +0.3 4,826,348 + Pf1N1B4_4369 0.20 +0.3 4,826,382 + Pf1N1B4_4369 0.23 -0.6 4,826,382 + Pf1N1B4_4369 0.23 +1.1 4,826,383 - Pf1N1B4_4369 0.23 +0.3 4,826,588 + Pf1N1B4_4369 0.38 +1.3 4,826,588 + Pf1N1B4_4369 0.38 -1.8 4,826,589 - Pf1N1B4_4369 0.38 -2.5 4,826,589 - Pf1N1B4_4369 0.38 -0.1 4,826,648 + Pf1N1B4_4369 0.43 -1.2 4,826,649 - Pf1N1B4_4369 0.43 +0.5 4,826,796 - Pf1N1B4_4369 0.54 +1.1
Or see this region's nucleotide sequence