Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4354 and Pf1N1B4_4355 are separated by 234 nucleotides Pf1N1B4_4355 and Pf1N1B4_4356 are separated by 52 nucleotides
Pf1N1B4_4354 - Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11), at 4,810,521 to 4,811,897
_4354
Pf1N1B4_4355 - Gamma-glutamyl-aminobutyraldehyde dehydrogenase (EC 1.2.1.-), at 4,812,132 to 4,813,622
_4355
Pf1N1B4_4356 - Gamma-glutamyl-putrescine oxidase (EC1.4.3.-), at 4,813,675 to 4,814,973
_4356
Position (kb)
4812
4813
4814 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4811.201 kb on + strand, within Pf1N1B4_4354 at 4811.201 kb on + strand, within Pf1N1B4_4354 at 4811.202 kb on - strand, within Pf1N1B4_4354 at 4811.202 kb on - strand, within Pf1N1B4_4354 at 4811.203 kb on + strand, within Pf1N1B4_4354 at 4811.203 kb on + strand, within Pf1N1B4_4354 at 4811.257 kb on - strand, within Pf1N1B4_4354 at 4811.496 kb on + strand, within Pf1N1B4_4354 at 4811.496 kb on + strand, within Pf1N1B4_4354 at 4811.497 kb on - strand, within Pf1N1B4_4354 at 4811.497 kb on - strand, within Pf1N1B4_4354 at 4811.704 kb on - strand, within Pf1N1B4_4354 at 4811.784 kb on + strand at 4811.784 kb on + strand at 4811.784 kb on + strand at 4811.799 kb on + strand at 4811.799 kb on + strand at 4811.871 kb on + strand at 4811.871 kb on + strand at 4811.871 kb on + strand at 4811.872 kb on - strand at 4811.872 kb on - strand at 4811.880 kb on + strand at 4811.880 kb on + strand at 4812.024 kb on - strand at 4812.103 kb on + strand at 4812.103 kb on + strand at 4812.103 kb on + strand at 4812.103 kb on + strand at 4812.103 kb on + strand at 4812.103 kb on + strand at 4812.103 kb on + strand at 4812.103 kb on + strand at 4812.104 kb on - strand at 4812.104 kb on - strand at 4812.107 kb on + strand at 4812.107 kb on + strand at 4812.107 kb on + strand at 4812.107 kb on + strand at 4812.108 kb on - strand at 4812.181 kb on + strand at 4812.181 kb on + strand at 4812.182 kb on - strand at 4812.361 kb on + strand, within Pf1N1B4_4355 at 4812.544 kb on - strand, within Pf1N1B4_4355 at 4813.194 kb on + strand, within Pf1N1B4_4355 at 4813.194 kb on + strand, within Pf1N1B4_4355 at 4813.194 kb on + strand, within Pf1N1B4_4355 at 4813.426 kb on + strand, within Pf1N1B4_4355 at 4813.500 kb on + strand at 4813.587 kb on + strand at 4813.587 kb on + strand at 4813.587 kb on + strand at 4813.587 kb on + strand at 4813.587 kb on + strand at 4813.587 kb on + strand at 4813.587 kb on + strand at 4813.588 kb on - strand at 4813.588 kb on - strand at 4813.588 kb on - strand at 4813.620 kb on + strand at 4813.629 kb on + strand at 4813.630 kb on - strand at 4813.675 kb on - strand at 4813.675 kb on - strand at 4813.706 kb on + strand at 4813.706 kb on + strand at 4813.706 kb on + strand at 4813.707 kb on - strand at 4813.715 kb on - strand at 4813.717 kb on + strand at 4814.197 kb on + strand, within Pf1N1B4_4356 at 4814.518 kb on + strand, within Pf1N1B4_4356 at 4814.518 kb on + strand, within Pf1N1B4_4356 at 4814.519 kb on - strand, within Pf1N1B4_4356 at 4814.519 kb on - strand, within Pf1N1B4_4356 at 4814.519 kb on - strand, within Pf1N1B4_4356 at 4814.539 kb on + strand, within Pf1N1B4_4356 at 4814.540 kb on - strand, within Pf1N1B4_4356
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 4,811,201 + Pf1N1B4_4354 0.49 +0.8 4,811,201 + Pf1N1B4_4354 0.49 -1.8 4,811,202 - Pf1N1B4_4354 0.49 +1.2 4,811,202 - Pf1N1B4_4354 0.49 +1.3 4,811,203 + Pf1N1B4_4354 0.50 +0.2 4,811,203 + Pf1N1B4_4354 0.50 +0.5 4,811,257 - Pf1N1B4_4354 0.53 -0.6 4,811,496 + Pf1N1B4_4354 0.71 +0.2 4,811,496 + Pf1N1B4_4354 0.71 +0.4 4,811,497 - Pf1N1B4_4354 0.71 -0.5 4,811,497 - Pf1N1B4_4354 0.71 +1.3 4,811,704 - Pf1N1B4_4354 0.86 +0.2 4,811,784 + -1.8 4,811,784 + +2.5 4,811,784 + +0.3 4,811,799 + +0.8 4,811,799 + +1.3 4,811,871 + -1.9 4,811,871 + -0.7 4,811,871 + -2.9 4,811,872 - -0.7 4,811,872 - -0.8 4,811,880 + +0.1 4,811,880 + +0.2 4,812,024 - +0.3 4,812,103 + +0.5 4,812,103 + -0.3 4,812,103 + -2.3 4,812,103 + +0.5 4,812,103 + +1.3 4,812,103 + -0.3 4,812,103 + +0.1 4,812,103 + -2.2 4,812,104 - -0.1 4,812,104 - +0.9 4,812,107 + +0.9 4,812,107 + -1.1 4,812,107 + +0.2 4,812,107 + +0.2 4,812,108 - -1.6 4,812,181 + -0.5 4,812,181 + +0.5 4,812,182 - -0.5 4,812,361 + Pf1N1B4_4355 0.15 +0.8 4,812,544 - Pf1N1B4_4355 0.28 -1.8 4,813,194 + Pf1N1B4_4355 0.71 -2.3 4,813,194 + Pf1N1B4_4355 0.71 -0.5 4,813,194 + Pf1N1B4_4355 0.71 -0.2 4,813,426 + Pf1N1B4_4355 0.87 -0.1 4,813,500 + +0.1 4,813,587 + +1.1 4,813,587 + +0.5 4,813,587 + +0.3 4,813,587 + +1.8 4,813,587 + +1.0 4,813,587 + -0.2 4,813,587 + -0.6 4,813,588 - +0.2 4,813,588 - +2.3 4,813,588 - -1.1 4,813,620 + +0.9 4,813,629 + +0.8 4,813,630 - -1.5 4,813,675 - +0.5 4,813,675 - -0.8 4,813,706 + +0.1 4,813,706 + +0.2 4,813,706 + -2.5 4,813,707 - -1.1 4,813,715 - -1.8 4,813,717 + -0.2 4,814,197 + Pf1N1B4_4356 0.40 -0.6 4,814,518 + Pf1N1B4_4356 0.65 -1.5 4,814,518 + Pf1N1B4_4356 0.65 -0.5 4,814,519 - Pf1N1B4_4356 0.65 -0.5 4,814,519 - Pf1N1B4_4356 0.65 -0.1 4,814,519 - Pf1N1B4_4356 0.65 +0.8 4,814,539 + Pf1N1B4_4356 0.67 -0.6 4,814,540 - Pf1N1B4_4356 0.67 +0.8
Or see this region's nucleotide sequence