Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4352 and Pf1N1B4_4353 are separated by 350 nucleotides Pf1N1B4_4353 and Pf1N1B4_4354 are separated by 280 nucleotides
Pf1N1B4_4352 - Putrescine transport ATP-binding protein PotG (TC 3.A.1.11.2), at 4,807,986 to 4,809,128
_4352
Pf1N1B4_4353 - Gamma-glutamyl-GABA hydrolase (EC 3.5.1.94), at 4,809,479 to 4,810,240
_4353
Pf1N1B4_4354 - Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11), at 4,810,521 to 4,811,897
_4354
Position (kb)
4809
4810
4811 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4809.106 kb on + strand at 4809.106 kb on + strand at 4809.106 kb on + strand at 4809.107 kb on - strand at 4809.107 kb on - strand at 4809.176 kb on + strand at 4809.176 kb on + strand at 4809.176 kb on + strand at 4809.281 kb on - strand at 4809.376 kb on + strand at 4809.377 kb on - strand at 4809.400 kb on + strand at 4809.401 kb on - strand at 4809.403 kb on + strand at 4809.403 kb on + strand at 4809.404 kb on - strand at 4809.404 kb on - strand at 4809.455 kb on + strand at 4809.456 kb on - strand at 4809.488 kb on - strand at 4809.538 kb on + strand at 4809.546 kb on + strand at 4810.159 kb on - strand, within Pf1N1B4_4353 at 4810.413 kb on + strand at 4810.414 kb on - strand at 4810.593 kb on + strand at 4810.594 kb on - strand at 4810.665 kb on - strand, within Pf1N1B4_4354 at 4810.672 kb on - strand at 4810.968 kb on + strand, within Pf1N1B4_4354 at 4811.056 kb on - strand, within Pf1N1B4_4354 at 4811.201 kb on + strand, within Pf1N1B4_4354 at 4811.201 kb on + strand, within Pf1N1B4_4354 at 4811.202 kb on - strand, within Pf1N1B4_4354 at 4811.202 kb on - strand, within Pf1N1B4_4354 at 4811.203 kb on + strand, within Pf1N1B4_4354 at 4811.203 kb on + strand, within Pf1N1B4_4354
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 4,809,106 + +1.5 4,809,106 + -0.7 4,809,106 + -2.3 4,809,107 - +0.2 4,809,107 - -0.5 4,809,176 + -0.6 4,809,176 + -0.7 4,809,176 + -0.1 4,809,281 - +3.5 4,809,376 + -1.5 4,809,377 - -0.5 4,809,400 + -1.5 4,809,401 - -2.5 4,809,403 + +0.3 4,809,403 + +0.5 4,809,404 - -0.7 4,809,404 - +0.5 4,809,455 + -0.5 4,809,456 - +0.5 4,809,488 - +0.1 4,809,538 + -0.5 4,809,546 + +1.1 4,810,159 - Pf1N1B4_4353 0.89 -0.2 4,810,413 + +0.0 4,810,414 - -0.1 4,810,593 + -0.7 4,810,594 - -0.2 4,810,665 - Pf1N1B4_4354 0.10 +0.1 4,810,672 - +1.3 4,810,968 + Pf1N1B4_4354 0.32 -1.3 4,811,056 - Pf1N1B4_4354 0.39 +0.2 4,811,201 + Pf1N1B4_4354 0.49 +0.8 4,811,201 + Pf1N1B4_4354 0.49 -1.8 4,811,202 - Pf1N1B4_4354 0.49 +1.2 4,811,202 - Pf1N1B4_4354 0.49 +1.3 4,811,203 + Pf1N1B4_4354 0.50 +0.2 4,811,203 + Pf1N1B4_4354 0.50 +0.5
Or see this region's nucleotide sequence